Rbck1 (NM_019705) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AL033326; HOIL-1; HOIL-1L; Ubce7ip3; UIP28
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230301 representing NM_019705 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGAGAAGACCAAGAAAGCAGAGGAGATGGCCCTGAGCCTTGCCCGGGCAGTGGCTGGCGGAGATG AACAGGCTGCTATCAAGTATGCCACCTGGCTGGCAGAGCAGAGGGTGCCCCTCAGGGTGCAAGTAAAACC CGAGGTCTCCCCAACACAGGACATCAGGCTCTGTGTGAGTGTGGAGGATGCGTACATGCACACCGTCACC ATTTGGCTCACAGTACGCCCGGATATGACAGTGGCCTCCCTTAAGGACATGGTATTCCTGGACTATGGCT TCCCACCTAGCCTGCAGCAGTGGGTGGTTGGACAGAGGCTGGCACGAGACCAGGAGACCTTGCATTCACA CGGCATTCGGCGGAATGGAGACGGTGCCTATCTCTACCTGCTGTCAGCCCGCAACACGTCACTCAACCCA CAAGAGCTGCAGCGGCAGCGGCAACTGCGAATGTTGGAAGATTTGGGCTTCAAGGACCTCACCCTTCAGT CACGGGGGCCCTTGGAACCTGTCCTTCCGAAGCCCAGGACCAACCAGGAGCCGGGACAGCCAGATGCAGC ACCGGAGTCACCACCGGTGGGCTGGCAGTGCCCTGGCTGCACTTTCATCAACAAACCCACACGGCCTGGG TGTGAGATGTGCTGTCGTGCAAGGCCTGAGACCTACCAGATACCTGCTTCATACCAGCCTGACGAGGAAG AGCGAGCACGCCTGGCCGGCGAGGAGGAGGCGCTGCGCCAGTACCAGCAGCGGAAACAGCAGCAGCAGGA GGGGAACTACCTGCAGCACGTGCAGCTGGAGCAGAGGAGCCTGGTGCTGAACACCGAACCTACTGAGTGC CCTGTGTGCTACTCAGTGCTGGCACCCGGCGAGGCCGTGGTGCTGCGTGAGTGTCTGCACACCTTCTGCA GGGAGTGCCTGCAGGGCACCATCCGAAACAGCCAGGAGGCGGAGGTGGCTTGCCCCTTCATTGACAGCAC CTACTCATGCCCCGGCAAGCTGCTGGAGAGAGAGATCCGGGCGCTCCTGTCCCCTGAGGACTACCAGCGT TTCCTGGATCTGGGTGTGTCCATCGCAGAGAACCGAAGCACCTTGAGCTACCACTGCAAGACCCCCGACT GCAGGGGTTGGTGCTTCTTTGAGGATGATGTCAACGAGTTCACCTGTCCTGTGTGCACCCGTGTCAACTG CCTGCTCTGCAAGGCCATCCATGAGCACATGAATTGCAGGGAGTACCAAGACGACCTGGCCCTGCGGGCT CAGAATGATGTGGCTGCCCGGCAGACGACAGAGATGCTAAAGGTAATGCTGCAGCAGGGCGAGGCCATGC ACTGCCCACAGTGCCGGATTGTGGTGCAGAAGAAGGACGGCTGTGACTGGATCCGCTGTACAGTCTGCCA CACTGAGATCTGCTGGGTCACCAAGGGCCCACGCTGGGGCCCAGGGGGCCCAGGGGACACCAGTGGTGGC TGCCGCTGCCGGGTCAATGGGATTCCATGCCACCCAAGCTGTCAAAACTGCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230301 representing NM_019705 Red=Cloning site Green=Tags(s) MDEKTKKAEEMALSLARAVAGGDEQAAIKYATWLAEQRVPLRVQVKPEVSPTQDIRLCVSVEDAYMHTVT IWLTVRPDMTVASLKDMVFLDYGFPPSLQQWVVGQRLARDQETLHSHGIRRNGDGAYLYLLSARNTSLNP QELQRQRQLRMLEDLGFKDLTLQSRGPLEPVLPKPRTNQEPGQPDAAPESPPVGWQCPGCTFINKPTRPG CEMCCRARPETYQIPASYQPDEEERARLAGEEEALRQYQQRKQQQQEGNYLQHVQLEQRSLVLNTEPTEC PVCYSVLAPGEAVVLRECLHTFCRECLQGTIRNSQEAEVACPFIDSTYSCPGKLLEREIRALLSPEDYQR FLDLGVSIAENRSTLSYHCKTPDCRGWCFFEDDVNEFTCPVCTRVNCLLCKAIHEHMNCREYQDDLALRA QNDVAARQTTEMLKVMLQQGEAMHCPQCRIVVQKKDGCDWIRCTVCHTEICWVTKGPRWGPGGPGDTSGG CRCRVNGIPCHPSCQNCH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_019705
ORF Size	1524 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_019705.3, NP_062679.2
RefSeq Size	2251 bp
RefSeq ORF	1527 bp
Locus ID	24105
UniProt ID	Q9WUB0
MW	57.5 kDa
Gene Summary	E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. Functions as an E3 ligase for oxidized IREB2 and both heme and oxygen are necessary for IREB2 ubiquitination. Promotes ubiquitination of TAB2 and IRF3 and their degradation by the proteasome. Component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with OTULIN, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. Binds polyubiquitin of different linkage types (By similarity).[UniProtKB/Swiss-Prot Function]

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