Zyx (NM_001289617) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9530098H06Rik; R75157
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228322 representing NM_001289617 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCCCCCGCCCGCCTCCCGCGATCTCCGTCTCCGTCTCGGCCCCCGCGTTTTACGCCCCGCAGA AGAAGTTCGCCCCGGTTGTGGCCCCAAAGCCCAAAGTGAATCCTTTCCGGCCTGGGGACAGCGAGCCTCC TGTAGCAGCCGGGGCCCAAAGAGCGCAGATGGGTCGGGTGGGCGAGATCCCACCACCACCCCCGGAAGAC TTTCCTTTGCCCCCTCCTCCCCTTATTGGGGAGGGCGACGACTCAGAGGGTGCCCTGGGAGGTGCCTTCC CACCTCCACCTCCCCCGATGATCGAGGAACCATTCCCCCCTGCTCCTCTGGAGGAGGACATCTTCCCCTC CCCTCCACCTCCACTGGAGGAGGAGGGAGGGCCTGAGGCCCCTACCCAGCTCCCACCGCAGCCCAGGGAG AAAGTGTGCAGTATTGACCTGGAGATTGACTCTCTGTCCTCACTGCTGGACGACATGACCAAGAACGATC CCTTCAAAGCCCGGGTATCATCCGGATATGTACCCCCACCAGTTGCCACTCCATTTGTTCCCAAGCCTAG TACCAAACCTGCCCCTGGGGGCACAGCACCCTTGCCTCCTTGGAAGACCCCTTCTAGCTCCCAGCCACCA CCTCAGCCGCAGGCCAAGCCTCAGGTCCAGCTCCATGTCCAGCCTCAGGCCAAGCCCCATGTCCAACCCC AGCCTGTGTCTTCTGCTAATACACAGCCCCGGGGTCCCCTTTCTCAGGCACCAACTCCAGCACCTAAGTT TGCTCCAGTGGCTCCTAAATTTACTCCCGTGGTTTCCAAGTTCAGCCCTGGTGCTCCAAGTGGACCTGGG CCACAGCCCAATCAAAAAATGGTGCCTCCGGATGCTCCTTCTTCTGTGAGCACAGGCTCCCCTCAGCCCC CTAGCTTCACCTATGCTCAGCAGAAGGAGAAGCCCCTAGTTCAAGAGAAGCAGCACCCACAGCCTCCACC AGCTCAAAACCAAAACCAGGTACGCTCTCCTGGAGGCCCAGGCCCCTTGACCCTGAAGGAGGTAGAGGAG TTGGAGCAGCTGACCCAGCAGCTGATGCAGGACATGGAACACCCTCAGAGGCAGAGCGTGGCAGTGAATG AGTCCTGTGGCAAATGCAATCAGCCACTGGCCCGTGCACAGCCTGCGGTTCGTGCACTGGGACAACTGTT CCACATCACCTGCTTCACTTGCCATCAGTGTCAGCAGCAGCTGCAGGGACAGCAGTTCTATAGCCTGGAG GGAGCACCATATTGTGAGGGCTGCTACACCGACACTTTGGAGAAGTGCAACACCTGTGGGCAGCCCATCA CTGACCGCATGCTGAGGGCCACTGGCAAAGCCTACCACCCACAGTGCTTCACCTGTGTGGTCTGCGCCTG TCCCCTGGAGGGCACCTCCTTCATTGTGGACCAGGCCAATCAGCCCCACTGTGTCCCTGACTATCACAAG CAATACGCTCCAAGGTGCTCCGTCTGCTCGGAGCCAATCATGCCTGAGCCTGGCCGAGACGAGACTGTGC GAGTAGTGGCGCTGGATAAGAACTTTCATATGAAGTGTTACAAGTGTGAGGACTGTGGGAAACCTCTGTC CATTGAGGCAGATGACAACGGCTGTTTCCCTCTGGATGGCCACGTCCTTTGTCGGAAGTGCCACTCCGCT AGAGCCCAGACCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289617
Insert Size	1695 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289617.1, NP_001276546.1
RefSeq Size	3412 bp
RefSeq ORF	1695 bp
Locus ID	22793
UniProt ID	Q62523
Gene Summary	Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 3' UTR, compared to variant 1. Variants 1 and 2 encode the same isoform (a).

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