Fut8 (NM_001252614) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228348 representing NM_001252614 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGCATGGACTGGTTCCTGGCGTTGGATTATGCTCATTCTTTTTGCCTGGGGGACCTTGTTATTTT ATATAGGTGGTCATTTGGTTCGAGATAATGACCACCCTGATCACTCCAGCAGAGAACTCTCCAAGATTCT TGCAAAGCTTGAACGCTTAAAACAGCAAAATGAAGACTTGAGGCGAATGGCTGAGTCTCTCCGAATACCA GAAGGCCCCATTGACCAGGGGACAGCTACAGGAAGAGTCCGTGTTTTAGAAGAACAGCTTGTTAAGGCCA AAGAACAGATTGAAAATTACAAGAAACAAGCTAGAAATGGTCTGGGGAAGGATCATGAAATCTTAAGAAG GAGGATTGAAAATGGAGCTAAAGAGCTCTGGTTTTTTCTACAAAGCGAACTGAAGAAATTAAAGCATTTA GAAGGAAATGAACTCCAAAGACATGCAGATGAAATTCTTTTGGATTTAGGACACCATGAAAGGTCTATCA TGACAGATCTATACTACCTCAGTCAAACAGATGGAGCAGGGGATTGGCGTGAAAAAGAGGCCAAAGATCT GACAGAGCTGGTCCAGCGGAGAATAACATATCTCCAGAATCCTAAGGACTGCAGCAAAGCCAGGAAGCTG GTGTGTAACATCAATAAAGGCTGTGGCTATGGTTGTCAACTCCATCACGTGGTCTACTGTTTCATGATTG CTTATGGCACCCAGCGAACACTCATCTTGGAATCTCAGAATTGGCGCTATGCTACTGGTGGATGGGAGAC TGTGTTTAGACCTGTAAGTGAGACATGTACAGACAGATCTGGCCTCTCCACTGGACACTGGTCAGGTGAA GTAAATGACAAAAACATTCAAGTGGTCGAGCTCCCCATTGTAGACAGCCTCCATCCTCGGCCTCCTTACT TACCACTGGCTGTTCCAGAAGACCTTGCAGACCGACTCCTAAGAGTCCATGGTGACCCTGCAGTGTGGTG GGTGTCCCAGTTTGTCAAATACTTGATTCGTCCACAACCTTGGCTGGAAAAGGAAATAGAAGAAGCCACC AAGAAGCTTGGCTTCAAACATCCAGTTATTGGAGTCCATGTCAGACGCACAGACAAAGTGGGAACAGAAG CAGCCTTCCACCCCATCGAGGAGTACATGGTACACGTTGAAGAACATTTTCAGCTTCTCGCACGCAGAAT GCAAGTGGATAAAAAAAGAGTATATCTGGCTACTGATGATCCTACTTTGTTAAAGGAGGCAAAGACAAAG TACTCCAATTATGAATTTATTAGTGATAACTCTATTTCTTGGTCAGCTGGACTACACAATCGGTACACAG AAAATTCACTTCGGGGTGTGATCCTGGATATACACTTTCTCTCACAGGCTGACTTTCTAGTGTGTACTTT TTCATCCCAGGTCTGTCGGGTTGCTTATGAAATCATGCAAACCCTGCATCCTGATGCCTCTGCGAACTTC CATTCTTTGGATGACATCTACTATTTTGGAGGCCAAAATGCCCACAATCAGATTGCTGTTTATCCTCACA AACCTCGAACTGAAGAGGAAATTCCAATGGAACCTGGAGATATCATTGGTGTGGCTGGAAACCATTGGGA TGGTTATTCTAAAGGTATCAACAGAAAACTTGGAAAAACAGGCTTATATCCCTCCTACAAAGTCCGAGAG AAGATAGAAACAGTCAAGTATCCCACATATCCTGAAGCTGAAAAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252614
Insert Size	1728 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252614.1, NP_001239543.1
RefSeq Size	3012 bp
RefSeq ORF	1728 bp
Locus ID	53618
UniProt ID	Q9WTS2
Gene Summary	Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has an additional exon in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same isoform. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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