Adam17 (NM_001291871) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	CD156b; Tac; Tace
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228390 representing NM_001291871 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCGTGGAGAAGAGAGCACCACTACAAATTACTTAATAGAGCTAATTGACCGAGTTGATGACATAT ACCGGAACACGTCGTGGGATAATGCAGGGTTTAAAGGGTATGGAGTGCAGATAGAGCAGATTCGAATTCT CAAGTCTCCACAAGAGGTAAAACCTGGTGAAAGACACTTCAATATGGCAAAAAGTTTCCCAAACGAAGAG AAGGATGCTTGGGATGTGAAGATGCTATTAGAGCAATTTAGCTTTGATATAGCTGAAGAAGCATCTAAAG TCTGCCTGGCTCATCTTTTCACGTACCAGGATTTTGATATGGGAACTCTTGGATTAGCTTACGTTGGTTC TCCCAGAGCAAACAGTCATGGAGGGGTTTGTCCAAAAGCTTATTACAACCCAACTGTGAAGAAAAACATC TATTTAAATAGTGGTCTGACTAGTACTAAAAATTATGGCAAAACTATTCTCACAAAGGAAGCTGACCTGG TTACAACTCATGAATTGGGACATAATTTTGGAGCAGAACATGACCCTGATGGGCTAGCAGAATGTGCCCC AAATGAGGACCAAGGAGGAAAGTATGTCATGTATCCCATAGCTGTGAGCGGTGACCACGAGAATAATAAG ATGTTTTCAAACTGCAGTAAACAGTCCATCTACAAGACCATAGAAAGTAAGGCTCAAGAGTGCTTCCAGG AGCGCAGCAACAAGGTGTGTGGCAACTCCAGGGTGGATGAAGGAGAGGAGTGTGACCCGGGTATTATGTA CCTGAACAACGACACCTGCTGCAATAGTGACTGCACACTGAAGCCGGGTGTGCAGTGCAGTGATAGGAAC AGTCCTTGCTGTAAAAACTGTCAGTTTGAGACGGCGCAGAAGAAGTGCCAGGAGGCTATTAATGCTACAT GCAAAGGAGTGTCTTACTGCACAGGGAATAGCAGTGAGTGCCCCCCACCCGGAGATGCTGAAGATGACAC TGTGTGCTTGGACCTTGGCAAGTGCAAGGCTGGGAAATGCATCCCTTTCTGCAAGAGGGAGCAGGAGCTG GAGTCCTGCGCATGCGTTGACACTGACAACTCGTGCAAGGTGTGCTGCAGGAACCTTTCTGGCCCGTGTG TGCCGTACGTCGATGCAGAGCAAAAGAACTTGTTTTTGAGGAAAGGGAAGCCATGTACAGTAGGGTTTTG CGACATGAATGGCAAATGTGAGAAACGAGTACAGGACGTAATTGAGCGATTTTGGGATTTCATTGACCAG CTGAGCATCAACACTTTTGGGAAGTTTCTGGCAGATAACATCGTTGGGTCTGTTCTGGTTTTCTCCTTGA TATTTTGGATTCCTTTCAGCATTCTTGTCCACTGTGTGGATAAGAAACTGGACAAGCAGTATGAATCCCT GTCTCTGTTTCATCACAGTAACATTGAGATGCTGAGCAGCATGGACTCAGCATCTGTTCGCATCATCAAG CCCTTTCCTGCACCCCAGACTCCAGGTCGTCTGCAGGCCCTGCAGCCAGCTGCCATGATGCCGCCAGTAC CTGCAGCTCCAAAACTGGACCACCAGAGGATGGACACCATCCAGGAAGACCCCAGCACAGACTCACATGC AGATGATGACGGTTTTGAGAAGGACCCCTTCCCCAACAGCAGCACAGCTGCCAAGTCCTTTGAGGATCTC ACAGACCACCCAGTCACCAGGAGCGAAAAGGCGGCCTCATTCAAGCTGCAGCGTCAGAGCCGAGTTGACA GCAAAGAGACAGAGTGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291871
Insert Size	1770 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291871.1, NP_001278800.1
RefSeq Size	4470 bp
RefSeq ORF	1770 bp
Locus ID	11491
UniProt ID	Q9Z0F8
Gene Summary	This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. The encoded preproprotein undergoes proteolytic processing to generate a mature enzyme that is involved in the proteolytic release of membrane-bound proteins in a process called ectodomain shedding. Mice lacking the encoded protein die in utero or fail to survive beyond one week of age. Alternative splicing results in multiple transcript variants encoding different isoforms, some of which may undergo similar processing. [provided by RefSeq, May 2016] Transcript Variant: This variant (4) uses an alternate 5' splice site, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded protein (isoform 4) has a shorter N-terminus than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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