Pla2g6 (NM_001199024) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	BB112799; iPLA(2)beta; iPLA2; iPLA2beta; PNPLA9
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228857 representing NM_001199024 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTTCTTCGGACGCCTCGTCAACACCCTCAGTAGCGTCACCAACTTGTTCTCGAACCCATTCCGGG TGAAGGAGGTGTCCCTGACTGACTACGTCTCAAGTGAACGTGTCCGGGAGGAAGGGCAGCTGATCCTGTT ACAGAATGTCTCCAATCGCACCTGGGACTGTGTCCTGGTCAGCCCGAGGAACCCACAGAGCGGCTTCCGG CTCTTCCAACTGGAGTCTGAGGCAGACGCCCTGGTGAACTTCCAGCAGTTCTCCTCCCAGCTGCCGCCCT TCTACGAGAGCTCTGTGCAGGTCCTGCATGTGGAGGTGCTTCAGCACCTGACCGACCTCATCCGGAACCA CCCCAGCTGGACAGTGACACACCTAGCCGTGGAGCTCGGCATCCGGGAGTGCTTCCATCACAGCCGCATC ATCAGCTGTGCCAACAGCACAGAGAATGAGGAGGGCTGCACCCCACTACATCTGGCCTGCCGCAAGGGTG ACAGTGAGATCCTGGTGGAGCTGGTACAATACTGCCACGCCCAGATGGATGTCACTGACAACAAAGGAGA GACTGCCTTCCATTACGCTGTGCAAGGGGACAATCCCCAGGTGCTACAGCTCCTAGGGAAGAACGCCTCA GCCGGCCTGAACCAGGTAAACAACCAAGGGCTGACTCCACTGCACCTGGCCTGCAAGATGGGAAAGCAGG AGATGGTGCGCGTCCTGCTGCTCTGTAATGCCCGCTGCAACATCATGGGGCCCGGTGGCTTCCCCATCCA CACAGCCATGAAGTTTTCCCAGAAGGGGTGTGCTGAAATGATTATCAGCATGGACAGCAACCAGATCCAC AGCAAGGATCCTCGCTACGGAGCCAGCCCACTCCATTGGGCCAAGAACGCCGAGATGGCCCGAATGCTGC TGAAGCGGGGCTGTGACGTGGACAGCACTAGCTCCTCAGGGAACACAGCCCTGCATGTGGCGGTGATGCG CAACCGCTTTGACTGTGTCATGGTGCTGCTGACCTACGGGGCTAATGCAGGTGCCCGCGGAGAGCACGGG AACACGCCACTGCACCTGGCCATGTCGAAAGATAACATGGAGATGGTCAAAGCCCTCATTGTATTTGGGG CAGAAGTGGACACCCCAAACGACTTTGGGGAGACTCCTGCATTGATAGCCTCCAAGATCAGCAAGCAGCT TCAGGATCTCATGCCCATCTCTCGAGCCCGGAAGCCAGCGTTCATCCTGAGCTCCATGAGGGACGAGAAG CGGAGTCACGACCACCTGCTCTGCCTGGACGGAGGGGGCGTGAAAGGCCTGGTCATTATCCAGCTTCTCA TCGCCATCGAGAAGGCCTCGGGAGTGGCCACCAAGGACCTCTTCGACTGGGTGGCAGGAACCAGCACAGG GGGCATCCTGGCCCTGGCCATTCTGCACAGTAAATCCATGGCCTATATGCGTGGTGTGTACTTCCGTATG AAGGACGAGGTGTTTCGGGGCTCACGGCCCTATGAGTCTGGGCCCCTGGAGGAGTTCCTGAAGCGGGAGT TTGGGGAGCACACCAAGATGACAGATGTCAAAAAACCCAAGGTGATGCTGACAGGGACACTGTCTGACCG GCAGCCAGCAGAGCTCCACCTATTCCGGAATTACGATGCTCCCGAAGCCGTTCGAGAGCCCCGCTGCAAC CAAAACATTAACCTGAAGCCACCGACTCAGCCTGCAGACCAACTGGTATGGCGTGCAGCCCGGAGCAGTG GGGCAGCCCCAACCTATTTCCGGCCCAATGGACGCTTCCTGGATGGAGGGCTGCTGGCCAACAACCCCAC ACTGGATGCCATGACTGAAATCCATGAGTACAATCAGGACATGATCCGCAAGGGCCAGGGCAACAAGGTG AAGAAACTCTCCATAGTCGTTTCTCTGGGGACAGGAAAGTCCCCTCAAGTGCCTGTAACCTGTGTAGATG TCTTTCGTCCCAGCAACCCTTGGGAACTGGCCAAAACTGTTTTTGGAGCCAAGGAACTGGGCAAGATGGT CGTGGACTGTTGCACAGATCCAGATGGGCGGGCTGTGGATCGGGCCCGGGCCTGGTGCGAGATGGTCGGC ATCCAGTACTTCAGACTGAACCCCCAGCTAGGGTCCGACATCATGCTGGACGAGGTCAGTGATGCAGTGC TGGTCAACGCCCTCTGGGAGACCGAGGTCTACATCTATGAGCACCGAGAGGAGTTCCAGAAGCTTGTCCA GCTGCTGCTGTCTCCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001199024
Insert Size	2259 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199024.1, NP_001185953.1
RefSeq Size	3560 bp
RefSeq ORF	2259 bp
Locus ID	53357
UniProt ID	P97819
Gene Summary	Catalyzes the release of fatty acids from phospholipids. It has been implicated in normal phospholipid remodeling, nitric oxide-induced or vasopressin-induced arachidonic acid release and in leukotriene and prostaglandin production. May participate in fas mediated apoptosis and in regulating transmembrane ion flux in glucose-stimulated B-cells. Has a role in cardiolipin (CL) deacylation. Required for both speed and directionality of monocyte MCP1/CCL2-induced chemotaxis through regulation of F-actin polymerization at the pseudopods (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1, and encodes isoform (1). Variants 1, 3, and 4 encode the same protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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