Impdh1 (NM_001302934) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	B930086D20Rik; IMPDH-I
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228339 representing NM_001302934 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAACCGCTCTCACCCCCACCGGAGTCCGGGCTGCTCTCGCCGCTGCTGCAGGGAGGTGGAGCGG CTGCCGCTCCAGAGCCCGGAGCCCGGCAACACCCGGGACATGAGACCGCGGCGCAGCGGTACAGCGCCCG CCTGCTGCAGGCCGGCTACGAGCCGGAGAGCATGGCGGACTACCTGATCAGCGGCGGCACCGGCTACGTT CCCGAGGATGGGCTCACCGCGCAGCAGCTCTTTGCCAACGCGGATGGCCTCACCTACAACGACTTCCTGA TCCTCCCAGGATTCATAGACTTCATAGCTGATGAAGTGGACCTGACATCAGCCCTGACCCGGAAGATCAC ACTGAAGACACCATTGATCTCGTCTCCCATGGATACAGTGACAGAGGCTGATATGGCCATTGCAATGGCT CTCATGGGAGGAATTGGTTTCATTCATCACAACTGTACCCCAGAGTTCCAGGCCAATGAAGTACGGAAGG TCAAGAAGTTTGAGCAAGGCTTCATCACAGACCCTGTGGTCCTGAGCCCTTCACATACTGTGGGTGATGT TCTGGAGGCCAAGATACAGCATGGCTTCTCTGGTATCCCCATCACCGCGACGGGCACCATGGGGAGCAAG CTGGTGGGCATCGTCACCTCCCGAGACATTGACTTCCTTGCTGAGAAGGACCACACCACCCTCCTCAGTG AGGTGATGACTCCGAGGGTCGAGCTGGTGGTGGCTCCAGCAGGTGTGACATTGAAAGAAGCAAATGAGAT CTTGCAGCGCAGCAAGAAAGGGAAGCTGCCCATAGTCAACGATCAAGATGAGCTGGTAGCCATCATTGCG CGCACAGACCTGAAGAAGAACAGAGACTACCCTCTGGCCTCCAAGGACTCCCACAAACAGCTGTTGTGTG GGGCAGCTGTGGGCACCCGTGAGGATGACAAATACCGCCTGGACCTGCTCACTCAGGCCGGTGCTGACGT CATAGTACTAGATTCATCCCAGGGGAACTCAGTGTATCAGATCGCCATGGTGCACTATATCAAGCAGAAG TACCCCCACCTCCAAGTGATTGGGGGAAATGTGGTGACAGCAGCCCAGGCCAAGAACTTGATTGATGCTG GTGTGGACGGGCTTCGTGTGGGCATGGGCTGTGGTTCCATCTGCATCACCCAGGAAGTGATGGCCTGTGG CCGACCCCAGGGGACTGCTGTCTACAAGGTGGCCGAGTACGCCCGACGTTTTGGGGTGCCGGTAATAGCG GATGGTGGCATCCAGACCGTGGGCCATGTGGTCAAAGCCCTGGCACTTGGAGCCTCTACAGTAATGATGG GCTCCCTGCTGGCTGCCACCACGGAGGCGCCTGGTGAATACTTCTTCTCAGATGGGGTGAGGCTGAAGAA GTACCGGGGCATGGGTTCTCTGGACGCCATGGAGAAGAGCAGCAGCAGCCAGAAAAGATACTTCAGTGAG GGGGATAAGGTGAAGATCGCACAAGGTGTCTCCGGTTCCATCCAGGATAAAGGCTCCATTCAGAAGTTTG TGCCCTACCTCATAGCAGGGATCCAGCATGGCTGCCAGGATATTGGGGCCCAAAGCCTATCTGTCCTGCG ATCCATGATGTACTCAGGAGAGCTCAAGTTTGAGAAGCGGACCATGTCGGCCCAGATTGAGGGTGGCGTG CACGGCCTACACTCTTACGAGAAGCGGCTGTACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302934
Insert Size	1716 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302934.1, NP_001289863.1
RefSeq Size	2617 bp
RefSeq ORF	1716 bp
Locus ID	23917
UniProt ID	P50096
Gene Summary	Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform 2 which is shorter by one amino acid compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.