Zhx1 (NM_009572) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	3110006I21Rik; mKIAA4149
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229091 representing NM_009572 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAGCAGACGAAAATCAACAACACCTTGCATGGTCCTTGCCAGTGAGCAGGATCCAGATCTTGAGC TGATATCGGATTTGGATGAAGGCCCTCCCATCCTTACACCTGTAGAGAATGCTAAAGCAGAGAGTGTCTC CAGCGATGAAGAAGTTCACGGATCTGTGGATTCTGACAATCAGCAAAATAAGAAAGTGGAAGGGGGCTAT GAATGTAAATATTGTACTTTTCAAACTCCAGATCTAAATATGTTTACTTTCCATGTAGATTCAGAACATC CCAATGTTGTGCTGAATTCCTCCTATGTTTGTGTTGAATGCAATTTTCTTACCAAAAGGTATGATGCACT TTCTGAGCATAATCTGAAATACCACCCAGGAGAAGAAAATTTCAAGCTGACTATGGTGAAGCGGAATAAC CAGACAATCTTTGAACAGACAATAAATGATCTAACTTTTGATGGCAGTTTTGTTAAAGAGGAGAATACAG AACAAGGAGAATCCATAGATGTTTCTTCTTCGGGAATCTCCATTAGTAAAACTCCCATCATGAAAATGAT GAAAAATAAGGTAGAGAACAAACGGATTACAGTCCACCATAACTCAGCTGAGGGCACTTCTGAAGAGAAA GAGAATGGAGTAAAAGCAAGCCAGGAAGAAAATGCAGAAAGTGTGAGTTCTTCAGCTTTAGAATCCAATA CAAGTACTTCCACCATCAACAGAGTGCATCCCAGTCCTGCCAGCACAGTGGTGACTCCCACAGCTGTTCT TCCTGGGTTAGCACAGGTGATAACTGCGGTGTCTGCTCAACAGAACTCAAACTTGCTCCCTAAAGTCTTA ATCCCCGTTAATAGCATTCCTACCTACAATGCTGCATTGGATAACAATCCTCTTCTACTTAATACCTACA ACAAATTCCCTTATCCTACAATGTCAGAAATTACAGTTCTTTCTGCTCAAGCAAAATATACAGAGGAACA AATCAAGATCTGGTTTTCAGCCCAACGTTTAAAGCATGGTGTTAGTTGGACTCCTGAAGAAGTAGAGGAG GCGAGAAGAAAACAATTCAATGGAACAGTACATACTGTACCTCAGACCATAACTGTTATTCCTACACACA TTTCCACAGGGAGTAACGGGTTACCATCTATCTTACAGACATGCCAAATTGTTGGTCAGCCAGGTCTGGT CCTTACTCAAGTAGCTGGAACAAACACCTTGCCAGTAACAGCACCCATTGCCCTGACAGTGGCAGGGGTT CCAAATCAAACAAATGTACAAAAGAGCCAAGTGCCTGCTGCTCAGCCTGCTACAGACACAAAACCAGCCA CAGCAGCAGTTCCATCCTCGCCAAGTGTCAGACCTGAAGCTGCACTAGTGAACCCAGATTCATTTGGTAT TCGGGCCAAAAAGACTAAAGAGCAGCTGGCAGAACTGAAAGTTAGCTACCTAAAAAATCAGTTTCCCCAT GACTCAGAGATCATCAGGCTTATGAAAATAACAGGCCTTACCAAAGGCGAGATTAAAAAATGGTTTAGTG ATACAAGGTACAACCAGAGAAATTCAAAGAGTAACCAGTGCTTACATCTCAACAATGACTCCTCTGCCAC TATCATCATAGACTCCAGTGATGAAACCCCAGAACCCCCAGCGGCAGCTGCTTCACAGCAGAAACAGTCC TGGAATCCCTTTCCTGACTTTGCTCCCCAGAAGTTTAAAGAGAAAACTGCAGAGCAACTCCGTGCCCTTC AGGCAAGTTTTCTCAACAGTTCTGTACTTACTGATGAAGAAGTAAATAGGTTAAGAGCGCAAACCAAACT TACTAGAAGAGAAATTGATGCTTGGTTTACAGAGAAAAATAAAACAAAAGCTTTAAAGGATGAGAAAATA GAAGTAGATGAAAGTAATGTAGGTAGTTCCAAAGAAGAGCCTGGAGAAAGTTCTCCTGGAGATGAAACAG TTGCACCTAAGTCAGGAGGGACAGGCAAGATATGTAAGAAAACACCAGAGCAGCTGCACATGCTTAAAAG TGCATTTGTCCGAACACAGTGGCCATCAGCAGAAGAGTATGACAAGCTGGCTGAAGAGAGTGGCCTTGCC AGAACAGATATAGTTAGTTGGTTTGGGGATACCCGTTATGCTTGGAAGAATGGAAACTTGAAATGGTATT ACTACTATCAAAGCTCCAATTCAAGTAGTTTGAATGGTCTATCTTCCCTTAGGAGAAGAGGGAGAGGCAG ACCCAAAGGAAGGGGCAGGGGCAGACCACGTGGTCGGCCCAGAGGTGGAAAAAGAATGAACACCTGGGAC AGGGTACCATCGCTTATAAAGTTTAAAACTGGAACAGCAATACTTAAGGATTATTACCTGAAGCACAAAT TTCTTAATGAGCAAGACCTTGATGAACTTGTCAACAGATCACACATGGGTTATGAGCAGGTCAGAGAGTG GTTTGCAGAAAGACAGAGAAGATCTGAGTTAGGTATAGAATTATTTGAGGAAAATGAGGAGGAAGATGAA GTTGTTGATGATCAGGAAGAAGATGAAGAAGAAACAGATGATAGTGACACTTGGGAACCCCCACGACATG TGAAGCGGAAGCTTTCTAAATCGGATGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_009572
Insert Size	2622 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_009572.4, NP_033598.2
RefSeq Size	5105 bp
RefSeq ORF	2622 bp
Locus ID	22770
UniProt ID	P70121
Gene Summary	Acts as a transcriptional repressor. Increases DNMT3B-mediated repressive transcriptional activity when DNMT3B is tethered to DNA. May link molecule between DNMT3B and other co-repressor proteins (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) differs in the 5' UTR, compared to variant 2. Both variants 1 and 2 encode the same protein.

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