Cacnb1 (NM_001282977) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	CAB1; Cchb1; Cchlb1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228558 representing NM_001282977 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCAGAAGAGCGGCATGTCCCGGGGCCCTTACCCACCTTCCCAAGAGATCCCTATGGAGGTCTTCG ACCCCAGCCCACAGGGCAAGTACAGCAAGAGGAAAGGGCGGTTCAAAAGGTCAGACGGGAGTACGTCCTC GGATACAACATCCAACAGCTTCGTCCGCCAGGGCTCAGCAGAGTCCTACACGAGCCGACCATCAGACTCT GATGTGTCTCTGGAGGAGGACCGGGAAGCCTTAAGGAAGGAGGCAGAGCGCCAGGCCTTAGCCCAGCTCG AGAAAGCCAAGACCAAACCAGTGGCTTTTGCTGTTCGGACAAATGTTGGCTACAATCCGTCTCCAGGGGA TGAGGTGCCTGTACAGGGAGTGGCCATCACCTTTGAGCCCAAGGACTTCCTACACATCAAGGAGAAGTAC AATAATGACTGGTGGATTGGGCGGCTGGTGAAGGAAGGCTGCGAGGTTGGCTTCATCCCCAGCCCGGTCA AACTGGACAGCCTTCGTCTGCTGCAGGAACAGACCCTGCGCCAGAACCGCCTCAGCTCCAGCAAGTCAGG TGACAACTCCAGTTCCAGTCTGGGAGATGTGGTGACTGGCACCCGCCGCCCCACACCCCCTGCCAGTGGT AATGAAATGACTAACTTTGCCTTTGAGCTAGACCCCCTAGAGTTAGAGGAGGAGGAGGCAGAGCTAGGGG AGCACGGCGGCTCAGCCAAGACTAGCGTGAGCAGTGTCACCACGCCGCCACCCCACGGCAAGCGCATCCC CTTCTTTAAGAAGACAGAGCACGTGCCCCCCTATGACGTGGTGCCTTCCATGAGGCCCATCATCCTGGTG GGACCGTCGCTCAAGGGCTATGAGGTGACAGACATGATGCAGAAAGCGTTGTTTGACTTCCTCAAGCATC GGTTTGATGGCAGGATTTCCATCACCCGGGTAACAGCTGACATTTCCCTGGCCAAACGCTCCGTCCTCAA CAACCCCAGCAAACACATCATCATTGAGCGCTCCAACACGCGTTCCAGCCTGGCTGAGGTACAGAGTGAA ATTGAGAGGATCTTCGAGCTGGCCCGGACCTTGCAGCTGGTCGCCTTGGACGCTGACACCATCAACCACC CAGCCCAGCTCTCTAAAACGTCGCTGGCCCCCATCATTGTTTACATCAAGATCACATCTCCCAAGGTACT GCAGAGGCTCATCAAATCCCGAGGGAAGTCTCAATCCAAACACCTCAATGTCCAAATAGCAGCCTCGGAG AAGCTGGCACAGTGTCCCCCCGAAATGTTTGACATAATCCTGGACGAGAACCAATTGGAAGATGCCTGCG AGCACCTGGCTGAGTACTTGGAAGCCTACTGGAAGGCCACACATCCGCCTAGCAGCACGCCACCCAATCC GCTGCTGAACCGCACCATGGCTACCGCAGCTCTGGCTGCCAGCCCTGCCCCCGTCTCCAACCTCCAGGGA CCCTACCTTGCTTCCGGGGACCAGCCGCTGGACCGGGCCACTGGGGAACATGCCAGTGTGCACGAGTACC CCGGGGAACTGGGCCAGCCCCCAGGCCTTTACCCCAGCAACCACCCACCTGGCCGGGCAGGCACCCTGCG GGCGCTATCCCGCCAAGACACCTTTGATGCTGACACCCCCGGCAGCCGAAATTCTGCCTACACGGAGCCG GGAGACTCGTGTGTGGACATGGAGACAGACCCCTCAGAGGGCCCAGGGCCTGGAGACCCTGCAGGGGGAG GCACACCACCAGCCCGGCAGGGCTCCTGGGAAGACGAGGAAGACTATGAGGAGGAGATGACCGACAACAG GAACCGGGGCCGGAATAAGGCCCGCTACTGTGCGGAGGGTGGTGGGCCGGTTCTGGGGCGCAATAAGAAT GAGCTGGAGGGCTGGGGACAAGGCGTCTACATCCGCTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001282977
Insert Size	1929 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282977.1, NP_001269906.1
RefSeq Size	3524 bp
RefSeq ORF	1929 bp
Locus ID	12295
Gene Summary	Regulatory subunit of L-type calcium channels. Regulates the activity of L-type calcium channels that contain CACNA1A as pore-forming subunit (By similarity). Regulates the activity of L-type calcium channels that contain CACNA1C as pore-forming subunit and increases the presence of the channel complex at the cell membrane. Required for functional expression L-type calcium channels that contain CACNA1D as pore-forming subunit. Regulates the activity of L-type calcium channels that contain CACNA1B as pore-forming subunit (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) represents the longest transcript and encodes the longest isoform (E). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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