Tiam2 (NM_001286758) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	3000002F19Rik; mKIAA2016; STEF
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228288 representing NM_001286758 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCACTTTTTGGGAGCCTGCCAGAGATGCTGGAGTTTCAAAAGGTGTTCCTGGAGACGTTGGAGG ATGCGATCTCCGCTTCCTCGGACTTTAGTGTCCTGGAAACCCCCTCACAGTTTCGAAAATTGCTGTTCTC CCTTGGAGGTTCTTTCCTCTACTATGCGGATCACTTTAAGCTATACAGTGGGTTCTGTGCCAACCACATT AAAGTACAGAGGGTTCTAGAGCGAGCTAAAACGGACAAGGCCTTCAAGGCTTTTCTGGATGCCCGAAATC CCACCAAGCAGCACTCCTCCACGCTGGAGTCCTATCTCATCAAGCCTGTTCAGAGAGTGCTCAAGTATCC TCTGCTTCTCAAGGAGCTAGTGTCACTGACTGACCATGAGAGTGAAGAACACTATCACCTGACAGAAGCA CTAAAGGCCATGGAAAAAGTCGCCAGTCACATCAATGAGATGCAGAAGATCTACGAGGATTACGGGATGG TGTTTGACCAGCTGGTGGCAGAGCAGAGTGGCACAGAGAAGGAAGTGACAGAGCTGTCCATGGGGGAACT TCTGATGCACTCTACAGTTTCCTGGTTGAATCCGTTCCTGTCTCTAGGAAAAGCCAGGAAGGACATTGAG CTCACAGTATTTGTTTTTAAGAGAGCTGTCATACTGGTTTATAAAGAAAACTGCAAGCTGAAAAAGAAAC TGCCCTCGAATTCCCGGCCTGCTCACAACTCTGCTGACTTGGATCCATTTAAATTCCGCTGGTTGATTCC CATATCTGCGCTTCAAGTTAGACTGGGGAACACGGCAGGGACTGAAAATAATTCCACGTGGGAGCTGATT CATACCAAGTCGGAAATTGAAGGACGGCCAGAAACCATCTTTCAACTGTGCTGCAGTGACAGCGAGAACA AAACCAGCATTGTTAAGGTGATTCGTTCTATTCTGAGAGAGAACTTCCGGCGCCACATAAAGTGTGAGCT GCCACTGGAGAAGACGTGTAAGGACCGGCTAGTACCTCTTAAGAACCGAGTTCCTGTTTCAGCCAAATTA GCCTCGTCCAGGTCGTTGAAGGGCCTCAGAACATCCTCCAGCAGCGAGTGGCCCAGCGAGCCCAGCAAGG GCAACTCACTGGACTCAGATGAGTGCAGCCTGAGCAGTGGCACCCAGAGTAGCGGCTGCCCCGTAGCCGA GAGCAGGCGAGACTCTAAGAGCACCGAGCTGGAGAAAGACGCTCAGGAGGGCCTGGCGGAGTTTCCAGAT GGTCTTATCAAAGAAAGCGACATTCTGAGTGATGAAGATGAGGACTTCCACCACCCTCTGAAACAGGGTA GCCCTACTAAGGACATTGAGATTCAGTTCCAGAGACTGAAAATCTCTGAGGAATCCGACGTGCACCCAGT TGGGCAGCAGCCTCTCACAGAGTCAGGTGAACAGCCCAAGCTGGTCAGGGGCCATTTTTGCCCCATTAAA CGGAAAGCAAACAGCACCAAGAGGGGCAGAGGAACTTTGCTCAAGGCGCAGACTCGTCACCAGTCCCTGG ACAGCCACCCAGAAACTGCCAGCATTGATCTAAACTTGGTCCTGGAGAGAGAATTCAGTGTCCAGAGCTT AACTTCAGTCGTCAATGAGGAGGGTTTTTATGAAACACAGAGCCATGGCAAATCATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286758
Insert Size	1668 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286758.1, NP_001273687.1
RefSeq Size	2550 bp
RefSeq ORF	1668 bp
Locus ID	24001
UniProt ID	Q6ZPF3
Gene Summary	Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Activates specifically RAC1, but not CDC42 and RHOA. Mediates extracellular laminin signals to activate Rac1, contributing to neurite growth. Involved in lamellipodial formation and advancement of the growth cone of embryonic hippocampal neurons. Promotes migration of neurons in the cerebral cortex. When overexpressed, induces membrane ruffling accompanied by the accumulation of actin filaments along the altered plasma membrane.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus, compared to isoform 1.

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