Dnm1 (NM_001301737) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI838169; Dnm; Ftf; Ftfl; mKIAA4093
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229045 representing NM_001301737 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAACCGCGGCATGGAAGACCTCATCCCGCTGGTTAACCGGTTACAGGACGCCTTCTCCGCCATCG GCCAGAACGCGGACCTCGACCTGCCGCAGATCGCCGTGGTAGGCGGCCAGAGCGCCGGCAAGAGCTCGGT GCTGGAGAATTTCGTGGGCAGGGACTTCTTGCCCCGAGGATCTGGCATCGTCACCCGGCGTCCCCTGGTC CTGCAGCTGGTTAATTCTACCACAGAATATGCCGAGTTCCTGCACTGCAAGGGGAAGAAATTCACCGACT TCGAGGAGGTGCGCCTGGAGATCGAGGCTGAGACCGATCGAGTCACCGGCACCAACAAGGGCATTTCGCC AGTGCCCATCAACCTGCGGGTCTACTCGCCCCATGTGCTGAACCTGACTCTAGTGGACCTGCCAGGAATG ACCAAGGTCCCAGTTGGGGACCAACCTCCTGATATCGAGTTCCAGATCCGGGACATGCTTATGCAGTTCG TCACTAAGGAGAACTGCCTTATCCTGGCTGTGTCCCCTGCCAACTCGGATTTGGCCAACTCTGATGCCCT CAAGATCGCTAAGGAGGTGGACCCCCAGGGTCAGCGCACCATTGGGGTCATCACCAAGTTGGACCTGATG GACGAGGGCACAGATGCGCGGGACGTGCTAGAGAACAAGCTGCTCCCTTTGCGCAGAGGTTACATCGGCG TGGTGAACCGGAGCCAGAAGGACATAGACGGCAAGAAGGACATCACAGCCGCCTTGGCTGCAGAGCGCAA ATTCTTCCTCTCTCACCCATCCTACCGCCACTTGGCTGACCGCATGGGCACACCCTACCTGCAGAAGGTC CTCAATCAGCAATTGACCAACCACATCCGGGACACACTGCCGGGACTTCGGAACAAGCTGCAGAGCCAGC TGCTGTCCATTGAGAAGGAGGTGGACGAGTACAAGAACTTCCGACCGGATGACCCAGCGCGCAAGACCAA GGCCCTGCTGCAGATGGTCCAGCAGTTTGCAGTGGACTTCGAGAAGCGCATCGAGGGTTCTGGAGACCAG ATTGACACTTACGAGCTGTCAGGTGGAGCCCGCATTAACCGGATCTTCCATGAACGCTTCCCCTTTGAGC TGGTTAAGATGGAGTTTGATGAGAAGGAACTGCGAAGGGAGATCAGCTATGCTATCAAAAATATCCATGG CATCAGGACGGGCCTCTTCACACCTGACCTCGCTTTTGAAGCCACAGTGAAAAAGCAGGTGCAGAAGCTC AAAGAGCCCAGTATCAAGTGTGTGGACATGGTAGTCAGTGAACTCACGTCCACCATCAGAAAGTGTAGTG AAAAGCTGCAGCAATACCCGCGTCTGCGGGAGGAGATGGAGCGAATTGTGACCACCCACATCCGGGAACG TGAGGGCCGCACCAAGGAGCAGGTCATGCTTCTCATCGACATTGAGCTGGCTTACATGAATACCAACCAC GAAGACTTCATAGGCTTTGCCAATGCTCAGCAGAGAAGCAACCAGATGAACAAGAAGAAGACTTCAGGGA ACCAGGATGAGATTCTGGTCATTCGAAAGGGGTGGTTGACCATCAACAACATCGGCATCATGAAGGGAGG CTCCAAGGAGTACTGGTTTGTGCTGACTGCTGAGAATCTGTCCTGGTACAAGGATGATGAGGAGAAAGAG AAGAAGTACATGCTGTCTGTGGACAATCTGAAGCTGCGTGATGTGGAGAAGGGCTTCATGTCAAGCAAGC ATATTTTTGCCCTCTTCAACACAGAGCAGAGGAATGTCTACAAGGATTACCGGCAGCTGGAACTGGCCTG TGAGACACAGGAGGAGGTGGACAGTTGGAAGGCTTCCTTCCTGAGGGCTGGCGTGTACCCTGAGCGTGTT GGGGACAAAGAGAAAGCCAGTGAGACCGAGGAGAACGGCTCTGACAGCTTCATGCACTCGATGGACCCTC AGCTGGAGCGCCAGGTGGAGACCATCCGGAACCTGGTAGACTCGTACATGGCCATTGTCAACAAGACTGT GCGGGACCTCATGCCCAAGACCATCATGCACCTCATGATCAACAACACCAAGGAGTTTATCTTCTCTGAG CTGCTGGCCAACCTGTACTCTTGCGGGGACCAGAACACACTGATGGAAGAATCGGCCGAGCAGGCTCAGC GGCGCGACGAGATGCTGCGCATGTACCACGCACTGAAGGAGGCGCTCAGTATTATCGGCGACATCAACAC GACCACCGTCAGCACGCCCATGCCCCCGCCCGTGGACGACTCCTGGCTGCAGGTGCAGAGCGTACCGGCC GGACGCAGATCGCCCACGTCCAGCCCCACGCCGCAGCGCCGAGCCCCCGCCGTGCCCCCAGCCCGGCCCG GATCGCGGGGCCCTGCTCCTGGGCCTCCGCCTGCTGGATCCGCCCTGGGGGGGGCGCCCCCCGTGCCCTC CAGGCCGGGGGCTTCCCCTGACCCCTTTGGCCCCCCTCCCCAGGTGCCCTCGCGCCCCAACCGCGCCCCG CCTGGGGTCCCCAGAATCACTATCAGTGACCCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001301737
Insert Size	2556 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301737.1, NP_001288666.1
RefSeq Size	3295 bp
RefSeq ORF	2556 bp
Locus ID	13429
UniProt ID	P39053
Gene Summary	This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein is a GTPase which is required for membrane recycling, including vesicle endocytosis in neurons. It may also be involved in cellular fission via association with microtubules and actin filaments. Mutations in this gene have been shown to cause seizures. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014] Transcript Variant: This variant (2) has multiple differences in the coding region, one of which results in a frameshift, compared to variant 1. The resulting protein (isoform 2) has a distinct C-terminus and is shorter than isoform 1. This variant contains the alternate 'a' exon in the central coding region and the 'b' splicing pattern in the 3' coding region (PMID:10398046, PMID:20700442).

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