Gabrq (NM_001290435) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228174 representing NM_001290435 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCATCCGAGGTATGCTGCGAGCCGCTGCGCTCCTGCTGCTCATTAGGACTTGGCTGGCAGAGAGTA ACGGCCCCAGTCCCACCCCGAAATTCCACTTCGAGCTCTCCTCCAGCACGCCTGAAGTCATTCTGGATCT CTTCAACTGCAAAAACTGTGCAAATGAAGCTGTGGTTCAAAAGATTTTGGACAGGGTGCTGTCAACATAT GATGTCCGACTTAGACCAAATTTTGGAGGAGCTCCTGTGCCTGTGTCAGTATCTATCTATGTGTCCAGTA TTGAACAGATCTCAGAAATAAATATGGACTATACAATCACAATGTTTTTGCATCAGACCTGGAAGGACAC ACGCTTAGCTTACTATGAAACCAACCTAAACCTGACCCTGGATTATCGGATGCATGAGAAGCTCTGGGTT CCTGACTGCTACTTTGTGAATAGCAAAGATGCCTTTGTGCATGATGTAACTGTGGAGAATCGTGTATTTC AACTTCACCCAGATGGGACAGTGCGATATGGCATCCGACTTACCACAACAGCAGCCTGTTCCCTGGATCT GCAAAAATTCCCTATGGACAAACAATCCTGCAAGCTGGAGGTGGAGAGCTATGGCTACACTGTCGAAGAC ATTGTGTTATCCTGGGAAGATGATAATGCCATCCACATTACTGATGGGCTGCATATCCCTCAGTACACGT ACCTGGGGAGAACAATTACCAGCAAGGAAGTGTACTTCTACACAGGCTCCTACATGCGCCTGATAGTGAA GTTCCAGGTTCAGAGGGAAGTCCGCAGTTACCTTGTGCAGGTCTATTGGCCCACGGTCCTCACCACCATT CTCTCCTGGATATCATTTTGGATGAACTATGATTCCTCTGCAGCCAGGGTAACTATTGGACTAACTTCGA TCCTTGTCCTGACTACCATCGACTCACATATGCGGGATAAACTCCCCCATATTTCCTGTATCAAAGCTAT TGACATCTACATCCTTGTATGCCTATTCTTCGTTTTCCTGTCACTGCTGGAGTATGTCTACATCAACTAC CTTTTCTTCAGCCAAGTACCTCGGCGTAATCACAGAAGATGCAGGAAACCCAGGAGAGTTGTTGCCCGAT ACCGCTACCAAGAAGTGGTGGTAGCAAATGTGCAGGATGGCCTGATTAATGTGGAAGACAGAGTAGAAGA CAGAGCTGGTCCCCTTCCTGACAGCCCCATGCAGGCTCATCTGGCAAGCCAGGAAAGTCTAGGATCCTTG GTGTTCACATCAGAGCAGGCCCAGCTGGCTACCTCAGAAAGTCTCAGTCTACTCTCTTCTGCCTCAAGCC AGACTCAATTGGCCACTGGAGAAAGCCTGAGTGATCTGCCCTCCACCTCTGAGCAAACAGTGCCTGAGTG TACCATTCACTTTCATGGCTTTCTTACTAATGACAGTATTATCCCCATCAAAATCCACAGCCGATCTGAT GCCTGTGATGATGAAGACTCCGAAGAAAGCCTAAGCTCTGAGGAGAGCCATGGCCATGGAAGCAGCCACA CTGGAAGGCTCAAGCTCCAGATTAGCCAGAGTGCATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290435
Insert Size	1578 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290435.1, NP_001277364.1
RefSeq Size	4943 bp
RefSeq ORF	1578 bp
Locus ID	57249
Gene Summary	GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the shorter isoform (a).

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