Zscan10 (NM_001289484) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	BC068284; Zfp206; Zkscan10; Znf206
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228728 representing NM_001289484 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGCGGAACCAGTCCCTGATGCCCTGGAACAAGAGCATCCCGGAGCAGTGAAGTTGGAGGAGGATG AAGTTGGCGAGGAGGATCCCAGGCTCGCAGAGTCCAGGCCTAGGCCTGAGGTGGCCCACCAGCTTTTCAG ATGCTTCCAGTATCAGGAAGATATGGGGCCACGGGCATCCCTGGGCCGGCTCCGGGAACTCTGCAACCAC TGGCTGCGACCGGCTCTGCACACCAAGAAGCAGATCCTGGAGCTGCTGGATTTTAGCTTCAGTGCTGGCA AGAATGCCCCTGCAGACATCATCTCAGAGGAACAAAATAGCCCTTCCCAGGTCCCCAGCCACAGCCCCCA GACGGAGTTGCCCTCAGAAGAGATTCCAGCCCTACATCCACTGAATGAGTTACCTCCACCTCAGCCAGCA CCCATAAGGCCTGCTGAGCCTGAGGAGTGGAGACTGGCCCCCAGTTCAAATTGGCCAATGAGCCCAGAGC CCCAGGAGATACTCCAGGACCCACGAGAAAGCAACCCTTCCCAGGGCCCTTCATGGCTTGAGGAAAATTC CAGAGACCAAGAGCTGGCGGCTGTGTTGGAGTCCCTCACCTTTGAGGATACCTCAGAGAAGAGAGCTTGG CCTGCAAACCCTCTTGGATTTGGAAGCAGAATGCCTGACAATGAGGAACTTAAAGTTGAAGAGCCTAAAG TGACTACTTGGCCTGTCGTCATTGGAGCAGAGTCCCAGACAGAGAAACCTGAAGTTGCAGGAGAGCCTCT TACGCAAACTGTAGGGCAGGAGACCAGCAGCACTGGTTGGGGAGGTACTCCTGCTGACGGCAGTGAAGTT GTGAAGGCCCACCAGTTACAATCTCACCCCAACTTGCAACCTCACCCAAGCTCTCGATCCTTCCGATGTC TGTGGTGTGGGAAGACTTTTGGACGCAGCTCGATCCTCAAGCTGCACATGCGCACTCACACAGACGAGCG GCCGCACGCCTGTCATCTCTGCAACCGCCGCTTCCGCCAGAGCTCACACCTGACGAAGCACTTGCTAACG CATTCCTCTGAGCCTGCCTTCCGATGCGCCGAGTGTAACCAGGGTTTTCAGCGTCGCTCCAGCCTCATGC AGCACCTGCTGGCACATGCCCAGGGAAAGAATCTCACGCCAAATCCAGAAGGCAAGACAAAAGTGCCAGA GATGGCAGCTGTCCTCTGTTCCCACTGCGGGCAGACCTTCAAGCGGCGCTCTAGCTTAAAGCGTCACCTG CGTAACCATGCCAAGGACAAGGACCATCTGTCCTCTGAAGACCCTGGCAGCCTTAGCTCTAGCCAGGAGA GTAACCCCTATGTGTGTAGTGACTGTGGCAAGGCCTTCCGACAAAGCGAGCAACTAATGATCCACACTAG GCGAGTCCATACCCGTGAACGACCCTTCTCCTGCCAGGTCTGTGGCCGCTGCTTTACCCAAAATTCCCAG CTGATCAGCCACCAGCAGATTCATACGGGTGAGAAGCCTCACGCCTGTCCTCAGTGCAGCAAACGCTTTG TGAGACGAGCTGGCCTTGCTCGGCATCTGTTGACCCACGGTAGCCTCCGGCCTTACCACTGTGCCCAATG TGGCAAAAGCTTTCGCCAAATGCGAGACCTAACCCGCCACGTACGCTGCCACACGGGGGAGAAGCCCTGC CGATGCAACGAATGTGGAGAGGGGTTCACCCAGAATGCCCACCTGGCACGCCACCAACGCATCCACACGG GGGAGAAGCCCCACGCCTGTGACATCTGTGGTCACCGCTTTCGTAACAGCTCCAACTTGGCCCGCCACCG CCGCAGCCACACTGGCGAACGGCCCTATAGCTGTCCAACCTGTGGCCGCAGTTTCCGGCGCAATGCGCAC CTGCAGCGCCACCTGATCACACACACAGGGTCAAAGCAAGAAAAGGAAGTTCCTCAGGAGTGCCCTGAGT GTGGCAAGAGCTTCAATCGCAGCTGCAACTTGCTGCGCCACCTGCTGGTTCACACCGGTGCAAGGCCTTA CTCCTGTGCACTGTGTGGCCGCAGCTTCAGCCGTAATTCACACCTGCTGCGCCACCTGCGAACCCATGCC CGGGAATCGCTGTACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289484
Insert Size	2118 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289484.1, NP_001276413.1
RefSeq Size	2504 bp
RefSeq ORF	2118 bp
Locus ID	332221
UniProt ID	Q3URR7
Gene Summary	Embryonic stem (ES) cell-specific transcription factor required to maintain ES cell pluripotency. Can both activate and /or repress expression of target genes, depending on the context. Specifically binds the 5'-[GA]CGCNNGCG[CT]-3' DNA consensus sequence. Regulates expression of POU5F1/OCT4, ZSCAN4 and ALYREF/THOC4.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) uses alternate in-frame splice sites in the 5' and 3' coding region, compared to variant 1. This results in an isoform (5) that is shorter, compared to isoform 1.

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