Rhbdf1 (NM_001291818) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	C16ORF8; Dist; Dist1; Egfr-rs; mKIAA4242
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228562 representing NM_001291818 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTTCCGGGCCGCTGCAGCATTGGTGAAGGGTCGGTCTATTAGGGATGGCACTTTACGAAGGGGAC AGCGCCGAAGCTTTACCCCTGCCAGCTTTCTTGAGGAAGACATGGTGGACTTCCCTGATGAGTTGGATAC ATCTTTCTTTGCTCGGGAAGGTGTCCTCCACGAGGAGATGTCCACGTACCCAGATGAGGTGTTTGAGTCC CCATCAGAGGCAGCGCTCAAGGACTGGGAGAAAGCCCCGGATCAGGCCGACCTCACGGGTGGGGCCCTGG ACCGCAGTGAGCTTGAGAGAAGCCACTTGATGTTGCCCCTGGAACGAGGCTGGAGAAAGCAGAAGGAAGG CGGCCCTCTAGCCCCGCAGCCCAAGGTGCGGCTTCGCCAGGAGGTGGTCAGTGCGGCGGGACCCAGGAGG GGTCAGCGTATCGCTGTGCCAGTGCGCAAACTCTTCGCTCGGGAAAAGCGGCCTTATGGTCTGGGGATGG TGGGACGGCTCACCAACCGCACGTACCGGAAACGCATCGACAGCTATGTCAAGCGGCAGATCGAGGACAT GGATGATCACAGGCCCTTCTTCACTTACTGGCTCACCTTTGTGCACTCACTGGTCACCATTCTGGCTGTG TGCATCTATGGCATAGCGCCCGTGGGCTTCTCGCAGCATGAGACGGTGGACTCGGTACTTCGGAAGCGGG GTGTCTATGAGAATGTCAAATACGTCCAGCAGGAGAACTTCTGGATTGGCCCCAGCTCGGAGGCCCTCAT TCACTTGGGTGCCAAGTTCTCACCCTGCATGCGTCAGGACCCACAGGTGCACAGCTTCATCCTGGCTGCC CGGGAGCGTGAAAAGCACTCGGCTTGCTGTGTACGCAATGATCGGTCTGGGTGTGTGCAGACTTCCAAGG AGGAGTGCTCGTCCACGTTGGCGGTGTGGGTGAAGTGGCCCGTTCATCCTAGTGCCCCAGACCTTGCAGG CAACAAGAGACAGTTTGGTTCTGTCTGCCACCAGGACCCCAGGGTATGTGACGAGCCTTCCTCTGAGGAT CCCCACGAGTGGCCAGAAGATATCACCAAGTGGCCGATCTGCACCAAAAGCAGCGCGGGGAACCACACTA ACCACCCTCACATGGATTGTGTCATAACAGGCCGGCCTTGCTGCATCGGCACCAAGGGCAGGTGTGAGAT CACCTCCCGGGAGTACTGTGACTTCATGAGGGGCTACTTCCATGAGGAGGCCACACTCTGCTCTCAGGTG CACTGCATGGACGATGTCTGTGGCCTCCTGCCTTTCCTCAATCCTGAGGTGCCTGACCAGTTCTACCGCC TGTGGCTGTCCCTCTTCCTGCACGCTGGGATCCTGCACTGCTTGGTGTCCGTCTGCTTCCAGATGACGGT CCTGCGGGACCTGGAGAAGCTGGCAGGCTGGCACCGCATAGCCATCATCTACCTGCTGAGTGGTATCACG GGCAATCTAGCCAGTGCCATCTTCCTGCCATACCGGGCAGAGGTAGGTCCGGCCGGCTCCCAGTTTGGCA TCCTGGCCTGCCTCTTTGTGGAGTTGTTCCAGAGCTGGCAGATCCTGGCCCGACCCTGGCGTGCCTTCTT CAAGCTCCTGGCCGTGGTGCTCTTCCTCTTTGCCTTTGGGCTGCTGCCCTGGATCGACAACTTCGCCCAC ATCTCGGGCTTTGTCAGCGGCCTCTTCCTTTCCTTTGCCTTCCTGCCTTACATCAGCTTTGGCAAGTTCG ACCTGTACCGCAAGCGCTGCCAGATCATCATCTTCCAGGTCGTCTTCCTGGGCCTGCTGGCCGGCCTGGT GGTCCTCTTCTACTTCTACCCGGTGCGCTGTGAATGGTGCGAGTTCCTCACCTGCATCCCTTTCACGGAC AAGTTCTGTGAAAAGTACGAGCTGGACGCTCAGCTCCACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291818
Insert Size	1932 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291818.1, NP_001278747.1
RefSeq Size	2836 bp
RefSeq ORF	1932 bp
Locus ID	13650
Gene Summary	Regulates ADAM17 protease, a sheddase of the epidermal growth factor (EGF) receptor ligands and TNF, thereby plays a role in sleep, cell survival, proliferation, migration and inflammation. Does not exhibit any protease activity on its own.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) use an alternate splice junction at the 5' end of an exon and initiates translation at a downstream start codon compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1.

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