Prdm4 (NM_001302886) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1700031E19Rik; 2810470D21Rik; AW552272; SC; SC-; SC-1; SC1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228951 representing NM_001302886 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGACATGAACTTGAGCCCAGTGGGGATGGAGCAGCTGTCTTCATCCTCTGTGAGCAATGCCCTGC CAGTCTCAGGAAGTCACCTGGGCTTGGCTGCCTCTCCCTCTCACAGTGCCATCCCTGCCCCAGGACTTCC AGTGGCAATTCCAAACCTGGGTCCCTCCCTGAGCTCTCTGCCTTCTGCCTTGTCTCTTATGCTCCCCGTG GGCATTGGAGACCGAGGAGTGATGTGTGGGCTACCTGAGAGGAACTACACCCTACCTCCACCTCCTTACC CTCACCTAGAGAGCAGTTACTTCAGAACCATTCTACCTGGCATTTTATCTTATTTAGCTGACAGACCACC TCCTCAGTATATCCACCCCAACTCTATAAATGTTGATGGAAATACAGCATTATCTATCACCAACAACCCT TCAGCACTAGATCCGTATCAGGCCAATGGAAATGTTGGATTAGAACTAGGCATTGTTTCAATAGACTCTC GCTCTGTAAACACACATGGTGCCCAAAGTCTTCATCCTAATGATGGCCACGAAGTAGCATTGGACACAAC AATCACCATGGAGAATGTTTCTAGGGTTACCAGCCCAATCTCCACAGATGGAATGGCAGAGGAGCTTACA ATGGACGGTGTTACAGGAGAGCATCCCCAAATCCCAAATGGCTCCAGAAGTCATGAGCCTCTCTCTGTGG ATTCTGTGAGCAACAGCCTTACAGCAGAAGCTGTAGGACATGGTGGTGTGATACCCATTCACGGGAATGG TCTGGAGCTTCCTGTGGTCATGGAAACTGACCACATTGCAAACCGGGTCAATGGGATGTCTGACAGTACC CTCAGTGACTCCATCCACACCGTGGCCATGAGCACCAACTCTGTAAGCGTGGCACTCTCTACCTCACACA ACCTGGCCTCCCTAGAGTCTGTTTCCCTCCATGAAGTTGGCCTTAGCCTAGAGCCTGTGGCTGTCTCTTC TATCACGCAGGAGGTTGCCATGGGGACAGGTCATGTAGATGTGTCCTCAGACAGCTTGTCTTTTGTACCA TCTTCACTGCAAATGGAAGACTCCAATTCAAACAAGGAAAACATGGCAACCTTGTTTACAATTTGGTGCA CTCTTTGTGACCGAGCCTACCCCTCAGATTGCCCTGATCATGGACCAGTGACTTTTGTTCCTGACACACC AATAGAGAGCAGAGCAAGACTATCCCTCCCGAAGCAGCTTGTTCTCCGCCAGTCCATCGTGGGAACAGAT GTTGGTGTATGGACTGCAGAAACCATTCCTGTGCGGACTTGCTTCGGACCTCTAATTGGTCAGCAGAGTC ACTCCTTGGAAGTAGCAGAGTGGACAGACAAGGCAGTTAACCATGTCTGGAAGATATACCACACTGGTGT CCTGGAGTTCTGTATCATTACAACCGATGAGAATGAGTGTAATTGGATGATGTTTGTGCGCAAAGCCAGG AACCGTGAAGAACAGAATTTGGTGGCCTATCCCCATGATGGGAAAATCTATTTCTGTACCTCACAAGACA TCCCCCCTGAAAGTGAGCTGCTTTTCTATTACAGCCGGAATTACGCTCAACAGATAGGTGTTCCTGAACA CCCAGATGTGCACCTCTGTAACTGTGGCAAGGAGTGCAGTTCCTATTCAGAGTTCAAAGCTCATCTGACC AGCCATATCCACAATCATCTCCCTAGCCAGGGCCACAGCAGCAGCCATGGGCCAAGCCACAGCAAGGAAA GGAAGTGGAAGTGTTCAATGTGCCCCCAGGCTTTTATCTCGCCTTCCAAACTCCACGTTCACTTTATGGG CCACATGGGTATGAAGCCCCACAAGTGTGATTTTTGTAGCAAGGCTTTTAGTGATCCAAGCAACCTACGG ACACACCTCAAAATACATACAGGTCAGAAAAATTATAGGTGTACTTTGTGTGACAAGTCTTTCACGCAGA AGGCTCACCTGGAGTCTCACATGGTCATCCACACGGGTGAGAAGAATCTCAAGTGTGATTACTGTGACAA GCTGTTCATGCGGAGACAGGACCTCAAGCAGCACGTGCTCATCCACACGCAAGAACGCCAGATCAAGTGT CCGAAGTGTGATAAACTGTTCTTGAGAACAAACCACTTGAAGAAGCATCTCAATTCTCACGAAGGAAAAC GAGATTATGTCTGCGAAAAATGTACAAAGGCTTATCTAACCAAGTACCATCTCACTCGCCACCTGAAGAC CTGCAAAGAGCCCAGCTCCAGCTCCTCTGCACAGGAGGAAGAGGATGATGAGTCGGAGGAAGAAGATCTC GCAGACTCCATGAGGACAGAGGACTGCAGGATGGGCAGTGCTGTTTACTCAACAGACGAGTCTCTCTCCG CACATAAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302886
Insert Size	2391 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302886.1, NP_001289815.1
RefSeq Size	3955 bp
RefSeq ORF	2391 bp
Locus ID	72843
Gene Summary	This gene encodes a member of the PR/SET family of zinc finger proteins. This protein has been shown to bind DNA in a sequence-specific manner and has been implicated in neural stem cell proliferation and differentiation. Pseudogenes have been identified on chromosomes 14 and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014] Transcript Variant: This variant (2) contains a longer 5' UTR and uses an alternate in-frame splice site in the central coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.

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