Unk (NM_001286006) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	B230379M23Rik; mKIAA1753; Zc3h5; Zc3hdc5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228952 representing NM_001286006 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGAAGGGCCCCGGGCCCGGCGGCTCGGCAGCTTCCTCGGCGCCCCCGGCCGCTACCGCTCAGGTGC TGCAGGCACAGCCCGAGAAACCGCAGCACTACACGTACCTGAAGGAATTCCGCACAGAGCAGTGCCCACT CTTCGTGCAGCACAAATGCACACAGCATCGGCCCTATACCTGCTTCCACTGGCACTTTGTGAACCAGCGT CGCCGCCGTTCCATCCGCCGACGGGATGGCACCTTCAACTACAGTCCAGATGTCTACTGCACGAAGTATG ACGAGGCTACAGGCCTCTGCCCCGAGGGCGACGAGTGCCCATTCCTGCACAGAACCACTGGGGACACTGA GCGCAGGTATCACCTTCGTTACTATAAAACTGGAATCTGCATCCATGAGACAGACTCAAAAGGCAACTGC ACCAAAAATGGCCTGCACTGCGCTTTTGCCCACGGGCCTCATGACCTCCGCTCCCCTGTTTACGACATCA GGGAGCTCCAGGCCATGGAGGCCTTGCAGAATGGCCAGACTACAGTAGAGGGCAGCATAGAAGGCCAGTC AGCTGGGGCTGCAAGCCATGCCATGATAGAAAAAATCCTCAGTGAGGAACCCCGGTGGCAAGAGACTGCT TATGTGCTGGGGAACTATAAGACGGAGCCGTGCAAGAAGCCTCCACGGCTGTGCCGCCAGGGCTATGCCT GTCCCTACTACCACAACAGCAAGGACCGGCGTCGGAGTCCCCGGAAGCACAAATACAGGTCGTCTCCGTG TCCAAACGTCAAGCATGGGGATGAGTGGGGAGACCCTGGCAAGTGTGAAAACGGAGACGCCTGCCAGTAT TGCCACACACGCACAGAGCAGCAGTTCCACCCAGAGATCTACAAGTCCACCAAGTGTAATGACATGCAGC AGGCGGGCAGCTGCCCCCGAGGACCTTTCTGCGCCTTCGCCCACATAGAACCGCCACCCTTAAGTGATGA TGTGCAGCCTTCCTCAGCTGTGTCCAGCCCTACCCAGCCAGGTCCCGTCTTGTACATGCCATCTGCCGCT GGAGACTCGGTCCCTCTCCTCTGTAGGAATAGTGGCCTGGGCAGTCCGTCTCACCTCTGCAGCTCCCCAC CAGGCCCCAGCAGGAAGGCCTCAAACCTGGAGGGTCTGGTCTTCCCTGGGGAGTCCAGCCTTGCCCCTGG CAGCTACAAGAAAGCTCCTGGCTTCGAAAGGGAAGACCAGGTTGGGGCAGAGTACCTGAAGAATTTCAAG TGCCAGGCTAAATTAAAACCCCATTCGCTAGAGCCCAGGAGTCAAGAGCAGCCTCTGCTTCAACCCAAAC AGGACGTGCTGGGCATCCTCCCTGTGGGCAGCCCCCTGACTTCCAGCATCTCTTCCAGTATTACCTCCAG CTTGGCAGCCACTCCCCCCAGCCCTGCAGGCACCAACAGCACCCCTGGCATGAATGCAAATGCTCTGCCC TTCTACCCAACCAGCGACACGGTAGAGTCGGTCATAGAGTCTGCCTTGGATGACCTGGACCTGAATGAGT TTGGAGTGGCTGCCCTAGAGAAGACTTTTGATAACAGCGCAGTGCCCCACCCCAGCAGCGTCACAATCGG TGGCAGTTTGTTGCAGAGCTCCGCGCCTGTGAACATCCCTGGCTCCCTGGGCAGCTCAGCTTCCTTCCAC TCTGCTTCTCCATCCCCTCCCGTCAGCCTCTCCTCACACTTCCTGCAGCAGCCCCAGGGCCACTTGAGTC AGTCAGAAAACACGTTTTTGGGGACCTCAGCATCACATGGATCTTTGGGTCTGAACGGGATGAACAGCAG CATCTGGGAGCATTTTGCCTCTGGAAGCTTCTCCCCAGGCACTTCCCCTGCCTTCCTATCAGGGCCAGGG GCTGCTGAGCTGGCCCGGCTTCGGCAAGAGCTAGATGAAGCTAACGGCACCATCAAGCAGTGGGAGGAGT CCTGGAAGCAGGCCAAGCAGGCTTGTGATGCCTGGAAGAAGGAAGCGGAGGAGGCTGGTGAGCGGGCCAG TGCAGCCGGAGCAGAGTGTGAGCTGGCCCGGGAGCAGCGGGATGCCCTGGAGCTGCGGGTGAAGAAGCTG CAGGAGGAGCTGGAGCGGCTGCACACGGTGCCAGAAGCCCAGACCCTGCCAGCCGCCCCTGACCTGGAGG CGCTGTCCCTCTCCACCCTGTACTCCATCCAGAAGCAGCTGCGGGTCCACCTGGAACAAGTGGACAAGGC TGTGTTCCACATGCAGTCAGTGAAATGCCTTAAGTGTCAGGAGCAGACCCGGGCAGTGCTGCCGTGCCAA CACGCTGTTCTGTGTGAGCTCTGTGCTGAGGGCAGTGAGTGCCCCGTCTGCCAGCCCAGCCGGGCCCATG CCCTCCAGTCGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286006
Insert Size	2394 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286006.1, NP_001272935.1
RefSeq Size	3724 bp
RefSeq ORF	2394 bp
Locus ID	217331
Gene Summary	Sequence-specific RNA-binding protein which plays an important role in the establishment and maintenance of the early morphology of cortical neurons during embryonic development. Acts as a translation repressor and controls a translationally regulated cell morphology program to ensure proper structuring of the nervous system. Translational control depends on recognition of its binding element within target mRNAs which consists of a mandatory UAG trimer upstream of a U/A-rich motif. Associated with polysomes (PubMed:25737280).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.

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