Scarb1 (NM_001205083) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI120173; CD36; Cd36l1; Chohd1; Cla-1; Cla1; D5Ertd460e; Hdlq1; Hlb398; mSR-BI; SR-B1; SR-BI
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228139 representing NM_001205083 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGGCAGCTCCAGGGCGCGCTGGGTGGCCTTGGGGTTGGGCGCCCTGGGGCTGCTGTTTGCTGCGC TCGGCGTTGTCATGATCCTCATGGTGCCCTCCCTCATCAAGCAGCAGGTGCTCAAGAATGTCCGCATAGA CCCGAGCAGCCTGTCCTTCGGGATGTGGAAGGAGATCCCCGTCCCTTTCTACTTGTCTGTCTACTTCTTC GAAGTGGTCAACCCAAACGAGGTCCTCAACGGCCAGAAGCCAGTAGTCCGGGAGCGTGGACCCTATGTCT ACAGGGAGTTCAGACAAAAGGTCAACATCACCTTCAATGACAACGACACCGTGTCCTTCGTGGAGAACCG CAGCCTCCATTTCCAGCCTGACAAGTCGCATGGCTCAGAGAGTGACTACATTGTACTGCCTAACATCTTG GTCCTGGGGGGCTCGATATTGATGGAGAGCAAGCCTGTGAGCCTGAAGCTGATGATGACCTTGGCGCTGG TCACCATGGGCCAGCGTGCTTTTATGAACCGCACAGTTGGTGAGATCCTGTGGGGCTATGACGATCCCTT CGTGCATTTTCTCAACACGTACCTCCCAGACATGCTTCCCATAAAGGGCAAATTTGGCCTGTTTGTTGGG ATGAACAACTCGAATTCTGGGGTCTTCACTGTCTTCACGGGCGTCCAGAATTTCAGCAGGATCCATCTGG TGGACAAATGGAACGGACTCAGCAAGATCGATTATTGGCATTCAGAGCAGTGTAACATGATCAATGGGAC TTCCGGGCAGATGTGGGCACCCTTCATGACACCCGAATCCTCGCTGGAATTCTTCAGCCCGGAGGCATGC AGGTCCATGAAGCTGACCTACAACGAATCAAGGGTGTTTGAAGGCATTCCCACGTATCGCTTCACGGCCC CCGATACTCTGTTTGCCAACGGGTCCGTCTACCCACCCAACGAAGGCTTCTGCCCATGCCGAGAGTCTGG CATTCAGAATGTCAGCACCTGCAGGTTTGGTGCGCCTCTGTTTCTCTCCCACCCCCACTTTTACAACGCC GACCCTGTGTTGTCAGAAGCTGTTCTTGGTCTGAACCCTAACCCAAAGGAGCATTCCTTGTTCCTAGACA TCCATCCGGTCACTGGGATCCCCATGAACTGTTCTGTGAAGATGCAGCTGAGCCTCTACATCAAATCTGT CAAGGGCATCGGGCAAACAGGGAAGATCGAGCCAGTAGTTCTGCCGTTGCTGTGGTTCGAACAGAGCGGA GCAATGGGTGGCAAGCCCCTGAGCACGTTCTACACGCAGCTGGTGCTGATGCCCCAGGTTCTTCACTACG CGCAGTATGTGCTGCTGGGGCTTGGAGGCCTCCTGTTGCTGGTGCCCATCATCTGCCAACTGCGCAGCCA GGTAAGTAGGAGGGGCGGCCACGCCTCGGACTCGGCTCGGGTTTCAGCCGACCTCTGTTTCCTGCAGCTA GCTCTTGTTCTACCTCCTCTCTCGCACCCTGCAGTAACTTTTCTCTACAAGTCCTGGAAGGCCCAGGACC CTCCCAGGTCGGGGCTAGGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001205083
Insert Size	1563 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001205083.1, NP_001192012.1
RefSeq Size	2196 bp
RefSeq ORF	1563 bp
Locus ID	20778
Gene Summary	Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells (By similarity). Both isoform 1 and isoform 2 act as receptors for HDL, mediating selective uptake of cholesteryl ether and HDL-dependent cholesterol efflux (PubMed:9254074, PubMed:9614139). Also facilitates the flux of free and esterified cholesterol between the cell surface and apoB-containing lipoproteins and modified lipoproteins, although less efficiently than HDL. May be involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs at the 3' end compared to variant 1. This results in a frame-shift and a longer isoform (3) with a distinct C-terminus compared to isoform 1.

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