Rxfp2 (NM_001289564) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Gpr106; Great; Lgr8
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228748 representing NM_001289564 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCTCCTACTTCATGTCATCCTTCTGACAGAGGTCAAAGATTTTGCACTGGCTGACAGCAGTATGG TGGCTCCTCTGTGCCCCAAAGGGTATTTTCCCTGTGGGAATCTCACCAAATGCTTGCCCCGAGCCTTTCA CTGCGATGGTGTGGATGATTGCGGGAATGGTGCCGACGAGGACAACTGTGGTGACACTAGTGGATGGACA ACCATATTTGGCACAGTCCATGGGAATGTCAATAAAGTGACATTGACACAGGAGTGCTTTCTCAGCCAGT ATCCACAGCACTGTTACTGCAGAGAAAATGAACTGGAATGTGTAAAGGCTGACTTAAAAGCTGTGCCAAA GGTTTCCAGCAACGTAACATTACTATCTCTTAAGAAAAACAAAATCCACAGACTTCCAGTCAAGGTCTTC AGCAGATACACAGAACTCAGAAAGATATACCTTCAGCACAACTGCATCACACACATCTCCAGGAGAGCAT TCCTTGGATTACATAATCTACAAATACTGTATCTCAGCCATAACTGCATTACCTCTCTCAGGCCTGGGAT ATTCAAAGACTTGCATCAGCTTGCTTGGCTAATTTTAGATGACAACCCGATCACCAGAATCTCACAGAAG TCCTTTATGGGGTTAAACTCCTTGTTTTTCTTGTCCATGGTGGGTAACCGGCTCGAGGCCCTTCCTGAAA CATTGTGTGCTCAGATGCCTCAACTCAACTGGGTGGATCTGGCAAACAATGGAATAAAGTACATAACGAA CTCCACCTTCCTAACGTGCGACTCGCTCACGGTTCTGGACCTGTCTAGCAATATGATAACAAAACTCCCA GTCCACCTTTTCAGCGACCTTCATCTTCTCCAGAAGCTGAACCTGTCATCCAACCCTCTTCTGTATGTCC ACAAGAACCAGTTTGGAAGTCTCAAACAACTTCAGTCTCTAGACCTGGAAAGGATAGAGATTCCAAACAT AAGCACAGGAATGTTCCAGCCAATGAAGAACCTTTCTCACATTTATTTGAAAACCTTTCGATACTGCTCC TATGTCCCCCATGTCCGAATCTGTATGCCGTCGACTGATGGTATTTCTTCGTCTGAGGACCTCTTGGCTA ACGGTATCCTCAGAGTGTCTGTCTGGGTTATAGCTTTCATTACCTGCGTTGGGAATTTCCTTGTCATAGC CGTGAGATCTCTCATTAAGGCTGAGAATACAACTCACGCTATGTCCATCAAAATCCTTTGTTGTGCCGAT TGCCTGATGGGGGTGTACCTGTTCTCCGTGGGCGTCTTTGACATCAAGTACCGAGGGCAGTATCAGAAGT ATGCGCTGCTGTGGATGGAGAGTGTGCCCTGCCGCCTGCTGGGCTTCCTGGCCACGCTGTCCACAGAGGT CTCGGTGCTGCTGCTGACATTCCTGACGCTGGAGAAGTTCCTTGTCATAGTATTCCCTTTCAGCAACCTG CGCCTGGGCAAGCGCCAGACTGCTGTGGCCCTCGCCAGCATCTGGGTGGTGGGATTTCTCATAGCGGCCG TTCCGTTCACCAGAGAGGATTATTTCGGCAACTTTTATGGGAAAAATGGAGTCTGCTTCCCACTTCATTA TGACCAAGCAGAAGATTTTGGAAGTAGAGGGTACTCCCTTGGGATTTTCCTAGGTGTGAACTTGCTGGCT TTCCTCGTCATCGTGATTTCCTATGTCACCATGTTCTGCTCCATTCATAAAACAGCCCTTCAGACTGCAG AAGTGAGGAGCCACATCGGGAAGGAGGTGGCTGTTGCAAACCGGTTCTTTTTTATCGTGTTCTCTGATGC CATCTGCTGGATCCCTGTGTTTGTCGTTAAGATCCTGTCTCTCCTTCAAGTGGAGATACCAGGCACAATC ACTTCCTGGATCGTGGTTTTTTTCCTTCCGGTGAACAGCGCCTTAAACCCCATCCTCTACACTCTGACGA CCTCCTTTTTTAAGGACAAGTTGAAACAGTTGCTGCACAAACATCGGAGGAAACCCATCTTCAAAGTTAA GAAGAAAAGTTTATCCGCTTCCATTGTGTGGACAGACGAGTCTTCACTTAAACTTGGAGTGTTGAGCAAA ATAGCCCTTGGGGACAGTATAATGAAGCCGGTCTCCCCGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289564
Insert Size	2142 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289564.1, NP_001276493.1
RefSeq Size	3317 bp
RefSeq ORF	2142 bp
Locus ID	140498
UniProt ID	Q91ZZ5
Gene Summary	Receptor for relaxin. The activity of this receptor is mediated by G proteins leading to stimulation of adenylate cyclase and an increase of cAMP. May also be a receptor for Leydig insulin-like peptide (INSL3) (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

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