Slu7 (NM_148673) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AU018913; D3Bwg0878e; D11Ertd730; D11Ertd730e
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228384 representing NM_148673 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCGGCCGCTGTGGACCCAGTTAGTGCGACACCCATGACGGGATCAAAGGAGATGAGTTTGGAGG AGCCGAAAAAGATGACCCGAGAGGACTGGAGGAAGAAGAAGGAGCTAGAAGAACAGAGGAAACTGGGCAA TGCTCCTGCAGAAGTCGATGAAGAGGGAAAAGATATCAACCCTCATATTCCTCAGTATATTTCTTCGGTT CCATGGTACATTGATCCATCAAAGAGACCCACTTTAAAGCATCAGAGACCACAGCCAGAGAAACAGAAGC AGTTCAGTTCATCTGGGGAGTGGTACAAGCGAGGCGTAAAGGAGAATTCTATAACTACCAAGTACCGCAA AGGTGCATGTGAGAATTGTGGAGCCATGACACACAAGAGGAAAGATTGCTTTGAGAGACCGAGGCGGGTT GGAGCTAAATTCACAGGAACTAACATTGCTCCAGACGAGCACGTCCAGCCCCAGCTGATGTTTGACTATG ATGGGAAGAGAGACCGCTGGAATGGCTACAATCCGGAAGAGCACATGAAGATTGTCGAGGAATACGCCAA GGTTGATCTGGCAAAACGGACATTGAAAGCACAGAAACTGCAAGAAGAGTTAGCCTCTGGAAAATTAGTG GAGCAAGCTAACTCTCCGAAACACCAGTGGGGAGAAGAGGAACCCAACTCTCAGATGGAAAAGGATCATA ACAGTGAGGATGAAGACGAAGACAAATATGCGGATGACATTGACATGCCTGGGCAGAATTTCGACTCTAA GAGACGCATTACTGTTCGCAATCTCCGGATTCGTGAAGATATTGCAAAATATTTGAGAAATTTAGATCCA AATTCTGCCTATTATGATCCAAAAACCAGAGCGATGAGAGAGAATCCTTACGCCAATGCAGGGAAGAATC CAGATGAAGTGAGCTACGCTGGAGATAACTTTGTTCGATACACAGGAGATACCATCTCCATGGCTCAAAC ACAACTGTTTGCTTGGGAAGCCTACGACAAGGGGTCTGAAGTGCATCTCCAGGCAGATCCGACAAAACTA GAGCTGCTGTATAAGTCCTTCAAAGTCAAAAAAGAAGACTTCAAAGAGCAGCAGAAGGAAAGCATCCTGG AAAAGTACGGTGGCCAAGAACACCTGGACGCCCCTCCAGCTGAGCTGCTCTTAGCCCAGACAGAAGACTA CGTGGAGTACTCCAGGCATGGCACAGTCATTAAAGGCCAGGAGCGGGCTGTCGCCTGCTCCAAGTACGAG GAAGACGTGAAGATCAATAACCACACGCATATCTGGGGATCTTACTGGAAAGAAGGCCGCTGGGGATACA AATGTTGTCACTCGTTTTTTAAGTATTCCTACTGTACTGGAGAAGCTGGGAAGGAGAGCGTTAACTCAGA GGAGTGTATTATAACTGGTGCGACTGCAGAAGAGTCTGTGAAGAAACCTCAGGCCCTCCTGGAGCTACAT CAGGAGAAACTAAAAGAGGAGAAGAAGAAGAAAAAGAAGAAGAAGAAACACCGCAAGAGCAGTTCTGACA GCGACGATGAGGAACGGAAGCAGGAGAAACTGAAAAAGGCACTGAATGCAGAGGAGGCTCGCCTTCTTCA CGTGAAGGAGATCATGCAGATTGACGAGCGGAAGCGGCCCTACAACAGCATCTATGAAACCCGAGAGCCC ACAGAAGAGGAGATGGAGGCCTACAGGATGAAACGGCAGAGGCCCGATGACCCCATGGCCTCTTTCCTAG GACAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_148673
Insert Size	1758 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_148673.3, NP_683514.2
RefSeq Size	3732 bp
RefSeq ORF	1758 bp
Locus ID	193116
UniProt ID	Q8BHJ9
Gene Summary	Pre-mRNA splicing occurs in two sequential transesterification steps. The protein encoded by this gene is a splicing factor that has been found to be essential during the second catalytic step in the pre-mRNA splicing process. It associates with the spliceosome and contains a zinc knuckle motif that is found in other splicing factors and is involved in protein-nucleic acid and protein-protein interactions. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) is the longer transcript. Variants 1 and 2 encode the same protein.

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