Slc7a7 (NM_001253680) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI790233; my+lat1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228104 representing NM_001253680 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCAACAGCACCAAGTATGAAGTGGCTGCTCAGCACGAGGCCGACGATGGCTCTGCTCTCGGGGATG GGGCCAGCCCAGTGGCGGAGCAGGTCAAACTGAAGAAGGAGATCTCCCTGCTTAATGGCGTGTGTCTCAT AGTGGGGAACATGATCGGCTCCGGCATCTTTGTCTCCCCTAAGGGTGTGCTCATGTACAGTGCCTCTTTT GGCCTCTCACTGGTCATCTGGGCTGTCGGGGGCATTTTCTCCGTCTTTGGGGCCCTTTGTTACGCTGAAC TGGGTACCACCATTAAGAAATCCGGGGCCAGCTATGCTTATATCCTGGAGGCCTTCGGGGGATTCCTTGC GTTCATCCGCCTCTGGACTTCTCTGCTCATCATTGAACCCACCAGCCAGGCCGTCATTGCCATCACCTTT GCCAACTACATGGTGCAGCCCCTCTTCCCGAGCTGTGGCGCTCCCTATGCCGCCGGCCGCCTGCTGGCCG CTGCCTGCATCTGTCTCTTAACCTTCATTAACTGTGCCTATGTCAAGTGGGGAACCCTGGTCCAAGATAT TTTCACCTATGCTAAGGTGTTGGCGCTGATTGCAGTCATCATTGCAGGCATTGTTAGACTTGGCCAGGGA GCCACAGCTAACTTTGAGAACTCCTTTGAGGGCTCATCCTTTGCAATGGGTGACATTGCTCTGGCACTCT ACTCAGCCCTGTTCTCCTACTCGGGCTGGGACACCCTTAACTATGTCACCGAAGAGATCAGGAACCCCGA GAGGAACTTGCCCCTCTCCATTGGCATTTCCATGCCAATCGTCACCATCATCTACCTCTTGACCAATGTG GCCTATTACAGTGTGCTAGACATAAAGGAAATCCTGGCCAGTGACGCTGTTGCCGTGACCTTTGCAGATC AAATTTTTGGAGTATTCAATTGGATAATTCCAGTAGCGGTTGCATTCTCTTGCTTTGGTGGGCTCAACGC CTCCATCGTGGCTGCTTCCAGGCTTTTATTTGTGGGCTCGAGAGAAGGCCACCTCCCTGACGCCATCTGT ATGGTTCATGTTGAACGGTTCACACCAGTGCCTTCTCTACTCTTCAATGGTGTGTTGTCCCTGGTCTACC TGTGCGTGGAGGACATCTTCCAGCTCATTAACTACTATAGCTTCAGCTACTGGTTCTTTGTGGGCCTTTC TATTGTGGGTCAGCTTTATCTACGCTGGAAGGACCCCGACCGGCCTCGCCCTCTCAAGCTCAGCCTTTTC TTCCCCATCATCTTCTGCCTCTGCACCATCTTCCTGGTGGCTGTTCCACTTTACAGCGACACCATCAACT CCCTCATCGGCATTGGCATTGCACTCTCAGGCCTGCCCTTCTACTTCTTCATCATCAGAGTGCCAGAGCA CAAACGACCACTGTTCCTCCGCAGGATTGTAGCCTCTATCACACGGTACCTCCAGATCCTCTGTATGTCA GTTGCTGCAGAAATGGACTTGGAAGATGGAGAGTTGTCCAAACAAGATCCCAAGTCTAAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001253680
Insert Size	1533 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001253680.1, NP_001240609.1
RefSeq Size	2152 bp
RefSeq ORF	1533 bp
Locus ID	20540
UniProt ID	Q9Z1K8
Gene Summary	Involved in the sodium-independent uptake of dibasic amino acids and sodium-dependent uptake of some neutral amino acids. Requires coexpression with SLC3A2/4F2hc to mediate the uptake of arginine, leucine and glutamine. Plays a role in nitric oxide synthesis via transport of L-arginine, and is involved in the transport of L-arginine in monocytes.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1-3 encode the same protein.

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