D7Ertd443e (NM_001199941) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4933400E14Rik; FATS; Gm497
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228689 representing NM_001199941 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTAAACTGTCTGGGGAAGGATTACTGCACACGTGCTATAGTACAAATGATCAAATCGCCCTTAAAA ACCTGCAGTCTGATGTTACAGAGAGGAAGTTGGACTTCTCCAAGGAGACACTGGCATCACAGAACACAAG AATGATATCACCTGTGGTCATTTCCCGGCTGATTGATGAAAAGAAATCTATGGAAAATGGAGCAATATTG CCACAAGCCATTGCTCAGCCTCAGCTGTGTCCCACAAAGCCAGCTTTGGCCAGGCGCGATGGAGTGAGCA TGCACAGGCGATTTGCTTTATCTCCAGACAGACTGGGAATTTTGACTCCATCAGATGATCAAGGACTGGA GACAGAACCGCTGTCTACAGGGGACAATCTAGGCAAAGGTTCCCACAGCGGTTTCTCGTCTATCACCATT ACTGCCCGACGTGTGGGCCCTCCAGCCAGCAGCTTGGTATGGGACACTTTTAGGGACCCACTGTGTCCCA AGTGCAAAGCCAAGGATGCTCTGTTCCAAGAGCCCCCAGTTCTGGCTGGTGATGCCCATCTCTGTCAGCA CAATAGACCCTTCACCTGTACAGAGTCCCCCAGCAATGGCTCCGTAGAGGGGATGAAGGTTTTCCAGGCT CACTCAAGGCTGAGTGCAAGGCAGGACTACTGGGTCACCCACACGAATGACAATGAGGACAGTTTTTCTT CAGACAATTCACCATCGAGAAAAGTCCCACTGGTGTTCAGTTCTTGTGTCCACTTCCGGGTGTCTCAGCA GTGTCCCAATGCCATTTACTATTTGGACAAGTCTCTTTCTGTTCCCCTTGAGCGACCTCAAATTGCTAGC CCCAAAATGCACAGGTCTGTCCTGTCTCTCAGCCTCCGCTGTAGTTCCCACCAGTTAACAGCAGATGGAG TAGACAGCTCAGCTAATGGAGAGCCAATCAGCACAGCTCTATCACAGGAACTCTCGGAGGGAAAGCAGGA CCTCCTAGGTCCACAATGGGGCCAGCCACAAGGTGGTCACTGGAAGGAGAGCCCAGCATTGGTGCCAGTA CATTTGGGGAGTGGCACATGCCCTCGGACTGGCTCCCCTCCACTGGAAAATGTCAAATTTGCAGACGTGG GAAGGAACCAGGTCCCAGTACGAAAGGAGAAAGAGGACCATGCAACATGTACCAGTAGCAGCCACACCAA CCAACTCTCCATCCATATTCCCGGCTGGAGTTACAGGGCTGAAACAAAAGTACTTTCTGGAAGCAAGAAG CAGCAGCAAGAAGCACAAAGAACTCTTCCTGCTTTCCCAGTGGGACAGAAGACCATTAAACACTTCCCTC CTGAAGGGGACAGTTCACCAAGCAGTGATGGTCAACCTAGCATCCTTTCAGAGTCCAATGAGAGGCAACA CCCATACTTCATGATACCCAGAGTCCCTCTGCCTGGGTTTTATTGCCCTTTACAAACTGGGTGTGCCTCT CTGCAGGAAGATGGTGCTGTTCAGATAGAAACACACTTCCCCAAAGATTACACGTGCTGTGACTTGGTTG TAAAACTAAAGGAATGTGAGAAGAACGAAGACCCCACTGTCACCCCTGAGCCCTCACCAGCAACCCCCTC TCCATCAACCCCAGAGGGAGCACAGAGCTCTGACCCATCAGAAGATAGTTACGAGCCTCTGCTGGCAAGT TCTATGACTTTGCAGGAAGCACTGGAAGTCCATAGACCTCAGTTCATTTCTCGCTCACAAGAACGGCTGC AAAAGCTTAAGCGCATGGTACAGCAAAGGAAAACCCAACAAAAGGAGAGCCTGGGGCAGAAGCAGAGCCT ACTTCCTGTCCGCGCCAACAAGAAGCAGTTCACCATTCCCCATCCTCTTAGTGATAACTTGTTCAAACCC AAAGAAAGATGCATTTCAGAGAAGGAGATGCATATGAGATCTAAAAGAATCTATAATAATTTGCCGGAAG TTAAAAAGAAAAAAGAAGAGCAGAAGAAAAGAATGATCTTACAGAGTAACCGGCTCCGAGCAGAAGTCTT CAAAAAGCAATTACTGGACCAGCTCCTTCAAAGGAATGCAGTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001199941
Insert Size	2076 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199941.1, NP_001186870.1
RefSeq Size	2559 bp
RefSeq ORF	2076 bp
Locus ID	71007
UniProt ID	D2J0Y4
Gene Summary	Tumor suppressor that is required to sustain G2/M checkpoint after DNA damage (PubMed:20843368, PubMed:20154723, PubMed:24240685). Acts as a p53/TP53 activator by inhibiting MDM2 binding to p53/TP53 and stimulating non-proteolytic polyubiquitination of p53/TP53. Exhibits ubiquitin ligase (E3) activity and assemble ubiquitin polymers through 'Lys-11'- (K11-), 'Lys-29'- (K29-) and 'Lys-63'- (K63)-linkages, independently of the ubiquitin-conjugating enzyme (E2). Promotes p53/TP53-dependent transcription of CDKN1A/p21, leading to robust checkpoint response (PubMed:24240685). Mediates CDKN1A/p21 protein stability in a ubiquitin-independent manner. Interacts with HDAC1 and prevents binding of HDAC1 to CDKN1A/p21 and facilitates the acetylation and stabilization of CDKN1A/p21 (PubMed:20154723). May have a role in the assembly of primary cilia (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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