Mapk8ip1 (NM_001202446) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	IB1; JIP-1; Jip1; mjip-2a; Prkm8ip; Skip
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228637 representing NM_001202446 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCTGGTACTGAAGATGGATTCGAGCCCAGACAATGACAGCTGGTTGGAGGATCAGTGGGAGCACT GGCTCACCCATGACATCAGCCTGGAGGAGTTTGAGGATGAAGACCTTTCGGAGATCACTGACGAGTGTGG CATCAGCCTGCAGTGCAAAGACACCCTGTCTCTCCGGATGGACCTGATCGACGCGGCAGGTGACACTCCG GGCGCCGAGGACGACGAGGAGGAGGAGGACGACGAGCTCGCTGCCCAACGACCAGGAGTGGGGCCTCCCA AAGCGGAGTCCAACCAGGATCCGGCGCCTCGCAGCCAGGGCCAGGGCCCGGGCACAGGCAGCGGAGACAC CTACCGACCCAAGAGGCCTACCACGCTCAACCTTTTCCCGCAGGTGCCGCGGTCTCAGGACACGCTGAAT AATAACTCTTTAGGCAAAAAGCACAGTTGGCAGGACCGTGTGTCTCGATCATCCTCCCCTCTGAAGACAG GAGAACAGACGCCTCCACATGAACACATCTGCCTGAGTGATGAGCTGCCACCCCAGGGCAGTCCTGTTCC CACCCAGGACCGCGGCACTTCCACCGACAGCCCTTGTCGCCGAAGTGCAGCCACCCAGATGGCACCTCCA AGCGGTCCCCCTGCCACTGCGCCTGGTGGCCGGGGCCACTCCCATCGAGACCGAATCCACTACCAGGCAG ATGTGCGGCTCGAGGCGACTGAGGAGATCTACCTGACCCCAGTGCAGAGGCCCCCAGACCCTGCAGAACC CACCTCCACCTTCATGCCACCCACGGAGAGCCGGATGTCAGTTAGCTCCGATCCAGACCCTGCCGCTTAC TCTGTAACTGCGGGGCGGCCACACCCCTCCATCAGTGAAGAGGATGAGGGCTTCGACTGCCTGTCATCCC CAGAGCGAGCTGAGCCACCAGGTGGAGGGTGGCGGGGAAGCCTCGGGGAGCCACCACCGCCTCCACGGGC CTCACTGAGCTCGGACACCAGCGCACTGTCCTACGACTCGGTCAAGTACACACTGGTGGTGGATGAACAT GCCCAGCTTGAGTTGGTGAGCCTGCGGCCGTGCTTTGGAGATTACAGTGACGAAAGCGACTCTGCCACTG TCTATGACAACTGTGCCTCTGCCTCCTCGCCCTACGAGTCAGCCATTGGTGAGGAGTATGAGGAGGCCCC TCAGCCCCGGCCTCCCACCTGCCTCTCAGAGGACTCCACCCCGGATGAGCCTGATGTCCACTTCTCTAAG AAGTTTCTGAATGTCTTCATGAGTGGCCGCTCTCGTTCCTCCAGTGCTGAGTCCTTTGGGCTGTTCTCCT GCGTCATCAATGGGGAGGAGCATGAGCAAACCCATCGGGCTATATTCAGGTTTGTGCCTCGGCATGAAGA TGAACTTGAGCTGGAAGTGGATGACCCCCTGCTGGTGGAGCTGCAGGCAGAAGACTATTGGTATGAGGCC TATAACATGCGCACCGGAGCCCGCGGGGTCTTCCCTGCCTACTATGCCATTGAGGTCACCAAGGAGCCTG AGCACATGGCAGCCCTTGCCAAAAACAGCGACTGGATTGACCAGTTCCGGGTGAAGTTCCTGGGGTCTGT CCAGGTTCCTTATCACAAGGGCAATGATGTCCTCTGTGCTGCTATGCAAAAGATCGCCACCACCCGCCGG CTCACCGTGCACTTTAACCCGCCCTCCAGCTGTGTCCTTGAGATCAGTGTCAGGGGTGTCAAGATAGGCG TCAAAGCTGATGATGCTCTGGAGGCCAAGGGAAATAAATGTAGCCACTTCTTCCAGCTAAAGAACATCTC TTTCTGTGGATACCATCCAAAGAATAACAAGTACTTTGGGTTTATCACTAAGCACCCTGCTGACCACCGG TTTGCCTGCCATGTCTTTGTGTCTGAAGATTCCACCAAAGCCCTGGCGGAGTCTGTGGGGCGTGCATTTC AGCAGTTCTACAAGCAGTTTGTGGAGTATACCTGTCCTACAGAAGATATCTACTTGGAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001202446
Insert Size	2022 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001202446.1, NP_001189375.1
RefSeq Size	3171 bp
RefSeq ORF	2022 bp
Locus ID	19099
Gene Summary	The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Required for JNK activation in response to excitotoxic stress. Cytoplasmic MAPK8IP1 causes inhibition of JNK-regulated activity by retaining JNK in the cytoplasm and thus inhibiting the JNK phosphorylation of c-Jun. May also participate in ApoER2-specific reelin signaling. Directly, or indirectly, regulates GLUT2 gene expression and beta-cell function. Appears to have a role in cell signaling in mature and developing nerve terminals. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins. Functions as an anti-apoptotic protein and whose level seems to influence the beta-cell death or survival response (By similarity). Acts as a scaffold protein that coordinates with SH3RF1 in organizing different components of the JNK pathway, including RAC1 or RAC2, MAP3K11/MLK3 or MAP3K7/TAK1, MAP2K7/MKK7, MAPK8/JNK1 and/or MAPK9/JNK2 into a functional multiprotein complex to ensure the effective activation of the JNK signaling pathway. Regulates the activation of MAPK8/JNK1 and differentiation of CD8(+) T-cells (PubMed:23963642).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence and uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (3) has a shorter and distinct N-terminus and lacks an alternate internal segment compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.