Gria3 (NM_001290451) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2900064I19Rik; Glu; GluA3; Glur; Glur-3; GluR-C; GluR-K3; Glur3; Gluralpha3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229110 representing NM_001290451 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCAAAGCGTGCTCCGGGCGGTCTTCTTTTTAGTCCTGGGGCTTTTGGGTCATTCTCACGGAGGAT TCCCCAACACCATCAGCATAGGTGGACTTTTCATGAGAAACACGGTACAGGAGCACAGTGCTTTCCGCTT TGCTGTGCAGTTATACAACACCAACCAGAACACCACTGAGAAGCCCTTCCATTTGAACTACCACGTAGAC CACTTGGATTCCTCCAATAGTTTTTCTGTGACTAATGCTTTCTGCTCCCAGTTCTCCAGAGGGGTGTATG CTATCTTTGGATTCTATGACCAGATGTCAATGAACACACTGACCTCCTTCTGTGGGGCCCTGCATACATC TTTCGTCACACCTAGCTTTCCCACTGATGCCGATGTGCAGTTTGTCATCCAGATGCGCCCAGCCTTAAAG GGTGCCATTCTGAGTCTTCTGGGTTACTACAAGTGGGAGAAGTTTGTGTACCTCTATGACACAGAACGAG GGTTTTCCATCCTGCAAGCAATTATGGAAGCAGCAGTGCAAAACAACTGGCAAGTGACAGCAAGGTCTGT GGGAAACATAAAGGACATCCAGGAATTCAGACGCATCATTGAAGAAATGGACAGAAGGCAGGAAAAACGA TACTTGATTGACTGTGAAGTCGAAAGGATTAACACAATTTTGGAACAGGTTGTGATCCTGGGGAAACATT CAAGAGGTTATCACTACATGCTTGCTAACCTGGGTTTTACTGACATTGTACTGGAAAGAGTCATGCATGG GGGAGCCAACATTACAGGTTTCCAGATTGTCAACAATGAAAACCCAATGGTCCAGCAATTCATACAGCGC TGGGTGAGACTGGATGAAAGGGAATTCCCTGAAGCCAAGAATGCACCACTAAAGTATACATCTGCACTGA CACACGACGCAATACTGGTCATAGCAGAAGCCTTCCGATACCTGAGGAGGCAGCGAGTGGATGTATCCCG CAGAGGCAGTGCTGGAGACTGCTTAGCAAATCCTGCTGTGCCCTGGAGTCAAGGAATTGATATTGAGAGA GCTCTGAAAATGGTGCAAGTACAAGGAATGACTGGAAACATCCAATTTGACACTTATGGACGTAGGACAA ATTATACCATTGATGTATATGAAATGAAAGTCTCAGGCTCTCGAAAAGCTGGTTACTGGAATGAGTATGA AAGGTTTGTGCCCTTCTCAGATCAACAAATCAGCAATGACAGCTCATCCTCCGAGAACAGGACCATCGTA GTGACTACCATTCTGGAATCACCATATGTAATGTATAAAAAGAATCATGAGCAACTGGAAGGAAATGAGC GCTATGAAGGCTATTGTGTCGATTTAGCCTATGAAATAGCCAAACACGTAAGGATCAAATACAAATTGTC CATTGTCGGTGATGGGAAATATGGCGCAAGGGATCCAGAGACTAAAATATGGAATGGCATGGTTGGGGAA CTTGTCTATGGAAGAGCTGATATAGCTGTTGCTCCACTCACTATAACATTGGTCCGTGAAGAAGTCATAG ATTTTTCAAAGCCATTTATGAGCCTGGGGATCTCCATCATGATAAAGAAGCCTCAGAAATCAAAGCCAGG CGTATTTTCATTCCTGGATCCTTTAGCTTATGAAATCTGGATGTGCATTGTCTTCGCTTACATTGGAGTC AGTGTAGTTCTCTTCCTAGTCAGCAGATTTAGCCCTTATGAGTGGCACTTGGAAGACAACAATGAAGAAC CTCGTGACCCACAAAGCCCTCCTGATCCTCCCAATGAATTTGGAATATTTAACAGTCTTTGGTTTTCCTT GGGTGCTTTTATGCAGCAAGGATGTGATATTTCTCCAAGATCACTTTCTGGGCGCATTGTTGGAGGGGTT TGGTGGTTCTTCACCCTGATCATAATCTCTTCCTACACTGCAAACCTTGCTGCTTTCCTGACTGTGGAGA GGATGGTGTCCCCCATAGAGAGCGCTGAAGATTTAGCCAAGCAGACTGAAATTGCATACGGGACCCTGGA CTCTGGTTCAACAAAAGAATTTTTCAGAAGATCCAAAATTGCTGTGTATGAGAAAATGTGGTCTTACATG AAATCCGCAGAGCCATCTGTGTTTACCAAAACAACAGCTGATGGGGTAGCCCGAGTTCGGAAGTCCAAGG GAAAGTTCGCCTTCCTGCTGGAGTCAACCATGAATGAGTACATTGAGCAGAGAAAGCCGTGTGATACGAT GAAAGTTGGTGGAAATCTGGATTCCAAAGGCTATGGTGTGGCAACCCCTAAAGGCTCAGCATTAGGAAAT GCTGTTAACCTGGCAGTATTAAAACTGAATGAGCAAGGCCTCTTGGACAAATTGAAAAACAAATGGTGGT ACGACAAAGGAGAGTGCGGCAGCGGGGGCGGTGACTCCAAGGACAAGACCAGTGCTCTAAGCCTGAGCAA TGTGGCAGGCGTGTTCTATATACTTGTCGGAGGTCTGGGGCTGGCCATGATGGTGGCTTTGATAGAATTC TGTTACAAATCACGGGCAGAGTCCAAACGCATGAAACTCACAAAGAACACCCAAAACTTTAAGCCTGCTC CTGCCACCAACACTCAGAATTACGCTACATACAGAGAAGGCTACAACGTGTATGGAACAGAAAGTGTTAA GATCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290451
Insert Size	2667 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290451.1, NP_001277380.1
RefSeq Size	2705 bp
RefSeq ORF	2667 bp
Locus ID	53623
Gene Summary	This gene encodes a multi-pass transmembrane protein that forms a homotetramer or heterotetramer in neuronal cells. The encoded protein is a ligand-gated ion channel that responds to the neurotransmitter L-glutamate to promote synaptic transmission. Deficiency of this gene leads to behavioral phenotypes. The transcript is subject to RNA editing at codon 769 (AGA->GGA; R->G). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] Transcript Variant: This variant (4) differs in the 5' and 3' UTRs and contains an alternate exon and lacks an exon in the 3' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (b) is the same length but has a different sequence than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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