Papss2 (NM_001201470) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1810018P12Rik; AI159688; Atpsk2; AtpsU2; bm; Sk2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228476 representing NM_001201470 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGGGGTCAAGAAGCAGAAGACGGACCAGCAAAAATCCACCAATGTGGTCTACCAGGCCCATCATG TGAGCAGGAACAAGAGAGGACAAGTGGTTGGAACCAGGGGAGGATTCCGAGGATGTACCGTGTGGCTAAC AGGTCTCTCTGGTGCTGGGAAAACAACCATAAGCTTTGCTTTGGAAGAGTACCTTGTATCTCACGCCATC CCATGTTACTCTCTGGATGGGGACAATGTCCGTCATGGCCTTAATAAGAACCTGGGATTCTCTGCGGGGG ACCGAGAAGAGAATATCCGCCGGATCGCGGAGGTGGCCAGGCTCTTTGCCGACGCCGGCCTGGTTTGCAT CACCAGCTTTATCTCTCCTTTTGCAAAGGATCGTGAGAATGCCCGAAAAATCCACGAATCAGCAGGACTC CCGTTCTTTGAGATCTTTGTAGATGCGCCTTTAAATATCTGTGAAAGCCGAGACGTAAAAGGACTCTACA AACGAGCCCGAGCAGGAGAGATTAAAGGGTTTACAGGCATCGATTCTGACTATGAGAAACCTGAAACTCC AGAGTGTGTGCTGAAGACCAACCTGTCTTCAGTAAGCGACTGTGTGCAACAGGTGGTGGAACTTTTGCAG GAGCAGAACATTGTACCCCACACCACCATCAAAGGCATCCACGAACTCTTTGTGCCAGAAAACAAAGTCG ATCAAATCCGAGCTGAGGCAGAGACTCTCCCATCACTACCAATTACCAAGCTGGATCTGCAGTGGGTGCA GATTCTGAGTGAAGGCTGGGCCACTCCCCTCAAAGGCTTTATGCGGGAGAAGGAGTACTTGCAAACTCTA CACTTCGACACTCTACTGGACGATGGAGTCATCAACATGAGTATTCCCATTGTATTGCCCGTTTCTGCGG ATGACAAGGCACGGCTCGAAGGGTGCAGCAAATTTGCCTTGATGTACGAAGGTCGGAGGGTCGCTCTATT ACAGGACCCTGAATTCTATGAGCATAGGAAAGAGGAGCGCTGTTCTCGTGTGTGGGGAACAGCCACTGCA AAGCACCCCCATATCAAAATGGTGATGGAAAGTGGGGACTGGCTTGTTGGTGGAGACCTACAGGTGCTAG AGAGAATAAGGTGGGACGATGGGCTGGACCAATACCGCCTTACGCCTCTGGAGCTCAAACAGAAGTGTAA AGACATGAATGCTGATGCCGTGTTTGCATTCCAGTTGCGCAATCCTGTCCACAATGGTCATGCCCTCCTG ATGCAGGACACCCGCCGCAGGCTCCTGGAGAGGGGTTACAAGCACCCAGTCCTCCTGCTCCACCCTCTTG GGGGCTGGACCAAGGACGATGACGTACCTCTGGAATGGAGGATGAAACAGCATGCAGCTGTACTGGAGGA AAGGGTCCTGGATCCCAAGTCAACTATTGTTGCCATCTTTCCATCTCCTATGTTATATGCTGGTCCCACA GAGGTCCAGTGGCATTGCAGATGCCGGATGATTGCAGGAGCCAATTTCTACATTGTGGGTAGGGATCCCG CAGGAATGCCCCATCCTGAGACAAAGAAAGACCTATATGAACCCACCCACGGGGGCAAGGTCTTGAGTAT GGCCCCTGGCCTTACCTCTGTGGAAATAATTCCGTTCCGAGTGGCTGCCTACAATAAAATTAAAAAGGCC ATGGACTTTTATGATCCAGCAAGGCACGAGGAGTTTGACTTCATCTCAGGAACTCGCATGAGGAAGCTCG CCCGGGAAGGAGAAGATCCCCCAGATGGCTTCATGGCCCCGAAAGCGTGGAAAGTGTTGACAGATTACTA CAGGTCTCTGGAGAAGACCAACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001201470
Insert Size	1845 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001201470.1, NP_001188399.1
RefSeq Size	3599 bp
RefSeq ORF	1845 bp
Locus ID	23972
Gene Summary	Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. May have an important role in skeletogenesis during postnatal growth.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.