Zmynd11 (NM_001199141) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2210402G22Rik; BS69
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228436 representing NM_001199141 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACGTTTAACAAAAAGACGACAGGCGGATACAAAAGCTATCCAGCATCTTTGGGCAGCCATTGAAA TTATCAGAAATCAGAAGCAGATTGCCAACATTGATCGTATTACAAAGTATATGTCCCGAGTCCACGGTAT GCATCCCAAAGAGACCACCCGCCAGCTGAGCTTAGCTGTGAAAGATGGTCTTATTGTCGAAACTCTAACA GTGGGCTGCAAAGGTTCAAAAGCTGGTATTGAACAGGAAGGATATTGGTTACCAGGAGATGAGATTGACT GGGAAACAGAAACTCATGACTGGTATTGTTTTGAATGCCATTTGCCTGGAGAGGTGTTGATATGTGACCT GTGTTTTCGTGTGTATCATTCCAAGTGTTTGTCTGATGAGTTCAGGCTTAGAGACAGCAGTAGTCACTGG CAGTGCCCAGTTTGCAGGAGCATTAAGAAGAAGCACTCTAACAAGCAGGAAATGGGCACCTACTTGAGGT TCATCGTCTCCCGAATGAAGGAGCGGGCTATAGACCTTAATAAGAAAGGAAAGGACAGTAAGCATCCTAT GTACCGGAGGCTGGTCCACTCAGCTGTTGACGTCCCTACCATACAAGAGAAGGTGAATGAGGGGAAGTAC CGGAGTTACGAGGAGTTCAAGGCTGACGCACAGCTGCTTCTCCACAACACAGTGATCTTCTATGGAGCAG ACAGTGAGCAGGCGGACATTGCACGGATGTTATATAAAGACACGTGTCACGAGCTGGACGAGCTGCAGCT TTGCAAGAACTGCTTCTATCTGTCAAACGCACGGCCCGACAACTGGTTCTGCTACCCTTGCATACCTAAT CATGAGCTGGTTTGGGCTAAAATGAAAGGTTTTGGGTTTTGGCCAGCCAAAGTCATGCAGAAAGAAGACA ATCAAGTGGATGTTCGCTTCTTTGGCCATCACCACCAGAGAGCCTGGATTCCTTCTGAAAACATCCAGGA TATCACGGTCAATGTTCACCGACTGCACGTGAAGCGCAGCATGGGCTGGAAGAAGGCCTGTGACGAGCTG GAACTGCATCAGCGCTTCCTCCGTGAAGGAAGGTTCTGGAAGTCTAAGAATGAGGACCGAGGTGAGGAAG AGGCAGAATCCAGTATCTCCTCCACCAGTAACGAGCAGCTGAAGGTCACTCAAGAGCCAAGAGCAAAGAA AGGACGACGTAATCAGAGTGTGGAGCCCAAAAAGGAAGAACCCGAGCCAGAAACGGAAGCAGTGAGTTCC AGCCAGGAAATCCCCACAATGCCTCAGCCAATTGAGAGGGTGTCAGTGTCAACCCAGACCAAGAAGTTAA GTGCCTCTTCCCCACGAATGCTGCATCGAAGCACTCAGACGACGAGCGACGGCGTCTGTCAGAGCATGTG CCACGACAAGTACACCAAAATTTTTAATGACTTTAAAGACAGGATGAAGTCGGACCACAAGCGGGAAACA GAGAGAGTGGTCCGTGAAGCGCTGGAAAAGTTGCGTTCTGAAATGGAAGAAGAAAAAAGACAGGCCGTGA ACAAAGCTGTGGCCAGCCTGCAGGGCGACATGGACAGAAAGGGTAAGCAGCTGAAGGAGAAGTGCAAGGA AGAGTTTGTAGAGGAGATCAAGAAGCTAGCGGCGCAGCACAAGCAGCTCATTTCCCAGACCAAGAAGAAG CAGTGGTGCTACAACTGTGAGGAGGAGGCCATGTACCACTGCTGCTGGAACACGTCCTACTGCTCCATCA AGTGCCAGCAGGAGCACTGGCACGCAGAGCACAAGCGCACCTGCCGCCGGAAGAGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001199141
Insert Size	1809 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199141.1, NP_001186070.1
RefSeq Size	4132 bp
RefSeq ORF	1809 bp
Locus ID	66505
UniProt ID	Q8R5C8
Gene Summary	Chromatin reader that specifically recognizes and binds histone H3.3 trimethylated at 'Lys-36' (H3.3K36me3) and regulates RNA polymerase II elongation. Does not bind other histone H3 subtypes (H3.1 or H3.2). Colocalizes with highly expressed genes and functions as a transcription corepressor by modulating RNA polymerase II at the elongation stage (PubMed:24590075). Binds non-specifically to dsDNA (By similarity). Acts as a tumor-suppressor by repressing a transcriptional program essential for tumor cell growth (PubMed:24590075).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR and uses an alternate in-frame splice site in the 3' coding region, compared to variant 8. The encoded isoform (gamma) is shorter than isoform beta. Variants 3, 4 and 5 all encode isoform gamma.

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