Elf2 (NM_001291059) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2610036A20Rik; A230104O07Rik; AW111824; BB183398; EU32; NERF; NERF-1A; NERF-1B; NERF-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228370 representing NM_001291059 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATCGGCGGTGGTTGACAGCGGAGGCTCTGCTCTGGAGCTTCCTAGCGATGGAGGAGAAAATCAAG AGGGAGGTGACACGGGCCCTGACTGCCCGGCAGTGATTGTGGAGCCAGTTCCCAGTGCGAGGTTAGAGCA GGGCTATGCTGCCCAGGTGCTGGTCTATGACGACGAGACTTATATGATGCAGGATGTGGCGGAAGAACAG GAAGTTGAGACGGAGAATTCAGAAACAGTGGAAGCATCAGTTCACAGCAGTAATGCTCACTGTACGGATA AGACCATTGAAGCTGCTGAAGCCCTGCTTCATATGGAATCTCCTACCTGCTTGAGGGATTCAAGAAGTCC TGAGTTTATCCATGCTGCGATGAGGCCGGATGTCATCACAGAGACTGTAGTGGAGGTGTCCACAGAAGAG TCTGAGCCGATGGATGCCTCGCCTATCCCTACTTCACCAGACAGCCACGAACCAATGAAAAAGAAAAAAG TTGGCCGTAAACCAAAGACCCAGCAGTCACCAGTTTCCAATGGGTCTCCTGAGTTAGGAATCAAGAAGAA AGCCAGAGAAGGAAAAGGAAACACAACCTATCTGTGGGAGTTTCTTTTAGATTTGCTTCAAGATAAAAAT ACTTGTCCCAGGTATATTAAATGGACTCAGAGAGAAAAGGGCATATTCAAACTTGTGGACTCAAAGGCTG TCTCGAAGCTCTGGGGGAAGCATAAGAACAAACCAGATATGAACTATGAAACCATGGGGCGAGCGTTAAG ATATTACTACCAAAGGGGAATTCTTGCAAAGGTTGAAGGACAGAGGCTTGTATATCAGTTCAAGGACATG CCCAAAAACATAGTGGTCATTGATGATGACAAAAGTGAGACCTGCCCTGAAGACTTGGCAGCAGCTGCTG ATGACAAGTCTTTAGAACGAGTGTCACTATCTGCAGAAAGTCTCCTGAAGGCAGCAACTGCCGTCCGAGG TGGGAAAAACTCATCTCCTCTGAACTGCTCCAGAGCAGAGAAGGGTGTGGCTAGAGTTGTGAACATCACC TCCCCTACTCACGACGGTTCCTCCAGGTCTCCTACTACCACTGCACCTGTGTCAGCAGCAGCAGCTCCAA GGACAGTTCGTGTGGCAATGCAAGTCCCTGTGGTAATGACCTCACTGGGCCAAAAGATTTCAGCGGTGGC AGTTCAGTCAGTCAATGCAGGCACAGGCTCGCCGTTAATAACCAGCACCAGTCCAGCCTCGGCCAGCTCT CCAAAAGTAGTCATCCAGACAGTCCCGACCGTGATGCCGGCCTCCACTGAAAATGGAGACAGAATCACCA TGCAGCCTGCCAAGATTATCACCATCCCCGCCACCCAGCTCGCACAGTGTCAACTCCAGGCAAAGTCAAA CCTGACGGGGTCAGGAAGCATTAACATTGTTGGAACCCCACTGGCTGTGAGGGCACTCACCCCTGTTTCA ATAGCCCACGGTACACCTGTAATGAGACTGTCTGTGCCTGCTCAGCAGGCTTCTGGCCAGACTCCTCCTC GAGTTATCAGTGCGCTCCTAAAAGGGCCGGAGGGGAAATCAGAAGCCAAAAAGCAGGAACACGATGTGAA AACTTTGCAGCTGGTAGAAGAGAAGGGGGCAGACGGCAATAAGACAGTAACCCACGTAGTGGTCGTCAGT GCGCCGTCTGCTATCGCCCTTCCTGTGACTATGAAAACCGAAGGGCTAGTGACGTGTGAGAAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291059
Insert Size	1746 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291059.1, NP_001277988.1
RefSeq Size	5864 bp
RefSeq ORF	1746 bp
Locus ID	69257
UniProt ID	Q9JHC9
Gene Summary	Probably transcriptionally activates the LYN and BLK promoters and acts synergistically with RUNX1 to transactivate the BLK promoter.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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