Gria3 (NM_001290451) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2900064I19Rik; Glu; GluA3; Glur; Glur-3; GluR-C; GluR-K3; Glur3; Gluralpha3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231321 representing NM_001290451 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCAAAGCGTGCTCCGGGCGGTCTTCTTTTTAGTCCTGGGGCTTTTGGGTCATTCTCACGGAGGAT TCCCCAACACCATCAGCATAGGTGGACTTTTCATGAGAAACACGGTACAGGAGCACAGTGCTTTCCGCTT TGCTGTGCAGTTATACAACACCAACCAGAACACCACTGAGAAGCCCTTCCATTTGAACTACCACGTAGAC CACTTGGATTCCTCCAATAGTTTTTCTGTGACTAATGCTTTCTGCTCCCAGTTCTCCAGAGGGGTGTATG CTATCTTTGGATTCTATGACCAGATGTCAATGAACACACTGACCTCCTTCTGTGGGGCCCTGCATACATC TTTCGTCACACCTAGCTTTCCCACTGATGCCGATGTGCAGTTTGTCATCCAGATGCGCCCAGCCTTAAAG GGTGCCATTCTGAGTCTTCTGGGTTACTACAAGTGGGAGAAGTTTGTGTACCTCTATGACACAGAACGAG GGTTTTCCATCCTGCAAGCAATTATGGAAGCAGCAGTGCAAAACAACTGGCAAGTGACAGCAAGGTCTGT GGGAAACATAAAGGACATCCAGGAATTCAGACGCATCATTGAAGAAATGGACAGAAGGCAGGAAAAACGA TACTTGATTGACTGTGAAGTCGAAAGGATTAACACAATTTTGGAACAGGTTGTGATCCTGGGGAAACATT CAAGAGGTTATCACTACATGCTTGCTAACCTGGGTTTTACTGACATTGTACTGGAAAGAGTCATGCATGG GGGAGCCAACATTACAGGTTTCCAGATTGTCAACAATGAAAACCCAATGGTCCAGCAATTCATACAGCGC TGGGTGAGACTGGATGAAAGGGAATTCCCTGAAGCCAAGAATGCACCACTAAAGTATACATCTGCACTGA CACACGACGCAATACTGGTCATAGCAGAAGCCTTCCGATACCTGAGGAGGCAGCGAGTGGATGTATCCCG CAGAGGCAGTGCTGGAGACTGCTTAGCAAATCCTGCTGTGCCCTGGAGTCAAGGAATTGATATTGAGAGA GCTCTGAAAATGGTGCAAGTACAAGGAATGACTGGAAACATCCAATTTGACACTTATGGACGTAGGACAA ATTATACCATTGATGTATATGAAATGAAAGTCTCAGGCTCTCGAAAAGCTGGTTACTGGAATGAGTATGA AAGGTTTGTGCCCTTCTCAGATCAACAAATCAGCAATGACAGCTCATCCTCCGAGAACAGGACCATCGTA GTGACTACCATTCTGGAATCACCATATGTAATGTATAAAAAGAATCATGAGCAACTGGAAGGAAATGAGC GCTATGAAGGCTATTGTGTCGATTTAGCCTATGAAATAGCCAAACACGTAAGGATCAAATACAAATTGTC CATTGTCGGTGATGGGAAATATGGCGCAAGGGATCCAGAGACTAAAATATGGAATGGCATGGTTGGGGAA CTTGTCTATGGAAGAGCTGATATAGCTGTTGCTCCACTCACTATAACATTGGTCCGTGAAGAAGTCATAG ATTTTTCAAAGCCATTTATGAGCCTGGGGATCTCCATCATGATAAAGAAGCCTCAGAAATCAAAGCCAGG CGTATTTTCATTCCTGGATCCTTTAGCTTATGAAATCTGGATGTGCATTGTCTTCGCTTACATTGGAGTC AGTGTAGTTCTCTTCCTAGTCAGCAGATTTAGCCCTTATGAGTGGCACTTGGAAGACAACAATGAAGAAC CTCGTGACCCACAAAGCCCTCCTGATCCTCCCAATGAATTTGGAATATTTAACAGTCTTTGGTTTTCCTT GGGTGCTTTTATGCAGCAAGGATGTGATATTTCTCCAAGATCACTTTCTGGGCGCATTGTTGGAGGGGTT TGGTGGTTCTTCACCCTGATCATAATCTCTTCCTACACTGCAAACCTTGCTGCTTTCCTGACTGTGGAGA GGATGGTGTCCCCCATAGAGAGCGCTGAAGATTTAGCCAAGCAGACTGAAATTGCATACGGGACCCTGGA