Banp (NM_001285981) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AA408158; SMAR1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228097 representing NM_001285981 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGTCAGAGCAGGACCTGGCGGATGTGGTTCAGATTGCAGTGGAAGACCTGAGCCCTGATCACCCAG TTGTTTTGGAGAATCATGTCGTGACAGATGATGATGAACCTGCCTTGAAGCGCCAGCGACTAGAGATCAA TTGCCAGGACCCCTCTATAAAGTCTTTCCTGTACTCTATTAACCAGACGATATGTTTGCGGTTGGATAGC ATTGAGGCCAAGCTGCAAGCTCTCGAGGCCACTTGCAAATCTCTGGAAGAGAAGCTAGACCTGGTCACCA ATAAACAGCACAGTCCCATCCAGGTCCCCATGGTGGCAGGTTCCCCACTTGGCGCCACCCAGACCTGCAA CAAAGTGCGATGCGCTGTTCCTGGGCGTCGGCAGAACACCATCGTGGTGAAAGTGCCTGGTCAGGACGAC AGCCACAACGAAGATGGGGAGAGCGGGTCAGAGGCCAGTGACTCCGTGTCTAACTGTGGCCAGCCAGGAA GCCAGAACATTGGAAGCAACGTCACACTCATCACCCTGAACTCCGAAGAGGACTATCCCAATGGCACCTG GCTGGGCGATGAGAATAACCCTGAGATGCGGGTACGCTGTGCCATCATCCCTTCCGACATGTTGCACATC AGCACCAACTGTCGCACGGCCGAGAAGATGGCGCTGACACTGCTGGACTACCTGTTCCACCGTGAGGTGC AGGCTGTGTCCAACTTGTCCGGCCAGGGCAAGCACGGGAAGAAGCAGCTGGACCCCCTCACCATCTACGG CATCCGGTGTCACCTCTTCTATAAATTTGGAATCACGGAATCTGACTGGTATCGGATCAAGCAGAGCATT GACTCCAAGTGCCGGACAGCCTGGCGGCGGAAGCAGCGAGGCCAGAGCCTGGCGGTCAAGAGCTTCTCTC GGAGGACGCCATCCTCATCCTCTTACAGTGCCTCAGAGACCATGATGGGAACCCCTCCTCCCACCAGTGA GCTACAGCAGTCACAGCCACAGGCCCTACACTACGCCCTGGCCAACGCCCAGCAGGTCCAGATCCACCAG ATTGGGGAGGATGGACAGGTGCAAGTAATCCCACAGGGCCACCTCCACATTGCCCAGGTGCCTCAAGGGG AGCAGGTGCAGATCACACAGGACAGCGAGGGCAATCTGCAGATCCATCATGTGGGTCAGGATGGCCAGTC GTGGGGCCTGTGCCAGAATCCCATTCCTGTCAGCGGTGACTCAGTGGCCCAGGCTAATCCCTCCCAGCTT TGGCCTCTGGGAGGAGACACACTTGATCTGCCTGCTGGAAATGAAATGATCCAGGTACTGCAGGGTGCTC AGCTCATAGCCGTGGCCTCTTCAGACCCTGCTGCTACAGGAGTAGATGGGTCGCCTCTCCAGGGCAGTGA CATTCAGGTTCAGTATGTCCAGCTGGCGCCTGTGAGTGACCACACAGCCGCAGCGCAGACCGCAGAGGCC CTGCAGCCCACTCTGCAGCCCGACATGCAGCTTGAACATGGGGCCATCCAGATCCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001285981
Insert Size	1530 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001285981.1, NP_001272910.1
RefSeq Size	5381 bp
RefSeq ORF	1530 bp
Locus ID	53325
UniProt ID	Q8VBU8
Gene Summary	Controls V(D)J recombination during T-cell development by repressing T-cell receptor (TCR) beta enhancer function. Binds to scaffold/matrix attachment region beta (S/MARbeta), an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Represses cyclin D1 transcription by recruiting HDAC1 to its promoter, thereby diminishing H3K9ac, H3S10ph and H4K8ac levels. Promotes TP53 'Ser-15' phosphorylation and nuclear accumulation, which causes cell cycle arrest and inhibits tumor growth.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks an alternate in-frame exon in the coding region compared to variant 2. The resulting protein (isoform 3) is shorter but has the same N- and C-termini compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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