Mylk3 (NM_001297612) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	D830007F02Rik; MLCK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228605 representing NM_001297612 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAACTCTGTGCCCTGTCCCAGCACTGTGCAGGCCCAGCACCAGAGGCCAGCAGCGGGCATCAGATT ACACCCAGATATTTGCGAACAAGGAGCAAGCAGAAGTTGCTGGAGTGAAGCCAAACCATGTACTGACTAC AGGAGGTGTGCAAGCTGACGCCTCTAGGACGCTGTGGGAAGAGAGCCAAAAGGAGGACATACCTGTGCGA ACAGTGGAGGGGCTGCCTCTCATCATCAATACGTCACTGAAGGGAGCTGACCTAACCCAGGCAGGAGCCT CACTGAGGCAGGGAGTTGAAGTTCTTGGCCCAGGCCAAGTACCCCTACCCACAGAAGCAGAATCCAGGCT TCCTGAGACAGCCAGTGAGAACACTGGAGCCACCCTGGAATTGTCCGTAGCAATCGACAGAATCAGCGAG GTCCTCACTAGCCTCAAGATGTCACAAGGTGGTGGTCAAGAAACCTCATCCAGCAAGCCTGACTGTTGGC TTTCAGAAGAGGCCATGAGGCTGAGTTCAGGGCCTCTCCCTCAGCCCCTAGGACCACTAACTCCAGACAG TGACATTCACAGTGGTGATGCACTTCCCAGGATCCCTATCAATATGCAAGAGATGGCTACTCCTGGGGAG TTGCTTGAGACCCAAAGTGGCAGTCCCATTGGCTCTGCAGAAGCTCCAGGCCTTGGGACTGTGTTAGAAG ACCAGATCCCTAAAGGAGCCAGACCATTTCCACCCCTGCCAAAGAGGAGCAGCAACAATGGTGGCATGAG TGCAGAGGAGGAGATAGGGTCTGGGGCTGAGCCTATGAGAGGACCAAGCTTGGCTACAAGGGACTGGAGA GATGAGACTGTTGGGACCACAGACCTGCAGCAAGGCATAGACCCAGGAGCAGTGAGCCCTGAGCCTGGGA AGGACCACGCAGCCCAGGGCCCAGGAAGAACTGAAGCTGGAAGGCTATCTTCTGCTGCAGAGGCTGCCAT TGTGGTTCTAGATGACAGCGCAGCACCCCCAGCCCCTTTTGAACACCGGGTAGTGAGCATCAAAGATACC CTGATCTCAGCAGGCTACACGGTATCCCAACATGAAGTCTTAGGAGGGGGTCGGTTTGGCCAGGTGCACA GGTGTACAGAGAGGTCTACAGGCCTTGCACTGGCAGCCAAGATCATCAAAGTGAAGAACGTAAAGGACCG GGAGGATGTGAAGAATGAGGTCAACATCATGAACCAGCTCAGCCACGTAAACTTGATCCAACTTTATGAT GCGTTTGAGAGCAAGAGCAGCTTCACTCTGATCATGGAGTATGTGGATGGAGGCGAACTCTTTGACCGGA TCACGGATGAGAAGTACCACCTCACTGAGTTGGATGTGGTCTTGTTCACGAGGCAGATCTGTGAGGGTGT GCATTACCTGCATCAGCACTATATCCTGCACCTGGACCTCAAGCCTGAGAACATATTGTGTGTCAGCCAG ACAGGGCATCAAATTAAGATCATTGACTTTGGGCTGGCTAGAAGATACAAGCCTCGGGAGAAGCTAAAGG TGAACTTTGGTACTCCGGAGTTCCTGGCCCCAGAAGTTGTTAACTATGAGTTTGTGTCATTTCCAACAGA CATGTGGAGTGTGGGAGTTATCACCTACATGCTACTCAGTGGTTTGTCCCCATTTCTAGGGGAGACAGAT GCAGAGACCATGAATTTTATTGTGAACTGCAGCTGGGATTTCGATGCTGATACCTTCAAAGGGCTGTCGG AGGAAGCCAAGGACTTTGTTTCCCGGTTACTGGTCAAAGAGAAGAGCTGTAGGATGAGCGCCACACAGTG CCTGAAACACGAGTGGTTAAGTCACCTGCCTGCCAAAGCCTCGGGCTCCAACGTTCGCCTCAGATCCCAA CAACTGCTGCAGAAATATATGGCTCAGAGTAAATGGAAGAAACATTTCCACGTGGTGACTGCAGTCAACA GGCTACGGAAATTTCCAACGTGTCCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001297612
Insert Size	1989 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001297612.1, NP_001284541.1
RefSeq Size	2617 bp
RefSeq ORF	1989 bp
Locus ID	213435
UniProt ID	Q3UIZ8
Gene Summary	Kinase that phosphorylates MYL2 in vitro. Has been proposed to be calmodulin-dependent (PubMed:17885681), although MYL2 phosphorylation has also been observed in the presence or absence of calmodulin (PubMed:18202317). Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate first exon compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1.

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