Cpeb1 (NM_001252525) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AU024112; Cpe-bp1; Cpeb; mCPEB; mCpeb-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228318 representing NM_001252525 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTTCTCTCTGGAAGAAGCAGCAGGAAGAATAAAGGATTGCTGGGACAACCAAGAAGTTCCTGCAC TCTCTACATGTAGCAATGCCAATATCTTTCGAAGGATAAATGCCATTTTGGATGATTCACTGGACTTCAG TAAAGTCTGTACAACACCTATAAATCGAGGAATTCATGATCAGTTGCCAGCAGACTTCCAGGACTCTGAA GAAACAGTTACAAGCAGGATGCTTTTCCCCACCTCTGCGCAAGAATCTCCCCGTGGCCTCCCAGATGCAA ATGGCTTGTGCCTTGGCCTGCAGTCCCTCAGTCTGACTGGCTGGGACCGACCCTGGAGCACCCAGGACTC AGACTCCTCAGCCCAGAGCAGCACACAGTCAGTATTAAGCATGCTCCAAAATCCACTGGGAAATGTCTTA GGAAAAGCCCCCTTGAGCTTCCTGTCTCTGGATCCCCTTGGGTCTGACTTGGACAAGTTCCCAGCACCCT CAGTTAGAGGATCTCGCCTGGACACCCGGCCCATCCTGGACTCCCGTTCTAGCAGCCCCTCTGACTCAGA CACGAGTGGCTTCAGCTCTGGATCAGACCATCTCTCAGATTTGATTTCAAGCCTTCGCATTTCCCCTCCT CTGCCCTTTCTTTCTATGACGGGAAATGGTCCCAGAGACCCTCTAAAGATGGGCGTTGGGTCCCGAATGG ACCAAGAACAAGCTGCTCTTGCTGCAGTTGCTCCCTCTCCAACCAGTGCTCCAAAGAGATGGCCAGGAGC TTCTGTGTGGCCATCTTGGGACCTTCTTGGAGCTCCTAAAGACCCATTCAGCATAGAGAGAGAGGCTAGA CTCCACCGGCAGGCTGCAGCTGTGAATGAAGCTACGTGTACCTGGAGTGGCCAGCTTCCTCCCCGGAACT ATAAGAACCCCATCTACTCATGCAAGGTGTTCCTAGGAGGCGTTCCTTGGGATATTACAGAAGCTGGATT GGTTAACACCTTCCGTGTTTTTGGCTCTCTGAGTGTGGAGTGGCCTGGTAAGGATGGCAAGCACCCCCGG TGTCCTCCCAAAGGTAATATGCCTAAAGGGTATGTGTATTTGGTGTTTGAACTAGAGAAGTCCGTCCGGG CCTTGCTTCAGGCTTGTTCTCATGACCCGCTGAGCCCAGATGGCCTGAGTGAATATTATTTCAAGATGTC CAGCCGAAGGATGCGCTGCAAAGAGGTACAGGTAATCCCTTGGGTCTTGGCTGACAGCAACTTTGTCTGG AGCCCATCTCAGAGGCTGGATCCCAGCAGGACGGTGTTTGTTGGTGCCTTGCACGGGATGCTCAATGCTG AGGCTCTGGCTGCCATCTTGAATGACCTATTTGGTGGAGTGGTGTATGCTGGGATTGACACAGATAAGCA CAAGTACCCCATTGGGTCTGGTCGTGTGACTTTCAATAACCAACGTAGTTATCTGAAAGCAGTCACCGCT GCTTTTGTGGAGATCAAAACCACCAAGTTTACCAAGAAGGTTCAGATTGACCCCTATCTAGAAGATTCTC TGTGTCTTATCTGCAGCTCACAACCTGGTCCTTTCTTCTGCCGAGATCAGGTCTGCTTCAAGTACTTTTG CAGAAGCTGCTGGCACTGGCGACACAGCATGGAAGGCCTACGCCACCATAGCCCCCTAATGAGGAACCAG AAGAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252525
Insert Size	1689 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252525.1, NP_001239454.1
RefSeq Size	3141 bp
RefSeq ORF	1689 bp
Locus ID	12877
UniProt ID	P70166
Gene Summary	Sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the 3' UTR of mRNAs. In absence of phosphorylation and in association with TACC3 is also involved as a repressor of translation of CPE-containing mRNA; a repression that is relieved by phosphorylation or degradation (By similarity). Involved in the transport of CPE-containing mRNA to dendrites; those mRNAs may be transported to dendrites in a translationally dormant form and translationally activated at synapses. Its interaction with APLP1 promotes local CPE-containing mRNA polyadenylation and translation activation. Induces the assembly of stress granules in the absence of stress (By similarity). Required for cell cycle progression, specifically for prophase entry (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and it encodes the longest protein (isoform 1).

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