Cyld (NM_001276279) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2010013M14Rik; 2900009M21Rik; C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228906 representing NM_001276279 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCAGGCCTGTGGAGCCAAGAGAAAGTTACTTCACCCTACTGGGAAGAACGGATTTTTTATCTGC TTCTTCAAGAATGCAGTGTAACAGACAAACAAACTCAGAAGCTGCTGAAAGTACCCAAAGGGAGTATAGG ACAGTACATCCAAGACCGTTCTGTGGGGCATTCAAGAGTTCCTTCCACAAAAGGCAAGAAAAATCAGATT GGATTAAAAATCTTGGAGCAACCGCATGCAGTTCTGTTTGTTGATGAAAAGGATGTTGTAGAAATAAATG AAAAATTCACAGAGTTACTGTTGGCAATTACCAACTGTGAGGAGAGGCTCAGCCTATTTAGAAACAGACT CCGACTAAGTAAAGGCCTCCAGGTAGACGTGGGCAGTCCTGTGAAAGTACAGCTGCGATCTGGGGAAGAG AAATTTCCAGGAGTTGTACGCTTCAGAGGACCTTTATTAGCGGAGAGGACGGTGTCGGGGATTTTCTTTG GAGTAGAATTATTGGAAGAAGGTCGTGGTCAAGGTTTCACGGATGGGGTATATCAAGGGAAGCAGCTTTT CCAGTGTGATGAGGACTGTGGCGTGTTTGTTGCATTGGACAAACTAGAACTTATAGAAGATGATGACAAT GGATTGGAAAGTGATTTTGCAGGCCCAGGAGATACAATGCAGGTTGAACCTCCCCCTTTGGAAATAAACT CCAGAGTTTCTTTGAAGGTTGGAGAAAGTACAGAATCTGGAACAGTAATATTCTGTGATGTTTTACCAGG AAAAGAGAGTCTAGGATATTTTGTTGGTGTGGACATGGATAACCCTATTGGCAACTGGGATGGAAGGTTT GATGGAGTACAGCTCTGTAGTTTTGCAAGTGTTGAAAGTACAATTCTCCTGCACATCAATGACATCATCC CAGATAGCGTGACACAGGAAAGGAGGCCTCCCAAACTTGCCTTTATGTCAAGAGGTGTAGGTGACAAAGG TTCATCTAGTCATAATAAACCAAAGGTTACAGGATCTACCTCAGACCCTGGAAGTAGAAACAGATCTGAA TTATTTTATACCTTAAATGGGTCATCTGTTGACTCACAACAATCCAAGTCCAAAAATCCATGGTACATTG ATGAAGCATTTGGGGGCTATTTAAGTGAAGTAGTAGAAGAAAATACTCCACCTAAAATGGAAAAGGAAGG TTTAGAGATAATGATTGGAAAGAAGAAAGGCATCCAGGGCCATTACAATTCTTGTTACTTAGACTCAACT TTATTCTGCTTATTTGCTTTTAGTTCTGCCCTGGACACTGTGTTACTTAGACCCAAAGAGAAGAATGATA TAGAGTATTACAGTGAGACTCAGGAGCTACTGAGGACAGAGATAGTCAATCCTCTGAGAATATATGGATA TGTGTGTGCCACAAAGATTATGAAACTGAGGAAAATACTTGAAAAAGTTGAGGCTGCATCAGGTTTTACC TCTGAAGAAAAAGATCCTGAAGAATTCTTAAATATCCTGTTTCATGATATTTTAAGGGTTGAACCATTGT TAAAAATAAGATCAGCAGGTCAAAAAGTTCAAGACTGTAACTTCTATCAAATTTTTATGGAAAAAAATGA GAAAGTTGGAGTACCCACAATTCAGCAGTTATTAGAATGGTCTTTTATCAACAGCAACCTGAAATTTGCA GAGGCACCATCATGCTTGATTATCCAGATGCCTCGGTTTGGAAAAGACTTTAAACTATTTAAAAAAATTT TTCCTTCCCTGGAATTAAATATAACAGATTTACTTGAAGACACTCCCAGGCAGTGCCGCATCTGTGGAGG ACTTGCGATGTACGAGTGCAGGGAGTGCTATGACGATCCGGACATCTCAGCTGGGAAGATCAAGCAGTTC TGTAAGACCTGCAGCACTCAGGTTCACCTTCATCCCAGGAGGTTGAATCATTCTTATCATCCAGTATCAC TTCCCAAAGACTTGCCTGACTGGGACTGGAGACACGGCTGCATCCCCTGTCAGAAGATGGAGTTATTTGC TGTTCTCTGCATAGAAACGAGCCACTATGTTGCTTTTGTGAAGTATGGGAAGGATGACTCTGCCTGGCTT TTCTTTGACAGCATGGCGGATCGAGATGGTGGTCAGAATGGCTTCAACATTCCACAAGTGACGCCCTGCC CAGAAGTGGGAGAGTACTTGAAGATGTCTCTGGAGGACCTGCACTCTTTGGATTCCAGAAGGATTCAAGG CTGTGCGCGCAGACTTCTTTGCGATGCATACATGTGCATGTACCAGAGTCCAACCATGAGCCTGTACAAA TAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001276279
Insert Size	2313 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001276279.1, NP_001263208.1
RefSeq Size	7599 bp
RefSeq ORF	2313 bp
Locus ID	74256
Gene Summary	This gene encodes a protein that is a member of the ubiquitin C-terminal hydrolase subfamily of the deubiquitinating enzyme family. Members of this family catalyze the removal of ubiquitin from a substrate or another ubiquitin molecule and thereby play important roles in regulating signaling pathways, recycling ubiquitin and regulating protein stability. This protein removes ubiquitin from K-63-linked ubiquitin chains from proteins involved in NF-kappaB signaling and thus acts as a negative regulator of this pathway. In humans mutations in this gene have been associated with cylindromatosis, an autosomal dominant predisposition to tumors of skin appendages. In mouse deficiency of this gene impairs thymocyte development and increases susceptibility to skin and colon tumors. A pseudogene of this gene has been identified on chromosome 1. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013] Transcript Variant: This variant (5, also known as short (s)-CYLD) differs in the 5' UTR and lacks several alternate exons in the coding region compared to variant 2. It encodes isoform c which is shorter than isoform b. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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