Nf2 (NM_001252253) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	merlin
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228247 representing NM_001252253 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAATGGAAGGGGAAGGACCTGTTTGATTTGGTGTGCCGGACACTGGGGCTTCGGGAAACCTGGTTCT TTGGACTGCAGTATACAATCAAGGACACGGTGGCCTGGCTCAAAATGGACAAGAAGGTGTTGGATCATGA TGTTTCGAAGGAAGAACCAGTTACCTTTCACTTCCTGGCCAAATTTTATCCTGAAAATGCTGAGGAGGAG CTAGTTCAAGAGATCACGCAACACTTATTTTTCTTACAGGTGAAGAAGCAGATTTTGGATGAAAAGGTCT ACTGCCCTCCCGAGGCGTCCGTGCTCTTGGCGTCATATGCTGTCCAGGCCAAGTATGGCGACTATGACCC CTCTGTGCACAAGCGGGGATTTTTAGCCCAAGAGGAATTGCTCCCGAAAAGGGTGATAAATCTCTATCAG ATGACTCCGGAAATGTGGGAGGAGAGAATTACGGCTTGGTATGCGGAGCACCGGGGCAGAGCCAGGGATG AAGCTGAAATGGAGTATTTGAAGATAGCTCAGGACCTGGAGATGTATGGTGTGAACTACTTTACAATCCG GAATAAAAAGGGCACAGAGTTGCTGCTTGGAGTGGATGCTCTTGGGCTTCATATCTATGACCCTGAGAAC AGGCTGACCCCCAAGATCTCCTTCCCATGGAATGAAATCCGAAACATCTCCTACAGCGACAAGGAGTTTA CTATTAAACCACTGGATAAGAAAATTGATGTCTTCAAATTTAACTCCTCAAAGCTTCGTGTTAATAAGCT GATTCTTCAGCTATGTATTGGGAACCATGACCTATTTATGAGGCGACGGAAAGCTGACTCTTTAGAAGTT CAGCAGATGAAAGCCCAGGCCAGGGAAGAGAAGGCTAGAAAGCAGATGGAAAGGCAGCGGCTGGCTCGAG AGAAGCAGATGCGGGAGGAGGCCGAGCGTACAAGAGATGAGTTAGAGAGGAGGCTCCTGCAGATGAAAGA AGAAGCAACGATGGCCAATGAAGCTCTGATGCGCTCTGAGGAGACAGCTGATCTGTTGGCTGAAAAGGCT CAGATCACAGAGGAGGAGGCCAAGCTTTTGGCACAAAAGGCTGCAGAGGCTGAGCAAGAGATGCAGCGAA TCAAGGCCACGGCCATTCGGACAGAGGAGGAGAAGCGCCTGATGGAGCAGAAGGTGCTGGAGGCTGAAGT GCTGGCATTGAAGATGGCTGAGGAGTCAGAGAGGAGGGCCAAGGAGGCTGATCAGTTAAAGCAAGACTTG CAAGAAGCCAGAGAAGCAGAGCGAAGAGCCAAGCAGAAGCTCTTAGAAATCGCCACCAAGCCCACCTATC CACCCATGAACCCAATTCCACCACCACTGCCTCCTGACATACCGAGCTTCGACATTATTGCTGACAGCTT GTCATTTGACTTCAAGGATACGGACATGAAGCGACTTTCCATGGAGATAGAGAAAGAAAAAGTGGAGTAC ATGGAGAAGAGCAAGCACCTGCAGGAGCAGCTCAACGAGCTCAAGACGGAGATCGAGGCCTTGAAACTCA AAGAGCGGGAGACGGCCTTGGACGTCCTACACAGCGAGAGCTCAGACAGAGGCGGCCCCAGCAGCAAGCA TAATACCATTAAAAAGGTACCTGAAATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252253
Insert Size	1641 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252253.1, NP_001239182.1
RefSeq Size	1846 bp
RefSeq ORF	1641 bp
Locus ID	18016
UniProt ID	P46662
Gene Summary	Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex (By similarity). Plays a role in lens development and is required for complete fiber cell terminal differentiation, maintenance of cell polarity and separation of the lens vesicle from the corneal epithelium.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) differs in the 5' UTR, 3' UTR, and coding region, compared to variant 1. The resulting isoform (4) is shorter at the N-terminus and has a shorter and distinct C-terminus, compared to isoform 1.

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