Nlrp6 (NM_133946) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI504961; Avr; N; Nalp6; Navr; Navr/Avr; Non-AVR; Pypaf5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229080 representing NM_133946 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGCCGCTGGAGCCTCCTGCTCCAGCGTGGATGCAGTGGCAAGGGAGCTGCTTATGGCTACACTGG AGGAGCTGAGCCAGGAGCAGCTGAAGCGCTTCCGCCACAAGCTAAGGGATGCGCCCCTGGATGGCCGCAG CATCCCGTGGGGGCGACTGGAGCGCTCAGACGCTGTGGACCTTGTCGACAAGCTCATTGAGTTCTATGAG CCAGTGCCAGCTGTAGAAATGACCCGGCAGGTCCTGAAGAGGTCGGATATTCGAGATGTGGCCTCGCGAC TTAAGCAACAGCAGCTACAGAAGCTCGGCCCCACCTCGGTGCTTCTCTCCGTGTCAGCGTTCAAGAAGAA GTACCGCGAGCACGTGCTACGACAGCATGCTAAGGTGAAGGAGAGGAATGCTCGCTCTGTGAAGATCAAC AAGCGCTTCACCAAGCTGCTCATCGCTCCGGGGACCGGCGCTGTGGAGGACGAGCTGCTGGGGCCGTTGG GGGAGCCGGAGCCCGAACGCGCTCGGCGCTCGGACACGCACACTTTTAATCGGCTCTTCCGGGGAAATGA TGAAGAGAGCTCGCAGCCGCTGACCGTGGTGCTGCAGGGCCCGGCAGGCATCGGCAAAACCATGGCCGCC AAGAAAATCCTCTATGACTGGGCAGCAGGTAAGCTATACCACAGCCAGGTGGACTTTGCCTTCTTCATGC CGTGCGGCGAGCTGCTGGAGCGGCCGGGAAAGCGCAGCTTGGCGGACCTGGTGCTGGACCAGTGCCCGGA CCGCGCTTGGCCAGTGAAGCGGATACTGGCGCAACCCAATCGCCTACTGTTCATCCTGGACGGTGCAGAC GAGCTGCCTACTTTACCATCCTCAGAGGCCACTCCCTGCAAAGACCCTTTGGAGGCTACAAGTGGCTTGA GAGTGCTGAGCGGGCTGCTGAGCCAGGAGCTGTTACCAGGAGCTCGCTTGCTAGTGACTACACGCCATGC CGCTACAGGGAGGCTGCAGGGCAGATTGTGCTCGCCACAGTGCGCAGAAATACGCGGCTTCTCAGACAAA GACAAAAAAAAGTATTTCTTCAAGTTCTTCCGGGACGAGAGGAAGGCAGAGCGCGCTTACCGCTTCGTGA AAGAGAACGAGACGCTGTTTGCACTGTGCTTTGTGCCCTTCGTGTGCTGGATCGTGTGCACAGTGCTGCA GCAGCAGCTAGAGCTGGGCCGGGATCTCTCTCGTACCTCCAAGACCACTACTTCTGTGTACCTGCTCTTC ATCACCAGCATGCTGAAGTCTGCAGGCACCAATGGACCCCGGGTTCAGGGAGAGCTGAGAACGCTGTGTC GCCTGGCCAGGGAGGGCATCCTGGATCATCATAAAGCACAATTCTCAGAAGAGGACCTGGAGAAATTGAA GCTTCGGGGTTCCCAAGTTCAGACAATATTTCTCAACAAGAAAGAGATACCAGGAGTGCTAAAAACTGAG GTCACCTACCAGTTCATTGACCAGAGCTTCCAGGAGTTCTTGGCTGCATTGTCATACCTACTAGAAGCTG AGCGAACTCCGGGGACGCCCGCAGGCGGTGTGCAGAAGCTCCTGAACTCTGACGCGGAGCTACGTGGTCA TCTTGCACTGACCACCCGATTCCTCTTTGGCCTGCTAAATACAGAGGGGCTTCGTGACATTGGAAACCAT TTTGGCTGTGTGGTGCCAGATCATGTGAAAAAGGACACCCTGCGGTGGGTACAAGGACAGAGCCACCCCA AGGGGCCACCAGTAGGGGCAAAAAAGACTGCTGAGCTGGAGGACATCGAGGACGCAGAGGAGGAGGAGGA GGAAGAGGAGGACCTCAATTTTGGACTGGAGCTGCTGTACTGCCTGTATGAGACACAGGAGGAGGATTTT GTTCGACAGGCTCTCAGCAGCCTTCCAGAGATAGTACTGGAGCGAGTCAGGTTGACCCGCATGGACCTTG AAGTTCTGAATTACTGCGTGCAGTGCTGCCCAGATGGCCAGGCTCTGAGACTGGTGAGCTGTGGGCTGGT GGCGGCAAAGGAGAAGAAGAAGAAGAAGAAGAGCCTGGTGAAGCGGCTGAAGGGCTCTCAAAGCACCAAG AAACAACCCCCAGTCTCCTTGCTGCGTCCACTCTGTGAGACAATGACTACCCCGAAATGTCATCTGAGTG TTCTGATCTTGTCACACTGCAGACTCCCTGATGCAGTTTGCCGAGACCTTTCCGAGGCCCTGAAGGTAGC TCCTGCCCTAAGGGAGTTGGGCCTCCTCCAGAGCCGGCTCACCAACACAGGCCTGCGTTTACTGTGCGAA GGCCTGGCTTGGCCCAAGTGCCAGGTGAAGACACTCAGGATGCAGCTGCCTGACCTCCAAGAGGTGATCA ATTACTTGGTCATCGTGCTCCAGCAGAGCCCAGTCCTGACCACTCTGGACCTCAGTGGCTGTCAGCTGCC TGGCGTCATTGTGGAACCTCTGTGTGCAGCCCTGAAGCACCCTAAATGCTCCCTAAAGACCCTCAGTCTG ACCTCTGTGGAGCTGAGTGAGAACTCACTGAGGGATCTTCAAGCTGTGAAGACATCAAAGCCAGATCTGT CCATCATATATTCAAAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_133946
Insert Size	2610 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_133946.2, NP_598707.2
RefSeq Size	4438 bp
RefSeq ORF	2610 bp
Locus ID	101613
UniProt ID	Q91WS2
Gene Summary	The protein encoded by this gene binds arginine-vasopressin and may be involved in the arginine-vasopressin-mediated regulation of renal salt-water balance. The encoded protein also mediates inflammatory responses in the colon to allow recovery from intestinal epithelial damage and protects against tumorigenesis and the development of colitis. Finally, this protein can increase activation of NF-kappa-B, activation of CASP1 through interaction with ASC, and cAMP accumulation. [provided by RefSeq, Feb 2013]

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