Cep63 (NM_001301689) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4921501M07; AL450317.13gm1; AW107703; CD20; CD20R; D9Mgc41; D9Mgc48e; ET2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228412 representing NM_001301689 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGGTTGCGGCTGCCCGGTCGTCGCGCCCCTGGGTTCCTCCTCCTCCCTATGGGACTCTGGGCTGG CCGGCCAGACTACAGCAATGAATTGGTGGCCTAGCAACCTCCAAAGATGGAAAAGCCGAAGTTCGAGGAC ACAGAGCTGTTGCCAAAACAAAGAGGATTTAGAGATGGAGGCTTTGTTGGAAGGAATACAAAATCGGGGG CATAGTGGGGGATTTTTGACATCCTGTGAAGCAGAACTGCAAGAGCTCATGAAACAGATCGATATAATGG TGGCTCATAAGAAGTCGGAGTGGGAGGGGCAGACGCACGCTCTGGAGACTTGCTTGGACATCCGTGATCG GGAGCTGAAGGCTCTGAGGAGTCAGCTGGACATGAAGCACAAAGAGGTTGGAATATTGCATCAGCAGATA GAAGAACATGAGAAAACCAAGCAAGAAATGGCCATGGAGTACAAGGAGGAGTTACTGAAGCTGCAGGAGG AATTAAGCAGACTGAAGAGAAGCTATGAAAAGCTGCAGAAAAAGCAACTAAGAGAATTTAGAGGAAATAC CAAAAGTTTTCGAGAGGATCGGTCTGAGATTGAGAGGCTAACTGGAAAAATAGAGGAATTCCGACAGAAG TCTCTGGACTGGGAGAAGCAGCGTCTGATTTATCAGCAACAGGTATCTTCTCTGGAAGCACAGAGGAAGG CTCTGGCTGAACAGTCGGAGATAATCCAGGCTCAGCTTGCCAACCGGAAACAGAAATTAGAGTCCGTGGA ACTATCTAGCCAGTCAGAAATTCAACACCTGAACAGTAAGCTTGAGCGGGCTAAGGACACCATCTGTGCC AATGAGCTGGAAATAGAGCGCCTTAACATAAGGGTCAACGACCTGATGGGAACCAATATGACTATCCTGC AGGACCATCGGCAGAAGGAGGAGAAACTAAGGGAATCTGAGAAGTTACTAGAGGCTCTGCAGGAAGAACA GAAAGAGTTGAAGGCAAGTCTTCAATCTCAAGAAACCTTCATCCTTGAGGCAAAAATGCAGGAGAAACTG CAAACGACATTAAAGGCAGTTGGCACTCAGCAGTCCGTAGAAAGGCCTCTGGAGGACTGTCAGAAGGAAA GGAAGTACAGCTCTCCAGGGCAAGGAGTCTTGGATAATGTGCTCTCCCAGCTGGACTTCAGCCACAGCAG TGAAGAACTCCTGCAGGCAGAGGTGACGCGTCTTGAAGGCAGTTTAGAATCTGTGAGTGCAACATGCAAA CAGCTGAGCCAAGAACTAATGGAAAAATACGAAGAGCTGAAGAGGATGGAAGGGCATAACAATGAGTACC GGACAGAGATAAAGAAGTTGAAGGAACAGATTCTGCAGGCTGATCAGACCTACAGTTCTGCCCTGGAAGG AATGAAGATGGAAATCTCCCAGCTAACTCGGGAGTTGCACCAGCGAGATATCACTATTGCTTCTGCCAAG TGTTCCTCCTCAGACATGGAAAAGCAGCTGAAGGCAGAGATGCAGAAGGCAGAAGAAAAGGCTGTAGAGC ATAAGGAGATTCTAAGTCAGCTGGAATCTCTCAAATTAGAAAATCATCGTCTTTCTGAAACAGTGATGAA GCTGGAACTGGGCTTGCATGAGGGCTCGCTGCCCACCTCTCCTCTGGGATCGATAGCTACCAGGTTTCTG GAAGAGGAGGAGCTGAGGTCTCACCATATTCTAGAGCGCCTGGACGCCCACATTGAAGAACTGAAAAGAG AGAGTGAGAAGACGGTGAGGCAGTTCACAGCCCTCGTGTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001301689
Insert Size	1791 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301689.1, NP_001288618.1
RefSeq Size	2210 bp
RefSeq ORF	1791 bp
Locus ID	28135
Gene Summary	This gene encodes a subunit of the centrosome, the main microtubule-organizing center of the cell. The encoded protein associates with another centrosomal protein, CEP152, to regulate mother-centriole-dependent centriole duplication in dividing cells. Disruption of a similar gene in human has been associated with primary microcephaly (MCPH). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014] Transcript Variant: This variant (2) lacks multiple alternate in-frame exons in the 3' coding region compared to variant 1. It encodes isoform 2 which is shorter than isoform 1.

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