Syncrip (NM_001284328) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2610109K23Rik; 4632417O19Rik; GRY-RBP; hnRNP Q; Nsap1; Nsap1l; pp68
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228314 representing NM_001284328 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTACAGAACATGTTAATGGAAATGGTACTGAAGAGCCCATGGATACTACTTCAGCAGTTATCCATT CAGAAAATTTTCAGACATTGCTTGATGCTGGTTTACCACAGAAAGTTGCTGAAAAACTAGATGAAATTTA CGTTGCAGGGCTAGTTGCACATAGTGATTTAGATGAAAGAGCTATCGAAGCTTTAAAAGAGTTCAATGAA GACGGCGCATTGGCAGTGCTTCAACAGTTTAAAGACAGTGATCTCTCTCATGTTCAGAACAAAAGTGCCT TTTTATGTGGAGTCATGAAGACTTACAGGCAGAGAGAAAAACAGGGGACCAAAGTAGCAGACTCTAGTAA AGGACCAGATGAGGCAAAGATTAAGGCACTTTTGGAAAGAACAGGCTACACACTTGATGTGACTACAGGT CAGAGGAAGTATGGAGGACCACCTCCAGATTCCGTTTATTCAGGTCAGCAGCCTTCTGTTGGCACTGAGA TATTTGTGGGGAAGATCCCCAGAGATCTGTTTGAGGATGAGCTTGTTCCATTATTTGAGAAAGCTGGACC TATATGGGATCTTCGTTTAATGATGGATCCGCTCACTGGTCTCAACAGAGGTTATGCGTTTGTCACTTTT TGTACAAAAGAAGCAGCACAAGAGGCTGTTAAACTGTATAATAATCATGAAATTCGTTCCGGGAAGCACA TTGGTGTCTGCATCTCAGTTGCCAACAATAGGCTTTTTGTGGGCTCGATTCCTAAGAGTAAAACCAAGGA GCAGATTCTTGAGGAATTTAGCAAAGTGACAGAGGGTCTCACAGATGTCATTTTATACCACCAACCTGAT GACAAGAAAAAAAACAGAGGCTTTTGCTTTCTTGAATATGAAGATCACAAAACAGCTGCCCAGGCAAGAC GTAGGCTAATGAGTGGTAAAGTCAAAGTCTGGGGAAATGTTGGAACTGTTGAGTGGGCTGATCCTATTGA AGATCCTGATCCTGAAGTTATGGCAAAGGTAAAAGTGCTGTTTGTACGCAACCTTGCCAACACGGTAACA GAAGAAATTTTAGAAAAGTCATTTAGTCAGTTTGGGAAACTGGAACGAGTAAAGAAGCTAAAAGATTATG CTTTCATTCATTTTGATGAGAGAGATGGTGCTGTCAAGGCTATGGAAGAAATGAATGGTAAAGACTTGGA GGGAGAAAATATTGAAATTGTTTTTGCTAAGCCACCAGATCAGAAGAGGAAAGAAAGAAAAGCTCAGAGG CAAGCAGCAAAGAATCAAATGTATGATGATTACTACTATTATGGTCCACCTCATATGCCTCCCCCAACAA GAGGTCGAGGGCGTGGAGGTAGAGGTGGCTATGGATATCCTCCAGATTATTATGGATACGAAGATTATTA TGATTATTATGGTTATGATTACCATAACTATCGTGGTGGATATGAAGATCCATACTATGGTTATGAAGAT TTTCAAGTTGGAGCTAGAGGAAGGGGTGGTAGAGGAGCAAGGGGTGCTGCTCCATCCAGAGGTCGTGGGG CTGCTCCTCCCCGTGGTAGAGCCGGTTATTCACAGAGAGGAGGCCCTGGATCAGCAAGAGGCGTTCGCGG TGCGAGAGGAGGTGCCCAACAACAAAGAGGCCGCGGGGGAAAAGGGGTCGAGGCCGGTCCTGACCTGTTA CAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001284328
Insert Size	1686 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001284328.1, NP_001271257.1
RefSeq Size	6930 bp
RefSeq ORF	1686 bp
Locus ID	56403
UniProt ID	Q7TMK9
Gene Summary	Heterogeneous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD-mediated complex that promotes MYC mRNA stability. Isoform 1 and isoform 2 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences (By similarity). Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself (By similarity). May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain (By similarity). Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 2 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses a distinct 3' terminal exon and differs in the 3' coding region and 3' UTR, compared to variant 1. The resulting protein (isoform 3) is shorter and has a distinct C-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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