Gtf2h1 (NM_001291075) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	62kDa; AW743425; AW822074; BTF2 p62; C77871; p62
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228274 representing NM_001291075 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACCTTATAGCCATCATGGCGACTTCATCAGAAGAAGTGTTGCTGATTGTGAAGAAGGTTCGTCAGA AGAAGCAGGATGGAGCACTGTACCTCATGGCAGAGAGAATTGCTTGGGCACCTGAAGGCAAAGACAGATT TACAATCAGCCATATGTATGCAGATATTAAATGCCAGAAAATCAGTCCAGAGGGAAAAGCTAAAATACAA CTTCAGCTGGTCCTGCATGCAGGGGACACAACAAACTTCCATTTTTCCAACGAAAGCACGGCAGTGAAAG AACGGGATGCAGTGAAGGACCTCCTTCAGCAGCTGCTGCCCAAGTTCAAGCGGAAAGCTAATAAAGAGCT GGAGGAGAAGAACAGAATGCTCCAAGAAGATCCTGTTTTATTCCAACTCTATAAAGACCTTGTTGTGAGC CAAGTGATCAGTGCTGAGGAATTCTGGGCCAATCGTTTAAATGTGAATGCAACAGATAGTTCTACATCCA GTCACAAGCAGGATGTTGGTATTTCTGCAGCATTTCTGGCTGATGTCCGGCCCCAAACGGATGGCTGTAA TGGTCTGAGATATAATTTAACTTCTGATATCATTGAATCTATATTTAGGACCTATCCAGCAGTAAAAATG AAATATGCAGAAACTGTACCACACAACATGACAGAAAAGGAGTTCTGGACACGCTTTTTCCAGTCCCATT ATTTTCACAGGGACCGACTAAATACAGGATCGAAGGACCTCTTTGCAGAATGTGCCAAAATAGATGAGAA GGGATTAAAAACAATGGTTTCATTAGGAGTGAAAAACCCAATGCTAGATTTGACATCGTTGGAAGATAAG CCATTAGACGAGGGCTATGGCATTTCCTCTGTGCCATCTACTTCCAATTCCAAATCCATAAAGGAGAATA GTAATGCTGCCATCATCAAAAGGTTTAACCACCACAGTGCCATGGTCCTGGCTGCTGGTCTCAGGAAACA ACAAGCACAAAATGGACAGAATGGTGAGCCCAGCAGCGTGGATGGGAATTCTGGGGATACAGACTGCTTT CAGCCAGCAGTCAAAAGGGCAAAGTTGCAGGAGTCCATTGAATATGAAGACTTGGGAAACAACAATTCTG TGAAAACGATTGCACTGAATCTCAAGAAGTCAGACAGGTATTACCATGGTCCTACTCCAATTCAGTCACT GCAGTATGCAACAAGTCAGGACATTATTAACTCTTTTCAAAGTATTAGACAAGAAATGGAAGCTTACACA CCCAAGTTAACTCAGGTTCTCTCAAGCAGTGCTGCTAGTAGCACCATCACAGCCCTGTCACCTGGAGGAG CTCTTATGCAGGGAGGGACACAGCAAGCCGTAAACCAGATGGTGCCAAACGATATTCAGTCTGAATTGAA ACACTTGTATGTGGCTGTTGGGGAACTTCTGCGGCATTTTTGGTCTTGCTTTCCTGTTAATACACCATTC CTAGAAGAAAAGGTAGTGAAAATGAAGAGTAATTTGGAACGGTTCCAAGTTACAAAGCTCTGCCCATTCC AAGAAAAGATTCGGAGGCAGTATTTAAGCACAAATCTGGTAAGTCACATAGAAGAGATGCTGCAGACAGC CTACAACAAGCTCCACGCGTGGCAATCCCGGCGCCTGATGAAGAAGACGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001291075
Insert Size	1662 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291075.1, NP_001278004.1
RefSeq Size	2716 bp
RefSeq ORF	1662 bp
Locus ID	14884
UniProt ID	Q9DBA9
Gene Summary	Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) differs in the 5' UTR and coding sequence compared to variant 3. The resulting isoform (1) has a shorter and distinct N-terminus compared to isoform 3. Variants 1 and 4 both encode the same isoform (1).

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