Pde10a (NM_001290707) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229044 representing NM_001290707 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGAAGAAGCGCAAAGCCCTCGAGGGTGGAGGAGAACCCCAGCTCCCAGAGGAGGAACCCACTGCCT GGTTTGGGGGCAGCAGCGAGGAGCCAGGCTGCCTCCCATTCGCACTTAAAGGTGGGAGCAAGGGCCCCGC TCTATTGGCACTTCGAAACCGCACAGACTCTCGGGGGCAGATGAGCAATGACTCCACAGAAGGCACCGTG GGCTCCTGCAACGCAACAGGTTTGACGGATGAAAAGGTGAAGGCCTATCTTTCTCTCCATCCCCAGGTAT TAGATGAATTTGTTTCTGAAAGTGTTAGTGCAGAGACTGTGGAAAAGTGGCTGAAGAGGAAAACCAACAA AGCAAAAGATGAACCATCTCCCAAGGAAGTCAGCAGGTACCAGGATACGAATATGCAGGGAGTCGTGTAC GAGCTGAACAGCTACATAGAGCAGCGCCTGGACACGGGCGGGGACAACCACCTGCTCCTCTATGAGCTCA GCAGCATCATCAGGATAGCCACAAAAGCCGACGGATTTGCACTGTACTTCCTTGGAGAGTGCAATAATAG CCTGTGTGTGTTCATACCACCCGGGATGAAGGAAGGCCAACCCCGGCTCATCCCTGCAGGGCCCATCACC CAGGGTACCACCATCTCTGCCTACGTGGCCAAGTCTAGGAAGACGTTGTTGGTAGAGGATATCCTTGGGG ATGAGCGATTTCCTCGAGGTACTGGCCTGGAATCAGGAACCCGCATCCAGTCTGTTCTTTGCTTGCCCAT TGTCACTGCCATTGGAGACTTGATTGGCATCCTTGAACTGTACAGGCACTGGGGCAAAGAGGCCTTCTGC CTCAGCCATCAGGAGGTTGCAACAGCCAATCTTGCTTGGGCTTCCGTAGCAATACACCAGGTGCAGGTGT GTAGAGGTCTCGCCAAACAGACCGAACTGAATGACTTCCTACTCGACGTATCAAAGACATACTTTGATAA CATAGTTGCCATAGACTCTCTACTTGAACACATCATGATATATGCAAAAAATCTAGTGAACGCCGACCGC TGCGCGCTCTTCCAGGTGGACCACAAGAACAAGGAGCTGTACTCGGACCTGTTTGACATTGGGGAGGAGA AGGAGGGGAAGCCCATCTTCAAGAAGACCAAGGAGATCAGATTTTCCATTGAGAAAGGGATTGCTGGTCA AGTGGCAAGAACAGGCGAAGTCTTGAACATTCCCGATGCCTACGCGGACCCTCGCTTTAACAGGGAGGTG GACCTGTACACAGGCTACACCACGAGGAACATTCTGTGTATGCCCATAGTGAGCCGAGGCAGCGTGATTG GCGTGGTGCAGATGGTGAACAAGATCAGCGGTAGCGCCTTCTCCAAGACAGACGAGAACAACTTCAAGAT GTTTGCTGTCTTCTGCGCACTGGCCTTGCACTGTGCTAACATGTACCACAGGATCCGCCACTCAGAATGC ATCTACAGGGTTACCATGGAGAAGCTTTCCTACCACAGCATCTGCACCTCCGAGGAGTGGCAAGGCCTCA TGCGCTTCAACCTACCAGCACGCATCTGCCGGGACATCGAGCTATTCCACTTTGACATTGGTCCTTTCGA GAACATGTGGCCTGGGATCTTTGTCTACATGATCCATCGGTCTTGTGGGACATCCTGTTTTGAACTTGAA AAATTGTGCCGTTTTATCATGTCTGTGAAGAAGAACTATCGGCGGGTTCCTTACCACAACTGGAAGCATG CAGTCACGGTGGCACACTGCATGTATGCCATACTTCAAAACAACAATGGCCTCTTCACAGACCTCGAGCG CAAAGGCCTGCTAATTGCGTGTCTGTGCCATGACCTGGACCACAGGGGCTTCAGTAACAGCTACCTGCAG AAGTTCGACCACCCCCTGGCGGCGCTGTACTCCACCTCCACCATGGAGCAACACCACTTCTCCCAGACGG TGTCCATCCTTCAGCTGGAAGGGCACAATATCTTCTCCACCCTGAGCTCCAGCGAGTACGAGCAGGTGCT GGAGATCATCCGCAAAGCCATCATCGCCACCGACCTCGCCCTATACTTTGGGAACAGGAAGCAGTTGGAG GAGATGTACCAGACAGGGTCGCTGAACCTCCACAACCAGTCCCATCGAGACCGTGTCATCGGCTTGATGA TGACTGCCTGTGATCTTTGCTCTGTGACCAAACTATGGCCAGTTACAAAATTGACAGCGAATGATATATA TGCAGAATTCTGGGCTGAGGGTGATGAGATGAAGAAGCTGGGCATACAGCCCATTCCTATGATGGACAGA GACAAGCGAGATGAAGTCCCTCAAGGGCAGCTCGGATTCTACAATGCTGTGGCCATTCCCTGCTATACCA CCTTGACGCAGATCCTCCCACCCACAGAGCCTCTGCTGAAGGCCTGCAGGGATAACCTCAATCAGTGGGA GAAGGTAATTCGCGGGGAAGAGACAGCAATGTGGATTTCAGGCCCAGGCCCGGCGCCTAGCAAGAGCACA CCTGAGAAGCTGAACGTGAAGGTTGAAGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290707
Insert Size	2553 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290707.1, NP_001277636.1
RefSeq Size	7763 bp
RefSeq ORF	2553 bp
Locus ID	23984
UniProt ID	Q8CA95
Gene Summary	Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate. May play a critical role in regulating cAMP and cGMP levels in the striatum, a region of the brain that contributes to the control of movement and cognition.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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