Ldlr (NM_001252658) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Hlb301
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228983 representing NM_001252658 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCACCGCGGATCTGATGCGTCGCTGGGTCATCGCCCTGCTCCTGGCTGCTGCCGGAGTTGCAGCAG AAGACTCATGCAGCAGGAACGAGTTCCAGTGTAGAGACGGAAAATGCATCGCTAGCAAGTGGGTGTGCGA TGGCAGCCCCGAGTGCCCGGATGGCTCCGATGAGTCCCCAGAGACATGCATGTCTGTCACCTGTCAGTCC AATCAATTCAGCTGTGGAGGCCGTGTCAGCCGATGCATTCCTGACTCCTGGAGATGTGATGGACAGGTAG ACTGTGAAAATGACTCAGACGAACAAGGCTGTCCCCCCAAGACGTGCTCCCAGGATGACTTCCGATGCCA GGATGGCAAGTGCATCTCCCCGCAGTTTGTGTGTGATGGAGACCGAGATTGCCTAGATGGCTCTGATGAG GCCCACTGCCAGGCCACCACTTGTGGCCCCGCCCACTTCCGCTGCAACTCATCCATATGCATCCCCAGTC TTTGGGCCTGCGACGGGGATGTCGACTGTGTTGACGGCTCCGATGAGTGGCCACAGAACTGCCAGGGCCG AGACACGGCCTCCAAAGGCGTTAGCAGCCCCTGCTCCTCCCTGGAGTTCCACTGTGGTAGCAGTGAGTGT ATCCATCGCAGCTGGGTCTGTGACGGCGAGGCAGACTGCAAGGACAAGTCAGATGAGGAGCACTGCGCGG TGGCCACCTGCCGACCTGATGAATTCCAGTGTGCAGATGGCTCCTGCATTCACGGTAGCCGCCAGTGTGA CCGTGAACATGACTGCAAGGACATGAGCGACGAGCTCGGCTGCGTCAATGTGACACAGTGTGATGGCCCC AACAAGTTCAAGTGTCACAGTGGGGAGTGCATCAGCTTGGACAAGGTGTGCGACTCCGCCCGCGACTGCC AGGACTGGTCGGATGAGCCCATCAAGGAGTGCAAGACCAACGAGTGTTTGGACAACAATGGTGGCTGTTC CCACATCTGCAAGGACCTCAAGATTGGCTCTGAGTGCCTGTGTCCCAGCGGCTTCCGGTTGGTGGACCTC CACAGGTGTGAAGATATTGACGAGTGTCAGGAGCCAGACACCTGCAGCCAGCTCTGTGTGAACCTGGAAG GCAGCTACAAGTGTGAGTGCCAGGCCGGCTTCCACATGGACCCACACACCAGGGTCTGCAAGGCTGTGGG CTCCATAGGCTATCTGCTCTTCACCAACCGCCACGAGGTCCGGAAGATGACCCTGGACCGCAGCGAGTAC ACCAGTCTGCTCCCCAACCTGAAGAATGTGGTGGCTCTCGACACGGAGGTGACCAACAATAGAATCTACT GGTCCGACCTGTCCCAAAAAAAGATCTACAGCGCCCTGATGGACCAGGCCCCTAACTTGTCCTACGACAC CATCATCAGTGAGGACCTGCATGCCCCTGACGGGCTGGCGGTAGACTGGATCCACCGCAACATCTACTGG ACAGATTCAGTCCCAGGCAGCGTATCTGTGGCTGACACCAAGGGCGTAAAGAGGAGGACACTGTTCCAAG AGGCAGGGTCCAGACCCAGAGCCATCGTAGTGGACCCTGTGCATGGCTTCATGTACTGGACAGATTGGGG AACACCCGCCAAGATCAAGAAAGGGGGTTTGAATGGTGTGGACATCCACTCACTGGTGACCGAAAACATC CAGTGGCCAAATGGCATCACACTAGATCTTTCCAGTGGCCGTCTCTATTGGGTTGATTCCAAACTCCACT CTATCTCCAGCATCGATGTCAATGGGGGCAATCGGAAAACCATTTTGGAGGATGAGAACCGGCTGGCCCA CCCCTTCTCCTTGGCCATCTATGAGGACAAAGTGTATTGGACAGATGTCATAAACGAAGCCATTTTCAGT GCCAATCGACTCACGGGTTCAGATGTGAATTTGGTGGCTGAAAACCTCTTGTCCCCGGAGGACATTGTCC TGTTCCACAAGGTCACACAGCCTAGAGAAGTCGACACTGTACTGACCACCCAGGGGACATCCGCCGTCCG GCCTGTGGTCACCGCATCAGCTACCAGGCCACCGAAGCACAGTGAGGATCTCTCAGCTCCCAGTACTCCT AGGCAGCCTGTGGACACCCCAGGGCTCAGCACAGTGGCGTCAGTGACAGTGTCCCACCAAGTCCAGGGTG ACATGGCTGGCAGAGGGAATGAGGAGCAGCCACATGGTATGAGGTTCCTGTCCATCTTCTTCCCTATTGC ACTGGTTGCCCTCCTTGTCCTTGGGGCCGTCCTGCTGTGGAGGAACTGGCGGCTGAAGAACATCAACAGC ATAAACTTTGACAACCCAGTCTACCAGAAGACCACAGAGGACGAGCTCCACATTTGCCGAAGCCAGGATG GCTATACCTACCCCTCAAGACAGATGGTCAGCCTGGAGGACGATGTGGCATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252658
Insert Size	2433 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252658.1, NP_001239587.1
RefSeq Size	4393 bp
RefSeq ORF	2433 bp
Locus ID	16835
UniProt ID	P35951
Gene Summary	Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.