Map4k2 (NM_001291787) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI385662; BL44; GCK; Rab8ip
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228808 representing NM_001291787 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTCTGTGGAGGAGGGTCACTGCAGGAGATTTACCACGCCACTGGGCCCCTGGAAGAACGGCAGA TTGCCTATGTTTGCCGGGAGGCACTGAAGGGGCTCCACCACCTGCATTCTCAGGGGAAAATTCACCGAGA CATTAAGGGTGCCAACCTTCTGCTCACTCTGCAGGGTGATGTCAAGCTGGCGGACTTTGGGGTGTCAGGC GAGCTGACAGCATCTGTGGCCAAAAGACGTTCTTTCATTGGCACTCCATACTGGATGGCACCAGAGGTTG CCGCTGTGGAACGCAAGGGTGGCTACAATGAGCTGTGTGACGTCTGGGCCCTGGGCATCACTGCCATTGA GTTGGGCGAGCTGCAACCGCCACTGTTCCACCTGCACCCCATGAGGGCCTTGATGCTCATGTCGAAAAGC AGCTTCCAGCCACCCAAGCTAAGAGATAAGACGCGTTGGACCCAGAATTTCCACCACTTCCTCAAGCTGG CCCTAACCAAGAACCCCAAGAAGAGGCCTACAGCAGAGAGGCTTCTGCAGCACCCATTCACGACCCAGCA TCTCCCTCCGGCCCTCCTCACCCAGCTCCTCGACAAGGCCAGTGACCCCCACCTGGGAACCCTCTCCCCT GAAGACAGTGAGCTGGAGACCCATGACATGTTTCCAGATACCATCCATTCCCGCAGCCATCATGGTCCTG CTGAGAGGACCCCCTCCGAGATCCAGTTTCACCAAGTGAAATTCGGTGCTCCTCGCCGGAAGGAGACAGA TCCTCTCAATGAACCGTGGGAGGAGGAGTGGACGCTCCTGGGAAAAGAGGAACTGAGTGGGAGCCTGCTG CAGTCAGTCCAGGAGGCCCTGGAGGAAAGGAGCCTGACTATCCGGCCAGCCTTAGAACTCCAGGAGCTGG ACTCCCCGGATGATGCCATCGGAACCATCAAACGGGCTCCATTTTTGGGGCTGCCCCACACTGAGTCAAC ATCTGGTGACAATGCCCAGTCCTGCTCCCCAGGTACCCTGTCAGCACCTCCAGCTGGCCCTGGCAGCCCC GCATTGCTGCCTACCGCCTGGGCTACTCTGAAGCAACAGGAGGATCGTGAGCGATCATCCTGCCATGGGC TTCCTCCCACCCCCAAGGTGCACATGGGTGCCTGCTTCTCCAAGGTCTTCAATGGCTGCCCCCTGCAGAT CCATGCTGCTGTCACTTGGGTTCACCCTGTGACTCGAGACCAGTTCCTGGTGGTAGGGGCTGAGGAGGGC ATCTATACCCTCAACCTGCACGAACTGCACGAGGACACAATGGAGAAGCTCATCTCCCAGCGCTGCTCCT GGCTCTACTGTGTGAACAATGTGTTGCTGTCGCTCTCAGGGAAATCCACACATATCTGGGCGCATGACCT CCCGGGCCTGTTTGAGCAGCGAAGGCTGCAGCACCAGGCACCCCTCTCCATCCCTACCAACCGCATCACT CAGCGCATCATCCCCAGGCGCTTTGCCCTGTCCACCAAGATTCCTGACACCAAAGGCTGCTTGCAGTGCC GTGTGGTTCGAAACCCCTATACCGGCAGTACGTTCCTGCTGGCCGCCCTGCCTGCCAGCCTGCTTCTGCT GCAATGGTATGAGCCACTGCAGAAATTCCTGCTGCTGAAGAACTTTTCCAGTCCCTTGCCCAGCCCAGCG GGGATGCTGGAGCCGCTGGTGCTAGATGGGAAGGAGCTGCCACAGGTGTGTGTGGGCGCCGAGGGACCCG AGGGACCCGGCTGTCGAGTCCTGTTCCATGTCTTGCCCCTGGAAGCTGGCCTGACTCCAGACATCCTCAT CCCTCCTGAGGGGATCCCAGGTTCTGCACAGCAGGTGATCCAAGTGGACAGGGACACAGTCCTGGTCAGC TTTGAACGCTGTGTGAGAATTGTTAACCTGCAGGGGGAACCCACGGCTGCACTTGCCCCTGAACTAACCT TTGACTTCACTATTGAGACTGTGGTGTGTCTGCAGGATAGTGTGCTGGCGTTCTGGAGCCATGGCATGCA GGGCCGAAGCCTCGATACCAACGAGGTGACGCAGGAGATAACAGATGAGACCAGGATCTTCCGAGTACTG GGGGCTCACAGGGACATCATCCTGGAAAGCATCCCAACTGATAACCCCGGGGCACACAGCAACCTCTACA TCCTCACGGGTCACCAGAGCAGCTACTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001291787
Insert Size	2199 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291787.1, NP_001278716.1
RefSeq Size	4653 bp
RefSeq ORF	2199 bp
Locus ID	26412
UniProt ID	Q61161
Gene Summary	Serine/threonine-protein kinase which acts as an essential component of the MAP kinase signal transduction pathway (PubMed:8643544). Acts as a MAPK kinase kinase kinase (MAP4K) and is an upstream activator of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway and to a lesser extent of the p38 MAPKs signaling pathway (By similarity). Required for the efficient activation of JNKs by TRAF6-dependent stimuli, including pathogen-associated molecular patterns (PAMPs) such as polyinosine-polycytidine (poly(IC)), lipopolysaccharides (LPS), lipid A, peptidoglycan (PGN), or bacterial flagellin (By similarity). To a lesser degree, IL-1 and engagement of CD40 also stimulate MAP4K2-mediated JNKs activation (By similarity). The requirement for MAP4K2/GCK is most pronounced for LPS signaling, and extends to LPS stimulation of c-Jun phosphorylation and induction of IL-8 (By similarity). Enhances MAP3K1 oligomerization, which may relieve N-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation (By similarity). Mediates also the SAP/JNK signaling pathway and the p38 MAPKs signaling pathway through activation of the MAP3Ks MAP3K10/MLK2 and MAP3K11/MLK3 (By similarity). May play a role in the regulation of vesicle targeting or fusion (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1.

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