Tktl2 (NM_001271574) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4933401I19Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228513 representing NM_001271574 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTTGGCCAGAGATGCCAAGCTGGAGTCGGACACCTTTCAGGTGCTTCAGGACGTGGCCAATCGCC TGCGAATCCACTCAATCAGGGCCACGTGTGCCTGCAGCTCCGGACATCCCACGTCGTGCTGTAGTGTGGC AGAGATCATGGCTGTGCTCTTCTTCCATACCATGAGGTACAAACAGGCAGACCCAGAACACCCGGACAAT GACCGCTTTGTCCTCTCCAAGGGCCACGCCGCGCCCATCCTCTATGCGGTTTGGGTGGAGGTGGGCCGTA TCTGTGAATCGGACTTGCTGAACTTGCGAAAAATTCACTGCGACCTGGAGGGTCATCCCACCCCACGACT GTCGTTTGTTGATGTGGCAACAGGGTCCCTTGGGCAAGGACTGGGTGCTGCCTGTGGAATGGCTTACACC GGCAAATACTTTGACAAGGCCAGCTACCGCGTGTTCTGCCTCATGGGGGATGGTGAGTCCTCGGAAGGTT CTGTCTGGGAGGCCTTGGCCTTTGCTTCCCACTACAACTTGGACAATCTCGTGGCAATCTTTGACGTGAA CCGCTTGGGACAAAGCGGCACAGCACCCCTAGAGCACTGTACAGCCGTTTATGAGAAGCGCTGCCAAGCG TTTGGGTGGAATACTTACGTGGTGGATGGTCATGATGTTGAAGCTCTCTGCCAGGCCTTTTGGAAAGCAG CTCAAGTCAAGAATAAGCCTACTGCCCTGATTGCCAAGACCTTCAAGGGTAGAGGTATTCCAAATGTCGA GGATGCGGAAAATTGGCATGGAAAGCCTATGCCAAAAGACAGAGCTGATGGGATTGTCAAATTAATTGAG AATCGGATACAGACAAACCGAAATCTCACACCGAAACCCCCCATTGAAGACTCGCCCAGGATCAGCATGT CCAATACAAAAATGACCTCTCTGCCTGTCTACAAACTGGGAGACATGATAGCTACTCGTGAAGCATACGG CTTGGCTCTGGCTAAATTGGGCCAATCTAACCAAAGAGTTATCGTTCTAGATGGCGACACAAAAAACTCT ACCTTTTCAGAAGTATTCAAGAAAGAGCACCCTGAGCGTTTCATAGAGTGCTTTATCGCTGAGCAAAACA TGGTAAGTGTGGCCCTGGGGTGTGCCACACGAGGACGAACTATTGCTTTCGTTAGTACCTTTGCTGCCTT CCTGACCCGAGCCTTTGATCAGATCCGAATGGGAGCCATCTCTCAAACCAACATCAACTTCGTTGGTTCC CACTGCGGGGTGTCTGTTGGAGAAGACGGACCTTCCCAGATGGCCCTGGAGGACCTAGCCATGTTCAGAA GCATCCCCAACTGCACTGTTTTCTATCCAAGTGATGCTGTCTCGACAGAACACGCTGTTTATCTGGCAGC CAACACGAAGGGAATGTGCTTCATTCGTACCACTCGGCCAAAAACAGCAGTTATTTATACAGCTGAAGAA AACTTTGTGATTGGACAGGCCAAGGTGATCCGCCAGAGTGCAGTCGACAAGGTGACAGTTATTGGAGCAG GGGTTACTCTGCATGAAGCCTTAGTAGCTGCTGAGGAGCTTTCTCAACAAGGGATTTTTATTCGTGTCAT TGACCTGTTCACTATCAAACCTCTGGATGCTGTCACCATTATCCAAAGTGCCAAAGCCACAGGTGGCCAG ATCATCACTGTGGAAGATCACTACCGAGAAGGTGGCATTGGTGAAGCTGTGTGTGCAGCCATCTCTAGAG AGCCTGACATAGTCGTTCGTCAACTTGCAGTAACAGAAGTACCTCGAAGCGGGAAACCTAGCGAGCTACT GGATATGTTTGGAATCAGTGCCAGACACATTATAGCAGCTGTGAAAGATACCGTAATGAAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271574
Insert Size	1884 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271574.1, NP_001258503.1
RefSeq Size	6368 bp
RefSeq ORF	1884 bp
Locus ID	74419
UniProt ID	Q9D4D4
Gene Summary	Plays an essential role in total transketolase activity and cell proliferation in cancer cells; after transfection with anti-TKTL1 siRNA, total transketolase activity dramatically decreases and proliferation was significantly inhibited in cancer cells. Plays a pivotal role in carcinogenesis (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) contains an alternate exon structure in the 3' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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