Papss1 (NM_001289479) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI325286; Asapk; SK1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228287 representing NM_001289479 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCTGGAGGAGTACCTGGTGTGCCACGGCATTCCGTGCTACACTTTGGATGGTGACAACATCCGCC AAGGACTCAATAAGAACCTCGGCTTCAGTCCTGAGGACAGAGAAGAGAACGTTCGCCGCATAGCTGAGGT GGCGAAGCTGTTTGCAGATGCTGGCTTAGTGTGTATCACCAGCTTTATATCGCCTTACACACAGGATCGC AACAACGCAAGGCAGATTCATGAGGGTGCAAGCTTGCCCTTCTTTGAAGTTTTTGTCGATGCTCCTCTGC ATGTCTGTGAGCAGAGGGATGTCAAAGGCCTCTACAAGAAGGCGCGGGCAGGGGAGATAAAAGGCTTCAC TGGCATCGATTCTGAGTATGAGAAACCCGAGGCCCCGGAGCTGGTGCTGAAAACGGATTCCTGTGACGTC AACGACTGCGTCCAGCAGGTTGTGGAGCTTCTTCAGGAACGGGACATCGTCCCTGTGGATGCTTCCTATG AAGTGAAAGAGCTATATGTGCCAGAGAATAAACTTCACTTGGCCAAAACTGATGCAGAAGCCTTACCAGC CCTGAAAATCAATAAAGTGGATATGCAGTGGGTGCAGGTTTTGGCAGAAGGTTGGGCGACTCCTCTGAAC GGCTTCATGAGAGAGAGGGAGTACTTGCAGTGCCTCCATTTCGATTGTCTTCTGGATGGAGGCGTCATCA ACTTATCGGTGCCTATAGTTCTGACAGCTACGCACGAGGATAAGGAGAGGCTGGACGGCTGCACCGCGTT CGCTCTGGTGTATGAGGGCCGCCGCGTGGCCATCCTTCGGAATCCTGAATTTTTTGAGCACCGGAAAGAG GAGCGGTGTGCCAGACAGTGGGGAACAACATGCAAGAACCACCCCTACATCAAGATGGTTCTGGAACAAG GGGATTGGCTGATTGGAGGAGATCTTCAAGTCCTGGACCGGATTTACTGGAATGATGGTCTTGATCAGTA CCGCCTTACCCCGACGGAGCTCAAGCAGAAGTTTAAAGATATGAACGCTGATGCTGTCTTTGCATTTCAG TTGCGCAACCCAGTGCACAACGGGCACGCTCTGTTAATGCAGGATACCCACAAGCAGCTTCTGGAGAGGG GCTACCGGCGCCCTGTCCTGCTCCTCCATCCTCTTGGTGGCTGGACGAAGGATGACGATGTCCCTCTGAT GTGGCGTATGAAGCAGCACGCTGCAGTGCTGGAGGAGGGCATCCTGGATCCTGAAACGACAGTGGTGGCC ATTTTCCCGTCTCCTATGATGTATGCTGGGCCAACCGAGGTCCAGTGGCACTGCAGGGCGCGGATGGTGG CCGGAGCCAATTTTTATATTGTTGGACGAGACCCTGCTGGCATGCCTCATCCGGAGACAGGGAAGGACCT CTATGAGCCAACACATGGTGCCAAAGTGCTGACGATGGCCCCAGGCCTGATTACCTTGGAAATCGTTCCC TTCCGAGTTGCAGCTTACAACAAGAAAAAGAAGCGGATGGACTACTATGACTCTGAGCACCACGAAGACT TCGAGTTTATTTCAGGAACGAGGATGCGCAAGCTGGCACGCGAAGGCCAGAAACCTCCAGAGGGCTTCAT GGCCCCCAAGGCCTGGACTGTGCTGGTAGAGTACTACAAGTCCTTAGAGAAAGCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289479
Insert Size	1668 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289479.1, NP_001276408.1
RefSeq Size	2905 bp
RefSeq ORF	1668 bp
Locus ID	23971
Gene Summary	Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway (PubMed:7493984). Required for normal biosynthesis of sulfated L-selectin ligands in endothelial cells (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (c) is shorter at the N-terminus compared to isoform a.

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