Foxn1 (NM_001277290) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	D11Bhm185e; Fkh19; HFH-11; Hfh11; nu; nude; wh; Whn
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228572 representing NM_001277290 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTCGCTACTCCCTCCGCAGTCTGACGTCACACTTCCAGGCTCCACCCGACTGGAGGGCGAACCCC AAGGGGACCTCATGCAGGCTCCGGGCCTCCCAGACTCCCCTGCCCCACAGAACCATGCTAACTTCAGCTG CTCGTCGTTTGTGCCTGACGGCCCTCCAGAGAGGACACCCTCACTGCCCCCACACAGCCCCAGCATCGCA TCTCCAGACCCAGAGCAGATCCAGGGCCACTGCACAGCCGGACCCGGCCCGGGCTCCTTCCGCCTTTCTC CTTCAGAAAAGTATCCTGGCTTTGGCTTTGAGGAGGGCCCAGCAGGCAGCCCAGGGCGCTTCCTCAAGGG CAACCACATGCCTTTCCACCCCTACAAGAGGCACTTCCATGAGGACATCTTCTCTGAGGCCCAGACGGCC ATGGCACTTGATGGACACTCCTTTAAGACTCAGGGGGCACTGGAAGCCTTTGAGGAGATCCCTGTGGACA TGGGCGATGCTGAGGCCTTCCTGCCTAGCTTCCCAGCAGAGGCTTGGTGCAATAAACTCCCTTACCCCAG CCAGGAACACAACCAAATTCTGCAGGGGTCAGAGGTCAAGGTCAAGCCCCAAGCTCTGGACAGTGGTCCT GGGATGTACTGCTACCAGCCTCCCTTGCAACATATGTACTGTTCTTCTCAGCCTGCCTTCCATCAGTACT CCCCGGGTGGAGGCAGCTACCCTGTGCCCTACCTGGGCTCACCTCACTATCCCTATCAGAGGATTGCACC CCAGGCCAACGCCGAAGGTCACCAGCCACTCTTCCCAAAGCCCATCTACTCTTACAGCATCCTCATCTTC ATGGCCCTTAAGAACAGTAAGACCGGAAGCCTTCCAGTCAGTGAAATCTACAATTTCATGACGGAGCACT TCCCTTACTTCAAGACTGCTCCTGATGGCTGGAAGAATTCTGTTCGCCATAACCTGTCCCTCAACAAGTG CTTTGAGAAGGTGGAGAATAAATCCGGAAGTTCCTCTCGAAAGGGCTGTCTGTGGGCCCTCAATCCTTCC AAAATCGACAAGATGCAGGAAGAACTGCAGAAGTGGAAGAGGAAAGACCCCATTGCTGTGCGCAAAAGCA TGGCCAAACCAGAAGAGCTGGACAGCCTCATTGGAGACAAAAGGGAAAAACTGGGCTCTCCGCTGCTGGG CTGTCCACCCCCTGGGCTGGCAGGCCCAGGTCCCATCCGGCCCATGGCACCATCAGCTGGTCTTTCCCAG CCTCTGCACCCAATGCATCCAGCTCCAGGCCCCATGCCTGGCAAGAACCCCCTGCAGGACCTACTGGGTG GCCATGCTCCCTCCTGCTATGGGCAGACCTACCCACACCTTTCCCCCAGCCTGGCCCCTTCTGGACACCA GCAGCCATTGTTCCCACAGCCAGATGGGCATCTTGAGCTGCAGGCCCAGCCAGGCACCCCCCAGGACTCA CCTCTACCTGCCCACACACCACCCAGCCACGGTGCCAAGCTGATGGCTGAGCCTTCCTCAGCCAGGACCA TGCACGATACTCTACTGCCAGATGGAGACCTTGGGACTGACCTGGATGCTATCAACCCTTCTCTCACTGA CTTCGACTTCCAGGGAAATCTGTGGGAGCAGCTGAAGGATGACAGCTTGGCCCTGGACCCCCTCGTATTG GTGACCTCGTCGCCGACGTCATCCTCCATGTTGCCACCCCCACCAGCAGCCCATTGCTTCCCCCCAGGGC CTTGTCTGGCAGAAACAGGCAATGAGGCAGGTGAACTGGCACCTCCAGGCAGCGGCGGCTCCGGTGCTCT GGGAGACATGCACCTCAGCACTCTCTACTCCGCCTTTGTGGAACTGGAGTCCACGCCCTCCTCAGCAGCT GCCGGCCCTGCCGTGTACCTCAGTCCCGGCTCAAAGCCATTGGCTCTGGCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001277290
Insert Size	1944 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001277290.1, NP_001264219.1
RefSeq Size	3270 bp
RefSeq ORF	1944 bp
Locus ID	15218
Gene Summary	The protein encoded by this gene is part of the forkhead family or "winged-helix" transcription factors that are important in developmental processes, immune system regulation, metabolism, cancer and aging. This gene family has over 100 members, subdivided into classes (A-Q) based on phylogeny. The encoded protein is proposed to regulate development of the thymus and differentiation of keratinocytes. Mutations in this gene cause severe primary T-cell immunodeficiency and congenital alopecia. In mouse mutations of this gene underlie the phenotype of the nude mouse, which has been widely used as a model system in oncology, immunology, dermatology, and transplantation studies. In humans mutations in this gene have been correlated with T-cell immunodeficiency, the skin disorder congenital alopecia, and nail dystrophy. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Apr 2013] Transcript Variant: This variant (2) uses an alternate in-frame acceptor splice site in the 5' coding region compared to variant 1. It encodes isoform 2 which is shorter compared to isoform 1.

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