Rbck1 (NM_019705) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AL033326; HOIL-1; HOIL-1L; Ubce7ip3; UIP28
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228090 representing NM_019705 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGAGAAGACCAAGAAAGCAGAGGAGATGGCCCTGAGCCTTGCCCGGGCAGTGGCTGGCGGAGATG AACAGGCTGCTATCAAGTATGCCACCTGGCTGGCAGAGCAGAGGGTGCCCCTCAGGGTGCAAGTAAAACC CGAGGTCTCCCCAACACAGGACATCAGGCTCTGTGTGAGTGTGGAGGATGCGTACATGCACACCGTCACC ATTTGGCTCACAGTACGCCCGGATATGACAGTGGCCTCCCTTAAGGACATGGTATTCCTGGACTATGGCT TCCCACCTAGCCTGCAGCAGTGGGTGGTTGGACAGAGGCTGGCACGAGACCAGGAGACCTTGCATTCACA CGGCATTCGGCGGAATGGAGACGGTGCCTATCTCTACCTGCTGTCAGCCCGCAACACGTCACTCAACCCA CAAGAGCTGCAGCGGCAGCGGCAACTGCGAATGTTGGAAGATTTGGGCTTCAAGGACCTCACCCTTCAGT CACGGGGGCCCTTGGAACCTGTCCTTCCGAAGCCCAGGACCAACCAGGAGCCGGGACAGCCAGATGCAGC ACCGGAGTCACCACCGGTGGGCTGGCAGTGCCCTGGCTGCACTTTCATCAACAAACCCACACGGCCTGGG TGTGAGATGTGCTGTCGTGCAAGGCCTGAGACCTACCAGATACCTGCTTCATACCAGCCTGACGAGGAAG AGCGAGCACGCCTGGCCGGCGAGGAGGAGGCGCTGCGCCAGTACCAGCAGCGGAAACAGCAGCAGCAGGA GGGGAACTACCTGCAGCACGTGCAGCTGGAGCAGAGGAGCCTGGTGCTGAACACCGAACCTACTGAGTGC CCTGTGTGCTACTCAGTGCTGGCACCCGGCGAGGCCGTGGTGCTGCGTGAGTGTCTGCACACCTTCTGCA GGGAGTGCCTGCAGGGCACCATCCGAAACAGCCAGGAGGCGGAGGTGGCTTGCCCCTTCATTGACAGCAC CTACTCATGCCCCGGCAAGCTGCTGGAGAGAGAGATCCGGGCGCTCCTGTCCCCTGAGGACTACCAGCGT TTCCTGGATCTGGGTGTGTCCATCGCAGAGAACCGAAGCACCTTGAGCTACCACTGCAAGACCCCCGACT GCAGGGGTTGGTGCTTCTTTGAGGATGATGTCAACGAGTTCACCTGTCCTGTGTGCACCCGTGTCAACTG CCTGCTCTGCAAGGCCATCCATGAGCACATGAATTGCAGGGAGTACCAAGACGACCTGGCCCTGCGGGCT CAGAATGATGTGGCTGCCCGGCAGACGACAGAGATGCTAAAGGTAATGCTGCAGCAGGGCGAGGCCATGC ACTGCCCACAGTGCCGGATTGTGGTGCAGAAGAAGGACGGCTGTGACTGGATCCGCTGTACAGTCTGCCA CACTGAGATCTGCTGGGTCACCAAGGGCCCACGCTGGGGCCCAGGGGGCCCAGGGGACACCAGTGGTGGC TGCCGCTGCCGGGTCAATGGGATTCCATGCCACCCAAGCTGTCAAAACTGCCACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_019705
Insert Size	1527 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_019705.3, NP_062679.2
RefSeq Size	2251 bp
RefSeq ORF	1527 bp
Locus ID	24105
UniProt ID	Q9WUB0
Gene Summary	E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. Functions as an E3 ligase for oxidized IREB2 and both heme and oxygen are necessary for IREB2 ubiquitination. Promotes ubiquitination of TAB2 and IRF3 and their degradation by the proteasome. Component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with OTULIN, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. Binds polyubiquitin of different linkage types (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same isoform (a).

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