Yes1 (NM_001205133) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	p61-Yes; Yes
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228232 representing NM_001205133 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTGCATTAAAAGTAAAGAAAACAAAAGTCCAGCCATAAAATACACACCGGAAAATCTTACAGAGC CTGTAAGCCCAAGTGCCAGTCATTATGGAGTGGAACATGCTACAGTTGCCCCGACCTCTTCCACAAAGGG AGCATCAGTTAATTTTAACAGTCTTTCCATGACACCCTTTGGAGGGTCCTCAGGGGTGACTCCTTTTGGA GGAGCGTCTTCCTCATTCTCAGTGGTGTCAAGTTCATATCCTACAGGTTTAACAGGTGGTGTCACTATAT TTGTGGCCTTGTATGATTATGAAGCTAGAACTACAGAAGACCTTTCCTTTAAGAAGGGTGAACGATTTCA AATAATTAACAATACGGAAGGAGACTGGTGGGAAGCAAGATCAATTGCTACCGGAAAGAGTGGTTATATC CCTAGCAATTACGTAGTGCCTGCAGATTCCATTCAGGCAGAAGAATGGTATTTTGGCAAAATGGGGAGAA AAGATGCGGAAAGATTACTTCTGAATCCTGGGAATCAGCGAGGTATTTTCTTAGTAAGAGAAAGTGAAAC TACTAAAGGTGCTTACTCCCTCTCAATCCGTGATTGGGATGAGGTGAGGGGTGACAATGTGAAGCATTAC AAGATCAGAAAACTTGACAATGGTGGCTACTACATCACGACCAGAGCTCAGTTTGATACACTGCAGAAGC TGGTGAAGCACTACACAGAACATGCTGATGGATTATGCCACAAGTTAACAACTGTGTGTCCTACTGTGAA ACCCCAGACTCAAGGTCTGGCAAAAGATGCTTGGGAAATCCCTCGAGAATCATTGCGACTAGAGGTGAAA CTAGGTCAAGGATGCTTTGGGGAAGTGTGGATGGGAACATGGAATGGAACTACAAAAGTAGCAATCAAAA CACTAAAGCCAGGTACAATGATGCCAGAAGCATTCCTTCAAGAAGCTCAGATAATGAAAAAGCTAAGACA CGATAAACTTGTTCCACTCTATGCAGTTGTTTCTGAAGAGCCCATTTATATTGTCACCGAGTTTATGTCA AAAGGAAGCTTGTTAGATTTCCTTAAAGAAGGAGATGGAAAGTATTTGAAGCTTCCACAGCTGGTTGATA TGGCTGCTCAGATCGCTGATGGCATGGCGTATATTGAAAGAATGAACTATATTCACCGAGATCTCCGAGC TGCTAATATTCTTGTAGGAGAAAATCTTATATGCAAAATAGCAGATTTTGGCTTAGCAAGATTAATTGAA GACAATGAATACACGGCAAGACAAGGTGCAAAATTTCCAATCAAGTGGACAGCTCCTGAGGCTGCTCTGT ATGGTCGATTTACAATAAAGTCAGATGTGTGGTCATTTGGAATTCTACAGACAGAGCTGGTAACAAAAGG AAGAGTGCCATATCCAGGTATGGTAAACCGTGAAGTATTGGAACAAGTAGAGCGGGGATACAGAATGCCT TGCCCCCAGGGCTGTCCCGAATCCCTCCATGAATTGATGAATCTTTGCTGGAAGAAGGATCCTGATGAAA GACCAACATTTGAATATATTCAGTCCTTCTTGGAAGACTACTTCACTGCTACAGAGCCACAGTACCAACC AGGAGAAAATTTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001205133
Insert Size	1626 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001205133.1, NP_001192062.1
RefSeq Size	2432 bp
RefSeq ORF	1626 bp
Locus ID	22612
UniProt ID	Q04736
Gene Summary	Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in both the 5' UTR and 3' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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