Atp6v0a1 (NM_001243049) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AA959968; ATP6a1; Atp6n1; Atp6n1a; Atpv0a1; Vpp-1; Vpp1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228922 representing NM_001243049 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCGAAAACTCCGATTTGTTGAGAAAGAGATAAGGAAAGCTAACATCCCAATTATGGACACCGGTG AAAACCCAGAGGTGCCCTTCCCTCGGGACATGATCGACTTAGAGGCCAATTTCGAGAAGATTGAAAATGA ACTGAAGGAAATCAACACTAACCAGGAAGCTCTGAAGAGAAACTTCCTGGAACTGACTGAATTAAAATTT ATCCTGCGAAAAACCCAGCAGTTTTTCGATGAGCAGATGGCGGATCCAGACCTGTTGGAAGAGTCCTCAT CACTCTTGGAGCCAAACGAGATGGGAAGAGGCGCACCCTTACGACTTGGCTTTGTGGCTGGTGTGATTAA CCGGGAGCGGATCCCGACCTTTGAGCGCATGCTTTGGCGAGTGTGCCGAGGGAATGTGTTCCTGAGACAA GCTGAAATCGAAAACCCGCTGGAGGATCCTGTGACTGGCGACTACGTGCACAAGTCTGTGTTCATCATTT TTTTCCAAGGCGATCAGCTGAAAAACAGGGTCAAGAAAATCTGTGAAGGGTTCCGAGCCTCCCTCTATCC CTGCCCTGAGACTCCACAGGAAAGGAAAGAAATGGCTTCCGGAGTCAATACCAGGATCGACGACCTCCAA ATGGTTCTGAATCAGACGGAGGACCACCGCCAGAGGGTTCTGCAGGCAGCTGCTAAGAACATCCGCGTCT GGTTCATCAAGGTGCGGAAGATGAAGGCTATCTACCATACCCTGAACCTGTGCAATATAGACGTGACCCA GAAGTGCCTGATCGCAGAAGTCTGGTGCCCTGTCACTGACCTGGACTCCATCCAGTTTGCACTACGAAGA GGCACGGAACACAGTGGGTCCACTGTCCCCTCCATTCTGAACAGGATGCAGACAAACCAGACACCGCCGA CATATAATAAAACCAACAAGTTCACGCACGGCTTCCAGAACATAGTAGATGCTTATGGGATCGGCACTTA CCGAGAGATTAACCCAGCTCCGTACACTGTCATCACCTTCCCCTTTCTGTTTGCTGTGATGTTCGGAGAT TTTGGGCACGGCATTTTGATGACGCTGTTTGCTGTGTGGATGGTGTTGAGGGAGAGCCGGATCCTCTCCC AGAAGCACGAGAATGAGATGTTTAGCATGGTGTTCAGCGGCCGGTACATTATTCTTCTGATGGGACTGTT CTCCATCTATACTGGACTCATCTACAATGACTGCTTTTCCAAGTCTCTGAATATCTTTGGGTCATCGTGG AGTGTACGGCCAATGTTCACTCAAGGGAACTGGACGGAGGAGACGCTTCTGGGGAGTTCTGTTCTCCAGT TGAACCCCGCTATCCCTGGAGTTTTTGGTGGCCCGTACCCGTTTGGCATTGATCCGATTTGGAACATTGC AACCAACAAGCTGACCTTCCTCAACTCCTTCAAGATGAAGATGTCTGTTATCCTCGGCATCATCCACATG CTGTTTGGAGTCAGCCTGAGCCTTTTCAACCATATCTATTTCAAGAAGCCCCTGAACATCTACTTTGGCT TTATTCCTGAGATCATCTTCATGTCCTCGTTGTTTGGCTACCTGGTCATCCTTATCTTTTACAAGTGGAC AGCCTACGATGCCCACTCGTCTAGGAATGCCCCGAGCCTCCTGATCCACTTCATAAACATGTTCCTCTTC TCCTACCCAGAGTCTGGTAATGCAATGCTGTACTCTGGACAGAAAGGAATTCAGTGTTTCCTCATCGTGG TGGCGATGCTCTGCGTCCCTTGGATGCTTCTGTTTAAGCCGCTGATCCTTCGCCATCAGTACCTGAGGAA GAAGCATTTGGGAACTCTCAACTTTGGTGGGATCAGGGTGGGCAACGGACCGACAGAGGAGGATGCTGAA ATTATTCAGCATGACCAGCTTTCCACCCATTCAGAGGACGCAGAAGAGCCTACCGAGGACGAAGTGTTTG ACTTTGGCGACACCATGGTCCACCAGGCCATCCACACCATTGAGTACTGCTTGGGTTGTATCTCCAACAC CGCGTCGTACCTGCGACTCTGGGCCCTCAGCCTGGCCCACGCACAGCTCTCTGAAGTACTTTGGACCATG GTGATCCACATTGGCCTGCACGTCCGGAGTTTGGCCGGGGGTCTAGGGCTGTTTTTCATCTTCGCCGCCT TTGCCACGCTGACCGTGGCTATCCTGCTGATCATGGAGGGCCTCTCGGCCTTCCTCCACGCGCTGCGGTT ACACTGGGTGGAGTTCCAGAACAAATTCTACACTGGGACTGGCTTCAAGTTCCTCCCCTTCTCCTTTGAG CACATTCGGGAAGGGAAGTTTGATGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001243049
Insert Size	2340 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001243049.1, NP_001229978.1
RefSeq Size	3841 bp
RefSeq ORF	2340 bp
Locus ID	11975
Gene Summary	Required for assembly and activity of the vacuolar ATPase. Potential role in differential targeting and regulation of the enzyme for a specific organelle (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.