Arntl2 (NM_001289680) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4632430A05Rik; bHLHe6; BMAL2; CLIF; MOP9
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228337 representing NM_001289680 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTTCCAAGGAAACGCAGAGGCAGAGATTCCCAGCCACTCCAGTCAGAATTCATGACAGACACAA CAGTGGAAAGTCTTCCCCAGAATCCCTTTGCCTCTCTTCTTTCAACAAGAACAGGAGTATCAGCGCCCAG TGGCATCAGGGAAGCTCACAGCCAGATGGAAAAGCGTCGGAGAGACAAGATGAACCATCTGATTCAGAAA CTGTCATCTATGATCCCTCCACACATCCCCACGGCCCACAAACTGGACAAGCTCAGCGTCTTGAGGAGGG CGGTGCAGTACTTGAGGTCTCTGAGAGGCATGACAGAGCTTTACTTAGGAGAAAACTCTAAACCTTCATT TATTCAGGATAAGGAACTCAGTCACTTAATCCTCAAGGCAGCAGAAGGCTTCCTGTTTGTGGTTGGATGC GAAAGAGGGAGAATTTTTTACGTTTCTAAGTCTGTCTCCAAAACACTGCGTTATGATCAGGCTAGCTTGA TAGGACAGAATTTGTTTGACTTCTTACACCCAAAAGACGTCGCCAAAGTAAAGGAACAACTTTCTTGTGA TGGTTCACCAAGAGAGAAACCTATAGACACCAAAACCTCTCAGGTTTACAGTCACCCCTACACTGGGCGA CCACGCATGCATTCTGGCTCCAGACGATCTTTCTTCTTTAGAATGAAGAGCTGTACCGTCCCTGTCAAAG AAGAGCAGCCATGCTCGTCCTGCTCAAAGAAGAAAGACCATAGAAAATTCCACACCGTCCATTGCACTGG ATACTTGAGAAGCTGGCCTCTGAATGTTGTTGGCATGGAGAAAGAGTCGGGTGGTGGGAAGGACAGCGGT CCTCTTACCTGCCTTGTGGCTATGGGACGGTTGCATCCATACATTGTCCCTCAAAAGAGTGGCAAGATCA ACGTGAGACCGGCTGAGTTCATAACTCGCTTCGCAATGAACGGGAAATTCGTCTATGTTGACCAAAGGGC AACGGCAATTTTAGGATACCTGCCTCAGGAACTTTTGGGAACTTCATGTTATGAATATTTTCATCAGGAT GACCACAGTAGTTTGACTGACAAGCACAAAGCAGTTCTGCAGAGTAAGGAGAAAATACTTACAGACTCAT ACAAATTCAGAGTGAAGGATGGTGCCTTCGTGACTCTGAAGAGTGAGTGGTTCAGCTTCACAAACCCTTG GACCAAAGAGCTGGAGTACATTGTGTCTGTCAACACATTGGTTTTGGGGCGCAGTGAGACCAGGCTGTCT TTGCTTCATTGCGGCGGCAGCAGCCAGTCCTCCGAAGACTCATTTAGACAATCCTGCATCAATGTGCCCG GTGTATCCACGGGGACCGTCCTTGGTGCTGGGAGTATTGGAACAGATATTGCAAATGAGGTTCTGAGTTT ACAGAGATTACACTCTTCATCCCCAGAAGATGCAAGCCCTTCAGAAGAAGTGAGAGATGACTGCAGTGCA TCCACTAGGGAGCCTTTTGCAGTGAGCCCTTCTGAAACAGAGGTCCTGGAGGCTGCCAGGCAACACCAGA GCACTGAACCCGCCCACCCTCACGGACCACTTCCCGGTGACAGTGCCCAGCTGGGTTTTGATGTCCTGTG TGACAGTGACAGCATAGACATGGCTGCATTCATGAATTACCTCGAAGCAGAGGGGGGCCTGGGTGACCCT GGGGACTTCAGTGACATCCAGTGGGCACTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289680
Insert Size	1713 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289680.1, NP_001276609.1
RefSeq Size	2596 bp
RefSeq ORF	1713 bp
Locus ID	272322
UniProt ID	Q2VPD4
Gene Summary	This gene encodes a basic helix-loop-helix transcription factor belonging to the PAS (Per, Arnt, Sim) superfamily. The PAS proteins play important roles in adaptation to low atmospheric and cellular oxygen levels, exposure to certain environmental pollutants, and diurnal oscillations in light and temperature. This protein forms a transcriptionally active heterodimer with the circadian Clock protein, the structurally related Mop4, and hypoxia-inducible factors, such as Hif1alpha. Consistent with its role as a biologically relevant partner of circadian and hypoxia factors, this protein is coexpressed in regions of the brain such as the thalamus, hypothalamus, and amygdala. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2014] Transcript Variant: This variant (3) uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (3) is shorter than isoform 1.

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