CTCTGGTTCAACAAAAGAATTTTTCAGAAGATCCAAAATTGCTGTGTATGAGAAAATGTGGTCTTACATG AAATCCGCAGAGCCATCTGTGTTTACCAAAACAACAGCTGATGGGGTAGCCCGAGTTCGGAAGTCCAAGG GAAAGTTCGCCTTCCTGCTGGAGTCAACCATGAATGAGTACATTGAGCAGAGAAAGCCGTGTGATACGAT GAAAGTTGGTGGAAATCTGGATTCCAAAGGCTATGGTGTGGCAACCCCTAAAGGCTCAGCATTAGGAAAT GCTGTTAACCTGGCAGTATTAAAACTGAATGAGCAAGGCCTCTTGGACAAATTGAAAAACAAATGGTGGT ACGACAAAGGAGAGTGCGGCAGCGGGGGCGGTGACTCCAAGGACAAGACCAGTGCTCTAAGCCTGAGCAA TGTGGCAGGCGTGTTCTATATACTTGTCGGAGGTCTGGGGCTGGCCATGATGGTGGCTTTGATAGAATTC TGTTACAAATCACGGGCAGAGTCCAAACGCATGAAACTCACAAAGAACACCCAAAACTTTAAGCCTGCTC CTGCCACCAACACTCAGAATTACGCTACATACAGAGAAGGCTACAACGTGTATGGAACAGAAAGTGTTAA GATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231321 representing NM_001290451 Red=Cloning site Green=Tags(s) MGQSVLRAVFFLVLGLLGHSHGGFPNTISIGGLFMRNTVQEHSAFRFAVQLYNTNQNTTEKPFHLNYHVD HLDSSNSFSVTNAFCSQFSRGVYAIFGFYDQMSMNTLTSFCGALHTSFVTPSFPTDADVQFVIQMRPALK GAILSLLGYYKWEKFVYLYDTERGFSILQAIMEAAVQNNWQVTARSVGNIKDIQEFRRIIEEMDRRQEKR YLIDCEVERINTILEQVVILGKHSRGYHYMLANLGFTDIVLERVMHGGANITGFQIVNNENPMVQQFIQR WVRLDEREFPEAKNAPLKYTSALTHDAILVIAEAFRYLRRQRVDVSRRGSAGDCLANPAVPWSQGIDIER ALKMVQVQGMTGNIQFDTYGRRTNYTIDVYEMKVSGSRKAGYWNEYERFVPFSDQQISNDSSSSENRTIV VTTILESPYVMYKKNHEQLEGNERYEGYCVDLAYEIAKHVRIKYKLSIVGDGKYGARDPETKIWNGMVGE LVYGRADIAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGV SVVLFLVSRFSPYEWHLEDNNEEPRDPQSPPDPPNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGV WWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLDSGSTKEFFRRSKIAVYEKMWSYM KSAEPSVFTKTTADGVARVRKSKGKFAFLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGVATPKGSALGN AVNLAVLKLNEQGLLDKLKNKWWYDKGECGSGGGDSKDKTSALSLSNVAGVFYILVGGLGLAMMVALIEF CYKSRAESKRMKLTKNTQNFKPAPATNTQNYATYREGYNVYGTESVKI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290451
ORF Size	2664 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290451.2
RefSeq Size	2705 bp
RefSeq ORF	2667 bp
Locus ID	53623
MW	100.8 kDa
Gene Summary	This gene encodes a multi-pass transmembrane protein that forms a homotetramer or heterotetramer in neuronal cells. The encoded protein is a ligand-gated ion channel that responds to the neurotransmitter L-glutamate to promote synaptic transmission. Deficiency of this gene leads to behavioral phenotypes. The transcript is subject to RNA editing at codon 769 (AGA->GGA; R->G). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.