Grk3 (NM_001285806) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4833444A01Rik; Adrbk-2; Adrbk2; AI851927; AW551196; Bark-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228367 representing NM_001285806 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACGACGCCTACATCATGAGGGAGCTCCTATCCAGCACACACCAATTCTCAAAGCAAGCTGTAGAAC ATGTCCAGAGCCATCTCTCCAAGAAACAGGTGACGGCTACACTTTTCCAGCCATACATAGAAGAAATCTG TGAAAGCCTTCGTGGGGATATTTTCCAAAAGTTTATGGAAAGCGATAAATTCACTAGATTCTGTCAGTGG AAGAACGTGGAGTTGAATATTCACCTGAGCATGAACGACTTCAGTGTGCACAGGATCATCGGCCGAGGAG GGTTTGGGGAAGTTTATGGCTGCAGGAAAGCAGACACCGGTAAAATGTATGCCATGAAGTGCTTAGACAA GAAAAGGGTGAAGATGAAGCAGGGGGAGACTCTGGCTTTGAACGAGAGGATCATGCTGTCTCTCGTTAGC ACTGGGGATTGTCCCTTTATTGTCTGCATGACCTACGCCTTCCACACGCCGGACAAACTCTGCTTCATCC TGGACCTGATGAACGGGGGCGACATGCACTACCATCTCTCTCAACACGGGGTGTTTTCTGAGAAGGAGAT GCGGTTTTATGCCAGCGAGATCATCCTGGGCCTCGAGCACATGCACACCTGCTTCGTAGTCTACAGAGAC CTGAAGCCTGCGAACATCCTCCTAGATGAATATGGGCACGTGAGGATATCGGATCTCGGCCTTGCCTGCG ATTTCTCCAAAAAGAAGCCTCATGCCAGCGTGGGCACCCATGGGTACATGGCTCCCGAGGTGTTGCAGAA GGGAACGTGCTATGACAGCAGCGCCGACTGGTTCTCCCTGGGCTGTATGCTCTTCAAGCTTCTGCGGGGC CACAGCCCCTTCAGGCAGCATAAAACCAAAGACAAGCATGAGATAGACCGAATGACCCTGACCGTGAACG TGCAGCTTCCAGATGCCTTCTCCCCTGAGCTGAGGTCCCTCTTAGAGGGTTTGCTCCAGCGGGACGTGAG CCAGCGGCTGGGCTGCGGAGGAGGAGGGGCACGAGAGTTGAAGGAGCACATCTTCTTCAAGGGCATTGAC TGGCAGCATGTGTACTTACGGAAGTACCCGCCACCCCTAATCCCTCCTCGGGGAGAGGTCAACGCTGCAG ATGCCTTCGATATCGGCTCCTTCGATGAGGAAGACACCAAAGGCATTAAGCTGTTGGACTGTGACCAGGA CCTCTATAAGAACTTCCCACTGGTGATCTCCGAGCGCTGGCAGCAAGAAGTGGTGGAGACCATCTATGAC GCCGTCAATGCTGATACTGATAAAATCGAGGCCAGGAGGAAGGCTAAAAATAAGCAACTTGGTCAAGAGG AAGATTACGCTATGGGGAAGGACTGCATCATGCACGGGTACATGCTGAAGCTGGGGAACCCCTTTCTCAC ACAGTGGCAAAGACGCTATTTTTACCTGTTCCCCAACAGACTGGAGTGGAGAGGAGAGGGCGAGTCTCGG CAAAGTCTACTGACCATGGAACAGATCATGTCTGTGGAGGAGACCCAGATTAAAGACAGAAAGTGCATCT TACTCAGGATAAAGGGAGGGAAGCAATTTGTCTTGCAATGTGAGAGTGACCCCGAGTTTGCACAGTGGCT GAAGGAGCTGACCTGCACCTTCAATGAGGCCCAGAGACTGCTGCGCCGTGCCCCCAAATTCCTCAACAAA CCACGGGCCGCCATCCTGGAGTTCTCCAAGCCACCACTGTGTCACAGAAATAGCAGCGGCCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001285806
Insert Size	1746 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001285806.1, NP_001272735.1
RefSeq Size	6617 bp
RefSeq ORF	1746 bp
Locus ID	320129
UniProt ID	Q3UYH7
Gene Summary	Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses an alternate splice site in the 5' region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 3, which is shorter at the N-terminus, compared to isoform 1.

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