Atg7 (NM_001253717) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228822 representing NM_001253717 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATACAGGCCTCTGGAAAATTCCCACGAGCACAGTGGTGAGGCCAACTCCACACTGCTTTCCTGCCA TGAGGCTTTCCTCATCTGGATGTGAGCACCTGACAGTGAGGCTGGTAAGAACAGTAGCCATGGGGGACCC TGGACTGGCCAAGTTGCAGTTCGCCCCCTTTAATAGTGCCCTGGACGTTGGCTTCTGGCACGAACTGACC CAGAAGAAGTTGAACGAGTACCGCCTGGACGAGGCACCCAAAGACATCAAGGGCTATTACTACAATGGTG ACTCTGCTGGTCTGCCCACCCGCTTGACGTTGGAGTTCAGTGCTTTTGACATGAGTGCCTCCACGCCTGC CCACTGCTGCCCGGCCATGGGAACCCTGCACAACACCAACACACTTGAGGCTTTTAAGACAGCAGACAAG AAGCTCCTTCTGGAGCAGTCAGCAAATGAGATCTGGGAAGCCATAAAGTCAGGTGCTGCTCTCGAAAACC CCATGCTCCTCAACAAGTTTCTGCTCCTGACCTTCGCGGACCTAAAGAAGTACCACTTCTACTACTGGTT TTGCTGCCCCGCCCTCTGTCTTCCTGAGAGCATCCCTCTAATCCGGGGACCTGTGAGCTTGGATCAAAGG CTTTCACCAAAACAGATCCAGGCCCTGGAGCATGCCTATGATGATCTGTGTCGAGCCGAAGGCGTCACGG CCCTGCCCTACTTCTTATTCAAGTACGATGACGACACTGTTCTGGTCTCCTTGCTCAAACACTACAGTGA TTTCTTCCAAGGTCAAAGGACAAAGATAACAGTTGGTGTGTACGATCCCTGTAACCTAGCCCAGTACCCT GGATGGCCTTTGAGGAATTTTTTGGTCCTGGCAGCCCACAGATGGAGCGGCAGTTTCCAGTCCGTTGAAG TCCTCTGCTTTCGGGACCGCACCATGCAGGGAGCTAGAGACGTGACACATAGCATCATCTTTGAAGTGAA ACTTCCAGAAATGGCATTTAGCCCAGATTGTCCTAAAGCTGTTGGCTGGGAGAAGAACCAGAAAGGAGGC ATGGGTCCGAGGATGGTGAACCTCAGTGGATGTATGGACCCCAAAAGGCTGGCTGAGTCATCTGTGGATC TGAATCTCAAGCTGATGTGCTGGCGATTGGTCCCCACCTTGGACTTGGACAAGGTCGTGTCTGTCAAGTG CCTGCTGCTGGGAGCTGGTACCTTGGGGTGTAATGTGGCTAGGACACTGATGGGCTGGGGCGTCAGACAT GTCACCTTTGTGGATAACGCCAAGATCTCCTACTCCAATCCCGTGAGGCAGCCTCTGTATGAATTTGAAG ATTGTCTAGGGGGTGGCAAGCCCAAGGCCCTGGCTGCAGCAGAGCGGCTACAGAAAATATTTCCCGGAGT GAATGCCAGAGGGTTCAACATGAGCATCCCCATGCCAGGACACCCTGTGAACTTCTCTGACGTCACGATG GAGCAGGCCCGCAGAGATGTGGAGCAGCTGGAGCAGCTCATTGATAACCATGATGTCATCTTCCTGCTAA TGGACACCAGGGAGAGCCGGTGGCTTCCTACTGTTATTGCAGCCAGCAAGCGAAAGCTGGTCATCAACGC TGCCTTGGGGTTTGATACCTTTGTTGTCATGAGACATGGCCTGAAGAAACCCAAGCAGCAGGGAGCCGGA GACCTCTGCCCAAGCCATCTTGTAGCACCTGCTGACCTGGGCTCCTCACTTTTTGCCAACATCCCTGGAT ACAAGCTTGGCTGCTACTTCTGCAATGATGTGGTGGCTCCAGGAGATTCAACCAGAGACCGGACTCTGGA CCAGCAGTGCACAGTGAGCCGCCCAGGCCTGGCCGTGATTGCAGGTGCCCTGGCTGTGGAGCTGATGGTC TCTGTCCTGCAGCATCCTGAGGGGGGCTACGCCATCGCCAGCAGCAGTGATGACCGCATGAATGAGCCTC CCACCTCGCTGGGACTTGTGCCTCACCAGATCCGGGGTTTTCTGTCACGGTTCGATAATGTTCTTCCTGT CAGCCTGGCATTTGATAAATGTACAGCCTGTTCACCCAAAGTTCTTGATCAGTACGAGCGAGAAGGATTC ACCTTCCTAGCGAAGGTTTTTAACTCCTCACATTCCTTCTTAGAAGACTTGACCGGTCTTACCCTGCTCC ATCAAGAGACCCAAGCTGCTGAGATCTGGGACATGAGTGACGAGGAGACTGTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001253717
Insert Size	2226 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001253717.1, NP_001240646.1
RefSeq Size	3925 bp
RefSeq ORF	2226 bp
Locus ID	74244
UniProt ID	Q9D906
Gene Summary	This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).

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