Wwp1 (NM_001276292) Mouse Untagged Clone
CAT#: MC228934
Wwp1 (untagged) - Mouse WW domain containing E3 ubiquitin protein ligase 1 (Wwp1), transcript variant 2
CNY 7,510.00
货期*
4周
规格
Product images
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经常一起买 (3)
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 8030445B08Rik; AIP5; SDRP1; Tiul1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC228934 representing NM_001276292
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGACGAGCAGCTGATTGTAAATGTGACCCCACAGACCACCTTGGAGTTCCGAGTTTGGAGTCACCAC ACTTTAAAGGCAGATGCTCTATTAGGAAAAGCAACAGTGGACTTGAAACAAGTGCTGTTAACACACAACA GAAAATGTTGCCTGTTGAAGGTACTATTGGAATTGATAATCATGTATCAACCAATACTGTTGTTCCCAAC TCCTGCTGTTCACATGTAGTTAATGGAGAAAACACACCTTCATCCCCGTCTCAGGTTGCTGCCAGACCCA AAAATGCTCCAGCTCCAAAGCCAGTCACATCTGCGCCCACCAGTGACACGGTTAATGGAGAATCATCATC TGTCCTAGCTGATAATACTTCAACCATGGGTACTTTACTACCATCTGAAGATACCACCTCGACTTCCAAT TGCACTAGCACCACCACCCAAGAGCCTCCTGTACAAGAACCCCCAGCATCCTCAGAACACAGTGAATGTA TTCCCTCTGCCAGTGCGGAAGTGGGACCAGAAGCTCGGAGCCTCATAGACCCTGACTCTGATTCTAGAAA TAATTCTGTGTTTGACAAAGTAAGGCAGCCAGAAGGGTGTGTAGAACCTCTGCGGCCACAGTCTGGAAAC ACCAACACAGAAGCTTTGCCATCAGGGTGGGAACAGAGAAAAGATCCTCATGGTAGAACCTATTATGTGG ATCATAATACTCGAACTACTACATGGGAGAGACCACAGCCTTTGCCTCCAGGTTGGGAAAGAAGAGTTGA TGATCGTGGAAGAGTTTATTATGTGGATCATAACACCAGAACAACAACCTGGCAGCGGCCTACTATGGAA TCTGTTCGAAATTTTGAACAGTGGCAGTCTCAGCGGAATCAATTGCAGGGAGCTATGCAACAATTCAACC AACGATACCTCTATTCGGCTTCCATGTTAGCTGCAGAAAATGACCCCTATGGACCCTTACCACCGGGCTG GGAAAAAAGAGTGGATTCAACAGACAGAGTTTACTTTGTGAATCACAATACAAAAACAACCCAGTGGGAA GATCCAAGAACTCAAGGCTTACCAAATGAAGAACCCCTGCCCGAAGGCTGGGAAATCAGGTATACTCGGG AAGGTGTTAGGTACTTTGTTGATCATAACACGAGAACAACAACATTCAAAGACCCTCGCAATGGGAAGTC ATCAGTAACTAAAGGTGGTCCACAGATTGCTTATGAACGCAGCTTTAGGTGGAAGCTTGCTCACTTCCGT TACTTGTGCCAGTCTAATGCACTACCGAGTCATGTAAAGATCAATGTGTCCCGACAGACATTATTTGAAG ATTCCTTCCAACAGATTATGGCATTAAAACCATATGACTTGAGGAGGCGATTATATGTAATATTTAGAGG AGAAGAAGGTCTTGATTATGGTGGCCTTGCAAGAGAATGGTTTTTCTTGCTTTCCCATGAAGTTTTGAAC CCAATGTATTGCTTATTTGAGTATGCAGGCAAGAATAACTATTGTTTACAGATAAATCCAGCATCAACTA TAAATCCTGACCATCTTTCATACTTCTGTTTTATTGGTCGCTTTATTGCAATGGCACTGTTCCATGGAAA GTTTATTGATACTGGTTTCTCTTTACCATTCTACAAGCGTATGCTAAGTAAAAAACTTACTATTAAGGAT TTGGAATCTATTGATACTGAATTTTACAACTCTCTAATCTGGATAAGAGATAACAATATTGAAGAATGTG GCTTAGAAATGTACTTTTCTGTAGACATGGAGATTCTGGGAAAAGTTACTTCACATGATTTAAAGTTGGG AGGTTCCAATATCCTGGTGACGGAAGAAAACAAAGATGAATATATTGGTTTAATGACAGAATGGCGTTTT TCTCGAGGAGTACAAGAACAGACCAAAGCTTTCCTTGATGGGTTTAATGAAGTTGTTCCCCTTCAGTGGC TACAGTACTTTGATGAAAAAGAATTGGAGGTTATGCTGTGTGGCATGCAAGAGGTTGACTTGGCAGACTG GCAGAGAAATACTGTTTATCGACATTATACAAGAAACAGCAAGCAAATTATTTGGTTTTGGCAGTTTGTG AAAGAGACTGATAATGAAGTACGAATGCGACTCTTGCAGTTTGTGACTGGAACCTGTCGCTTGCCTCTAG GAGGATTTGCTGAGCTCATGGGAAGTAATGGGCCTCAAAAGTTCTGTATTGAAAAAGTTGGCAAAGACAC CTGGTTACCAAGAAGCCATACATGTTTTAATCGCTTGGATCTACCACCATATAAGAGTTATGAACAACTA AAGGAAAAACTTCTTTTTGCAATAGAAGAGACAGAGGGATTTGGACAAGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001276292 |
Insert Size | 2364 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001276292.1, NP_001263221.1 |
RefSeq Size | 6296 bp |
RefSeq ORF | 2364 bp |
Locus ID | 107568 |
UniProt ID | Q8BZZ3 |
Gene Summary | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Ubiquitinates and promotes degradation of SMAD2 in response to TGF-beta signaling, which requires interaction with TGIF (By similarity). Ubiquitinates ERBB4 isoforms JM-A CYT-1 and JM-B CYT-1, KLF2, KLF5 and TP63 and promotes their proteasomal degradation. Ubiquitinates RNF11 without targeting it for degradation. Ubiquitinates and promotes degradation of TGFBR1; the ubiquitination is enhanced by SMAD7. Ubiquitinates SMAD6 and SMAD7.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has multiple coding region differences and initiates translation at a downstream start codon, compared to variant 1. The use of the start codon used in variant 1 would render the transcript a candidate for nonsense-mediated mRNA decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG, which is associated with a strong Kozak sequence. The resulting isoform (2) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR231145 | Wwp1 (myc-DDK-tagged) - Mouse WW domain containing E3 ubiquitin protein ligase 1 (Wwp1), transcript variant 2 |
CNY 7,890.00 |
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