Mme (NM_001289462) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	6030454K05Rik; C85356; CALLA; CD10; NEP; SFE
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228849 representing NM_001289462 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAAGATCAGAAAGTCAGATGGATATAACTGATATCAATGCTCCAAAGCCAAAGAAGAAACAGCGAT GGACTCCCCTGGAGATCAGCCTCTCTGTGCTTGTCTTGCTCCTGACTATCATAGCGGTGACAATGATCGC TCTCTATGCAACCTATGATGATGGCATTTGCAAATCATCAGACTGCATAAAATCAGCTGCTCGACTGATT CAGAATATGGATGCCTCTGTTGAGCCATGTACAGACTTCTTCAAGTATGCTTGTGGAGGCTGGTTGAAAC GCAACGTCATTCCCGAGACCAGTTCCCGATACAGTAATTTTGACATTTTAAGAGATGAGCTAGAAGTCAT TTTGAAAGATGTCCTTCAAGAACCCAAAACTGAAGACATAGTAGCAGTGCAGAAAGCAAAAACCTTGTAC AGATCGTGTATAAATGAGTCTGCTATTGATAGCAGAGGCGGACAACCTCTACTCAAACTGTTACCAGATA TATATGGGTGGCCAGTAGCATCAGATAACTGGGATCAAACATATGGCACTTCTTGGACAGCTGAGAAATC CATTGCACAACTGAATTCTAAATATGGGAAAAAGGTCCTCATTAATTTTTTTGTTGGCACTGATGATAAG AATTCTACCCAGCATATAATTCATTTTGACCAGCCTCGACTTGGCCTCCCTTCCAGAGACTACTATGAGT GCACTGGAATATATAAAGAGGCTTGCACAGCATATGTGGATTTTATGATTTCTGTGGCCAGACTGATTCG TCAGGAACAAAGTTTGCCTATTGATGAAAACCAGCTCTCTTTGGAAATGAATAAAGTTATGGAATTGGAA AAAGAAATTGCCAATGCTACAACTAAACCAGAAGACCGAAATGACCCAATGCTGCTTTATAACAAAATGA CATTGGCCAAGCTGCAGAACAACTTTTCCCTGGAGGTCAATGGGAAGTCATTCAGCTGGTCAAATTTCAC AAATGAAATCATGTCAACTGTGAATATTAATATTCAAAATGAGGAAGAAGTTGTTGTCTATGCTCCAGAA TATTTAACCAAACTTAAGCCTATTCTTACCAAATATTCTCCCAGAGATCTTCAAAATTTAATGTCCTGGA GGTTCATAATGGATCTTGTAAGCAGCCTCAGCCGAAACTACAAGGAGTCCAGAAATGCTTTCCGCAAGGC CCTTTATGGGACTACATCAGAAACTGCAACATGGAGACGGTGTGCTAACTACGTCAATGGGAATATGGAG AATGCTGTGGGGAGGCTTTATGTGGAAGCGGCTTTTGCTGGAGAGAGCAAGCACGTGGTTGAAGACTTGA TTGCACAAATCCGGGAAGTTTTTATTCAGACTTTGGATGACCTTACTTGGATGGATGCTGAGACAAAAAA GAAAGCTGAAGAAAAGGCCTTGGCAATTAAAGAAAGGATTGGCTATCCTGATGACATCATTTCCAATGAG AACAAACTGAATAATGAGTATCTTGAGTTGAACTACAGGGAAGATGAATACTTTGAGAACATAATTCAAA ATTTGAAATTCAGCCAAAGCAAGCAGCTAAAGAAGCTCCGAGAAAAAGTGGACAAAGATGAGTGGATAAG TGGAGCAGCTGTAGTCAATGCATTTTATTCCTCAGGCCGAAATCAGATAGTCTTCCCCGCTGGCATTTTG CAGCCCCCATTCTTCAGTGCTCAGCAATCCAACTCATTGAACTATGGGGGCATCGGCATGGTCATTGGAC ATGAAATCACACATGGATTTGATGACAATGGAAGAAATTTCAATAAAGATGGAGACCTCGTTGACTGGTG GACGCAGCAGTCGGCAAATAATTTTAAAGACCAATCCCAGTGTATGGTATACCAGTATGGAAACTTTTCC TGGGACCTAGCAGGTGGACAGCATCTCAATGGAATTAATACACTAGGAGAAAACATTGCTGATAATGGAG GTATTGGCCAAGCATACAGAGCCTATCAGAATTATGTTAAAAAGAATGGTGAAGAAAAATTACTCCCTGG ACTTGACCTCAATCACAAACAACTATTTTTCCTGAACTTTGCCCAGGTGTGGTGTGGGACCTACCGGCCA GAGTATGCAGTCAATTCTATTAAAACAGATGTACACAGTCCTGGAAATTTCAGGATCATTGGGACTTTGC AGAACTCTGCCGAGTTTGCAGATGCCTTTCATTGCCGCAAGAATTCATACATGAATCCAGAAAGGAAATG TCGGGTTTGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289462
Insert Size	2253 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289462.1, NP_001276391.1
RefSeq Size	6088 bp
RefSeq ORF	2253 bp
Locus ID	17380
UniProt ID	Q61391
Gene Summary	Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF) (By similarity). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573).[UniProtKB/Swiss-Prot Function]

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