Ephx2 (NM_001271402) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	CEH; Eph2; SEH; sEP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228054 representing NM_001271402 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGGGAAGATCACATTTTCGCAGTGGGTACCACTCATGGATGAAAGCTACAGGAAGTCCTCCAAAG CCTGTGGAGCCAATCTACCTGAGAATTTCTCCATAAGTCAAATATTCAGCCAAGCTATGGCAGCAAGAAG CATCAACCGCCCCATGCTTCAGGCAGCCATTGCTCTCAAAAAGAAAGGATTCACAACATGCATTGTCACC AACAACTGGCTGGACGACGGAGACAAGAGAGACAGCCTGGCCCAGATGATGTGTGAGCTGAGCCAACACT TTGACTTCCTGATAGAGTCCTGTCAGGTTGGGATGATCAAGCCTGAGCCTCAGATCTACAATTTTTTACT GGATACCCTGAAGGCAAAACCCAATGAGGTTGTTTTCCTAGATGACTTTGGAAGTAATCTGAAGCCAGCC CGTGACATGGGGATGGTTACCATCCTGGTCCACAACACAGCCTCCGCTCTGAGAGAACTGGAGAAGGTCA CAGGGACACAGTTTCCTGAGGCCCCACTGCCAGTCCCATGCAATCCAAATGACGTCAGCCATGGATATGT GACAGTGAAGCCAGGGATCCGCCTGCATTTTGTGGAGATGGGCTCTGGCCCTGCCCTATGCCTTTGCCAT GGGTTTCCTGAGAGCTGGTTTTCTTGGAGGTACCAGATCCCTGCTCTGGCCCAGGCAGGCTTTCGTGTTC TGGCTATAGACATGAAAGGCTATGGAGACTCATCTTCTCCTCCTGAAATAGAAGAATATGCCATGGAATT GCTGTGTAAGGAGATGGTGACATTCCTGGATAAGCTGGGAATCCCTCAAGCAGTGTTCATTGGCCATGAC TGGGCTGGTGTGATGGTGTGGAACATGGCTCTCTTCTACCCTGAGAGAGTGAGGGCTGTGGCCAGTTTGA ACACGCCGTTTATGCCACCAGATCCTGATGTGTCTCCCATGAAAGTTATCCGATCTATCCCAGTTTTCAA TTATCAGCTGTACTTTCAAGAACCAGGAGTGGCCGAGGCTGAACTGGAGAAGAACATGAGTCGGACTTTC AAAAGCTTCTTCCGAGCCAGTGATGAGACAGGTTTCATCGCTGTGCATAAAGCCACTGAAATAGGGGGAA TCCTTGTGAATACTCCAGAAGATCCCAACCTCAGCAAAATTACTACTGAGGAAGAAATAGAGTTTTACAT ACAGCAGTTCAAGAAGACTGGCTTCAGAGGTCCTCTGAACTGGTACCGGAACACAGAAAGAAACTGGAAG TGGAGCTGTAAAGGGTTGGGACGAAAGATCTTGGTCCCAGCCCTGATGGTCACAGCTGAGAAGGACATTG TACTCCGTCCTGAAATGTCCAAGAACATGGAAAAGTGGATCCCTTTCCTGAAAAGGGGACACATTGAAGA CTGTGGTCACTGGACACAGATAGAGAAACCAACTGAGGTGAACCAGATTCTCATCAAGTGGCTGCAGACT GAAGTCCAGAACCCATCAGTGACCTCCAAGATTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271402
Insert Size	1506 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271402.1, NP_001258331.1
RefSeq Size	2142 bp
RefSeq ORF	1506 bp
Locus ID	13850
Gene Summary	Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo-9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro-9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy-octadec-9Z-enoic acid and 12-phosphonooxy-octadec-9E-enoic acid.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate splice site at the 5' exon, differs in the 5' UTR, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) has a shorter N-terminus, compared to isoform a.

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